Centrifugation Assisted Agrobacterium tumefaciens-mediated Transformation (CAAT) of embryogenic cell suspensions of banana (Musa spp. Cavendish AAA and Lady finger AAB)

Khanna, Harjeet; Becker, Douglas K.; Kleidon, Jennifer; Dale, James L.

doi:10.1023/b:molb.0000047771.34186.e8

Cited by 115 publications

(97 citation statements)

References 22 publications

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“…The transformable tissue of choice for bananas is embryogenic cell suspensions derived from immature male flowers [49]. While the development of these cell suspensions is a lengthy process (Figure 2), it is probable that, with sufficient effort, it should be possible to generate them from most cultivars.…”

Section: Conventional Breeding or Genetic Modificationmentioning

confidence: 99%

“…Other cultivars from laboratories include the East African Highland banana, Gonja (plantain), and Rasthali. Additionally, efficient transformation protocols are available for bananas, for both Agrobacterium-mediated transformation and microprojectile bombardment [49][50][51]. There are a number of laboratories around the world that have established banana transformation pipelines, including ours at Queensland University of Technology (https://www.qut.edu.au/), the IITA in Kenya (http://newint.iita.org/), NARO in Uganda (http://www.naro.go.ug/), the Bhabha Atomic Research Centre in India (http://www.barc.gov.in/), and the Catholic University of Leuven in Belgium (https://www.kuleuven.be/english).…”

Section: Conventional Breeding or Genetic Modificationmentioning

confidence: 99%

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Modifying Bananas: From Transgenics to Organics?

et al. 2017

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Bananas are one of the top ten world food crops. Unlike most other major food crops, bananas are difficult to genetically improve. The challenge is that nearly all banana cultivars and landraces are triploids, with high levels of male and female infertility. There are a number of international conventional breeding programs and many of these are developing new cultivars. However, it is virtually impossible to backcross bananas, thus excluding the possibility of introgressing new traits into a current cultivar. The alternative strategy is to "modify" the cultivar itself. We have been developing the capacity to modify Cavendish bananas and other cultivars for both disease resistance and enhanced fruit quality. Initially, we were using transgenes; genes that were derived from species outside of the Musa or banana genus. However, we have recently incorporated two banana genes (cisgenes) into Cavendish; one to enhance the level of pro-vitamin A and the other to increase the resistance to Panama disease. Modified Cavendish with these cisgenes have been employed in a field trial. Almost certainly, the next advance will be to edit the Cavendish genome, to generate the desired traits. As these banana cultivars are essentially sterile, transgene flow and the outcrossing of modified genes into wild Musa species. are highly unlikely and virtually impossible in other triploid cultivars. Therefore, genetic changes in bananas may be compatible with organic farming.

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Section: Conventional Breeding or Genetic Modificationmentioning

confidence: 99%

Section: Conventional Breeding or Genetic Modificationmentioning

confidence: 99%

Modifying Bananas: From Transgenics to Organics?

et al. 2017

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“…Lane 1-7 were colonies of A. tumefaciens transformed with pARTC, whereas lane P was pARTC used as a positive control. The nptII primers used in this study based on nptII primers designed by Khanna et al (2004) to obtain PCR product of 659 bp. Electrophoregram showed that transformed A. tumefaciens strain GV3101 contained pARTC.…”

Section: Molecular Analysis Of Phac Gene In Partc Plasmid Nptii Andmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated transformation of Jatropha curcas L. with a polyhydroxyalkanoate gene (phaC)

Novatiano

Pancoro

Marwani

2018

Indones. J. Biotechnol.

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Polyhydroxybutyrate is a component of bioplas cs that is synthesized under the control of enzymes encoded by pha mul genes. The genes are naturally present in Ralstonia eutropha. However, the produc on of bioplas cs in bacteria is inefficient because the bacterial biomass is rela vely small compared with plants or fungi. As such, engineering techniques have been developed that enable pha genes to be inserted into plant biomass, and then be expressed in the biomass of the plant to produce polyhydroxybutyrate. The objec ves of this study were to transform the ssue of Jatropha curcas using the phaC gene (a pha gene), to regenerate the transformed plant, and to confirm the presence of the inserted genes with PCR. The gene c transforma on of J. curcas was mediated by Agrobacterium tumefaciens strain GV3101 containing pARTC by dipping the cotyledon ssue of J. curcas in a suspension of the bacterium for 30 min, followed by cocul va on for 3 d on Murashige and Skoog (MS) medium. The ssue was then placed on a selec on medium, i.e. MS medium containing 13.3 µM BAP and 0.05 µM IBA with the addi on of 20 mg/L kanamycin. The results showed that 12.35% of the ssue survived and regenerated into a shoot a er 1-2 months. Molecular analysis of the transformed ssue was performed using phaC and nptII primers, in order to detect the presence of the phaC and nptII genes. Specific bands were detected at 659 bp and 700 bp, corresponding to the nptII primer and phaC primer, respec vely.

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“…For example in banana the triggering of apoptosis by A. tumefaciens can be effectively countered by the expression of antiapoptotic genes (Khanna et al 2007). While such a strategy has not been evaluated in sorghum, a heat shock pretreatment, which was previously reported to counter apoptosis in banana embryogenic callus, leading to improved transformation efficiency (Khanna et al 2004), has recently been shown to be a translatable technique using sorghum immature embryos (Gurel et al 2009). …”

Section: Sorghum Transformationmentioning

confidence: 99%