Sorghum Transformation: Overview and Utility

Kumar, Tejinder; Howe, Arlene; Sato, Shirley; Dweikat, İsmail; Clemente, Tom

doi:10.1007/978-1-4419-5947-8_10

Cited by 7 publications

(3 citation statements)

References 85 publications

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“…Agrobacterial transformation was based on previously published protocols [20,32] with some modifications. Immature embryos after pre-cultivation for 3 days on the agar M11 medium [33] were placed onto sterile filter paper wetted with inoculating medium and were inoculated…”

Section: °Cmentioning

confidence: 99%

“…Improving of sorghum genetic transformation technology [20,21] makes it possible to solve this problem by using RNA interference (RNAi) that allows targeted downregulation of individual genes. In recent years, RNAi technology has become widely used for changing the composition of the storage proteins and starch in different cereal species [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

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Development of Transgenic Sorghum Plants with Improved In Vitro Kafirin Digestibility

Elkonin¹,

Italyanskaya²,

Panin³

et al. 2017

Plant Engineering

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Improvement of nutritional value of crops is one of the main goals of plant biotechnology. These studies are extremely important for sorghum-a unique drought-tolerant cereal crop that is of special importance for sustainable grain production in the arid regions. The major cause of relatively low nutritive value of sorghum grain is the resistance of one of its seed storage proteins, γ-kafirin, to protease digestion. Using Agrobacteriummediated genetic transformation, we have obtained transgenic sorghum plants harboring a genetic construct for RNA interference (RNAi) silencing of the γ-kafirin gene. In T 1 generation, transgenic plants with modified endosperm texture were found. These plants had lowered level of the 28-kDa γ-kafirin protein and kafirin oligomers, which are formed by natural kafirin polymerization. In vitro protein digestibility analysis showed that the amount of undigested protein in transgenic plants was reduced by 2.9-3.2 times, in comparison with the original line, the digestibility index reached 85-88% (60% in the original line). HPLC analysis showed that total amino acid content in transgenic plants was reduced, while the lysine proportion was increased by 1.6-1.7 times. PCR analysis confirmed inheritance of the genetic construct up to T 4 generation.

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Section: °Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Transgenic Sorghum Plants with Improved In Vitro Kafirin Digestibility

Elkonin¹,

Italyanskaya²,

Panin³

et al. 2017

Plant Engineering

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“…Further introduction of sudangrass in the Lower Volga region depends on the creation of fun-damentally new varieties having high-yield and early mature, which are adapted to modern cultiva-tion technologies. The selection of sorghum crops, including sudangrass is focused on the use of the genetic function of the plant cytoplasm [8,9,10], heterosis [11,12], the study of the concentration and modification of kafirins [13]; protein modulation [14]; expression of apomictic potentials [15]; genetic modification as a tool to increase nutritional value [16,17,18].…”

Section: Introductionmentioning

confidence: 99%

Comparative assessment and scientific support of seed breeding of the new variety Evgeniya of sudangrass

Vertikova

Pylnev

2021

E3S Web Conf.

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The article characterizes the advantages of sudangrass, presents its comparative assessment, and gives recommendations for the cultivation of a new variety Evgeniya for seeds. Implementation of the program for the development of forage production in Russia and other countries implies not only the creation of new varieties but also the promotion of their introduction. This study aims to test the new variety Evgeniya of sudangrass and refine the cultivation techniques for obtaining high-quality seeds in the Volga region. For a period of 2 years, we studied the new promising variety, new lines that were obtained during interspecific, intraspecific, and intervarietal crossings carried out in different years, and new varieties of sudangrass recommended for cultivation in the region. The Yubileinaya 20 and Zonalskaya 6 varieties of sudangrass were used as standards. Field studies were carried out in 2017-2019. It was found that the new variety Evgeniya was characterized by high values of economically valuable traits. The variety had several biometric and biological features, such as thin stems and high yields, so we studied the influence of sowing methods and seeding rates not only on yield but on seed quality. To obtain the maximum yield of high-quality seeds, Evgeniya sudangrass must be sown with a seeding rate of 0.6-0.7 mln. viable seeds per hectare and row spacing of 30 cm.

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Bridging Conventional and Molecular Genetics of Sorghum Insect Resistance

Huang

Sharma

Dhillon

2012

Genomics of the Saccharinae

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Sustainable production of sorghum, Sorghum bicolor (L.) Moench, depends on effective control of insect pests as they continue to compete with humans for the sorghum crop. Insect pests are a major constraint in sorghum production, and nearly 150 insect species are serious pests of this crop worldwide and cause more than 9% loss annually. Annual losses due to insect pests in sorghum have been estimated to be $1,089 million in the semiarid tropics (ICRISAT Annual report 1991. International Crop Research Institute for Semi-arid Tropics. Patancheru, Andhra Pradesh, India, 1992), but differing in magnitude on a regional basis. Key insect pests in the USA include the greenbug, Schizaphis graminum (Rondani); sorghum midge, Stenodiplosis sorghicola (Coquillett); and various caterpillars in the Southern areas. For example, damage by greenbug to sorghum is estimated to cost US producers $248 million annually. The major insect pests of sorghum on a global basis are the greenbug, sorghum midge, sorghum shoot fl y (Atherigona soccata Rond.), stem borers (Chilo partellus Swin. and Busseola fusca Fuller), and armyworms (Mythimna separata Walk and Spodoptera frugiperda J.E. Smith). Recent advances Chapter 16

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Sorghum Transformation: Overview and Utility

Cited by 7 publications

References 85 publications

Development of Transgenic Sorghum Plants with Improved In Vitro Kafirin Digestibility

Development of Transgenic Sorghum Plants with Improved In Vitro Kafirin Digestibility

Comparative assessment and scientific support of seed breeding of the new variety Evgeniya of sudangrass

Bridging Conventional and Molecular Genetics of Sorghum Insect Resistance

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