Centrifugal Seeding Increases Seeding Efficiency and Cellular Distribution of Bone Marrow Stromal Cells in Porous Biodegradable Scaffolds

Abstract

Bone marrow stromal cells (MSCs) are a promising cell source for a variety of tissue engineering applications, given their ready availability and ability to differentiate into multiple cell lineages. MSCs have been successfully used to create neotissue for cardiovascular, urological, and orthopedic reconstructive surgical procedures in preclinical studies. The ability to optimize seeding techniques of MSCs onto tissue engineering scaffolds and the ability to control neotissue formation in vitro will be important for the rational design of future tissue engineering applications using MSCs. In this study we investigated the effect of centrifugal force on seeding MSCs into a biodegradable polyester scaffold. MSCs were isolated and seeded onto porous scaffold sections composed of nonwoven polyglycolic acid mesh coated with poly(L-lactide-co-epsilon-caprolactone). Compared to standard static seeding techniques, centrifugal seeding increased the seeding efficiency by 38% (p < 0.007) and significantly improved cellular distribution throughout the scaffold. Overall, centrifugal seeding of MSCs enhances seeding efficiency and improves cellular penetration into scaffolds, making it a potentially useful technique for manipulating neotissue formation by MSCs for tissue engineering applications.

Bio- And Hemocompatible Tissue-Engineered Polycaprolactone Scaffolds Seeded by Human Endothelial and Smooth Muscle Cells After Implantation in the Abdominal Aorta of Scid Mice

“…В соответствии с имеющимися данными, после имплантации протезов сосудов с заранее сформированным эндотелиальным слоем боль-шинство клеток открепляется от внутренней стенки в процессе операции или под действи-ем тока крови [43,44]. Оставшиеся клетки, тем не менее, своим паракринным эффектом могут предотвратить острый тромбоз трансплантатов в раннем послеоперационном периоде [45,46].…”

“…Narutoshi и др. предпо-лагают, что заселенные клетки погибают путем апоптоза [1], хотя еще одним фактором, умень-шающим количество прикрепеленных к поверх-ности полимерных матриксов клеток, является также и их механическое смывание [7,43,44]. В качестве каркаса для ТИС используют раз-личные материалы -как биологические [2,4], так и синтетические [3,5,6].…”

Cytocentrifugation: a convenient and efficient method for seeding tendon-derived cells into monolayer cultures or 3-D tissue engineering scaffolds

“…A number of approaches have been used to address this problem all with some degree of success. These include the use of magnetic particles (Shimizu et al 2006(Shimizu et al , 2007, perfusion (Dai et al 2009;Maidhof et al 2010), acoustic waves (Bok et al 2009;Li et al 2007) and, similar to the approach of this laboratory, centrifugation (Godbey et al 2004;Roh et al 2007;Ng et al 2010). All of these have demonstrated an improved ability to deliver cells to the centre of 3-D cell culture scaffolds and the choice of method would probably be dependent on other factors including cost, simplicity, selectivity and sterility.…”

Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration

“…This method is associated with a low seeding efficiency of approximately 10-25% (Roh et al, 2007) and a low rate of cell penetration inside the scaffold (Mo et al, 2004;Ravi et al, 2009;Ma, 2008). Even penetrated cells are unable to establish attachment with scaffold surfaces.…”