Centrifugal microfluidics for cell analysis

Burger, Robert; Kirby, Daniel P.; Glynn, Macdara; Nwankire, Charles; O’Sullivan, Mary C.; Siegrist, Jonathan; Kinahan, David J.; Aguirre, Gerson R.; Kijanka, Gregor; Gorkin, Robert; Ducrée, Jens

doi:10.1016/j.cbpa.2012.06.002

Cited by 89 publications

(61 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[10][11][12][13] Centrifugal microfluidics often utilize intrinsic, non-contact pumping and valving mechanisms which are ideal for liquid handling. [14][15][16] However, the handling of liquid/vapor PCR samples in microfluidic devices is not a simple task when confronted with the high temperatures and pressures created within the PCR chamber region.…”

Section: A Centrifugal Microfluidic Platformsmentioning

confidence: 99%

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

et al. 2013

View full text Add to dashboard Cite

A comprehensive study involving numerical analysis and experimental validation of temperature transients within a microchamber was performed for thermocycling operation in an integrated centrifugal microfluidic platform for polymerase chain reaction (PCR) amplification. Controlled heating and cooling of biological samples are essential processes in many sample preparation and detection steps for micrototal analysis systems. Specifically, the PCR process relies on highly controllable and uniform heating of nucleic acid samples for successful and efficient amplification. In these miniaturized systems, the heating process is often performed more rapidly, making the temperature control more difficult, and adding complexity to the integrated hardware system. To gain further insight into the complex temperature profiles within the PCR microchamber, numerical simulations using computational fluid dynamics and computational heat transfer were performed. The designed integrated centrifugal microfluidics platform utilizes thermoelectrics for ice-valving and thermocycling for PCR amplification. Embedded micro-thermocouples were used to record the static and dynamic thermal responses in the experiments. The data collected was subsequently used for computational validation of the numerical predictions for the system response during thermocycling, and these simulations were found to be in agreement with the experimental data to within $97%. When thermal contact resistance values were incorporated in the simulations, the numerical predictions were found to be in agreement with the experimental data to within $99.9%. This in-depth numerical modeling and experimental validation of a complex single-sided heating platform provide insights into hardware and system design for multi-layered polymer microfluidic systems. In addition, the biological capability along with the practical feasibility of the integrated system is demonstrated by successfully performing PCR amplification of a Group B Streptococcus gene. V C 2013 American Institute of Physics. [http://dx

show abstract

Section: A Centrifugal Microfluidic Platformsmentioning

confidence: 99%

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

et al. 2013

View full text Add to dashboard Cite

show abstract

“…This is the so-called "lab-on-a-disc" strategy. 14,15 In an endeavor to develop a compact, user-friendly, and cost-efficient sample-to-answer device for highly accurate target cell counts, we introduce here a novel method for centrifugo-magnetophoretic cell separation that is amenable to complete integration into a lab-on-a-disc platform. 14,16 As a proof-of-concept and an intermediate step, this work implements a bead-based immunomagnetic isolation of cells expressing the CD4 antigen on the cell surface-CD4-expressing cells being the primary host cell of the HIV virus and hence of prime diagnostic relevance.…”

Section: Introductionmentioning

confidence: 99%

Centrifugo-Magnetophoretic Purification of CD4+ Cells from Whole Blood Toward Future HIV/AIDS Point-of-Care Applications

et al. 2014

Self Cite

View full text Add to dashboard Cite

In medical diagnostics, detection of cells exhibiting specific phenotypes constitutes a paramount challenge. Detection technology must ensure efficient isolation of (often rare) targets while eliminating nontarget background cells. Technologies exist for such investigations, but many require high levels of expertise, expense, and multistep protocols. Increasing automation, miniaturization, and availability of such technologies is an aim of microfluidic lab-on-a-chip strategies. To this end, we present an integrated, dual-force cellular separation strategy using centrifugo-magnetophoresis. Whole blood spiked with target cells is incubated with (super-)paramagnetic microparticles that specifically bind phenotypic markers on target cells. Under rotation, all cells sediment into a chamber located opposite a co-rotating magnet. Unbound cells follow the radial vector, but under the additional attraction of the lateral magnetic field, bead-bound target cells are deflected to a designated reservoir. This multiforce separation is continuous and low loss. We demonstrate separation efficiently up to 92% for cells expressing the HIV/AIDS relevant epitope (CD4) from whole blood. Such highly selective separation systems may be deployed for accurate diagnostic cell isolations from biological samples such as blood. Furthermore, this high efficiency is delivered in a cheap and simple device, thus making it an attractive option for future deployment in resourcelimited settings.

show abstract

“…PCR) [7,8] and cell analysis [9,10]. One area where the centrifugal paradigm is particularly applicable is in pre-conditioning of whole blood.…”

Section: Introductionmentioning

confidence: 99%

Spira Mirabilis enhanced density gradient centrifguation

Kearney

Kinahan

Ducrée

2013

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS)

View full text Add to dashboard Cite

The centrifugal microfluidic "lab-on-a-disc" paradigm is particularly suited to the pre-conditioning of whole blood. In this work we define the geometry of sedimentation chambers using the Spira Mirabilis (equiangular spiral) and so enhance the Boycott Effect and increase the speed at which blood may be stratified using a density gradient media. We find that using the Spira Mirabilis defined chambers can increase the speed of stratification by up to 25% compared with equivalent linear chambers.

show abstract

Centrifugal microfluidics for cell analysis

Cited by 89 publications

References 35 publications

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

Experimental validation of numerical study on thermoelectric-based heating in an integrated centrifugal microfluidic platform for polymerase chain reaction amplification

Centrifugo-Magnetophoretic Purification of CD4+ Cells from Whole Blood Toward Future HIV/AIDS Point-of-Care Applications

Spira Mirabilis enhanced density gradient centrifguation

Contact Info

Product

Resources

About