Centrifugal microfluidic lab-on-a-chip system with automated sample lysis, DNA amplification and microarray hybridization for identification of enterohemorrhagic <i>Escherichia coli</i> culture isolates

Geißler, Matthias; Brassard, D.; Clime, Liviu; Pilar, Ana Victoria C.; Malic, Lidija; Daoud, Jamal; Barrère, Virginie; Luebbert, Christian; Blais, Burton W.; Corneau, Nathalie; Veres, Teodor

doi:10.1039/d0an01232g

Cited by 25 publications

(31 citation statements)

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“…Automated RT-LAMP molecular diagnostic assay was implemented on a cartridge operated by the centrifugal microfluidic platform which is described in detail elsewhere. [40][41][42][43] The platform is equipped with a programmable pumping and pressure control system that can apply regulated air pressure to two microfluidic cartridges located on opposite ends of the rotating stage. A movable pneumatic manifold lid is used to interface each cartridge and apply pressure (between −5 and +10 psi) from the pump to dedicated pressure inlet ports within the microfluidic circuit.…”

Section: Centrifugal Microfluidic Platform and Cartridgementioning

confidence: 99%

“…The platform is equipped with a programmable pumping and pressure control system to mediate fluid displacements on the cartridge. [40][41][42][43] The platform also features a stroboscopic imaging system for real-time visualization and recording of colorimetric signal, and thermoelectric elements integrated on the rotating stage for programmable, on-chip heating and cooling. The entire analytical workflow is conducted through a timed sequence of centrifugation and pneumatic actuation steps, which involve different operations such as downstream transfer, flow splitting, metering, inward pumping, and bubble-based mixing.…”

Section: Introductionmentioning

confidence: 99%

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Automated sample-to-answer centrifugal microfluidic system for rapid molecular diagnostics of SARS-CoV-2

et al. 2022

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Section: Centrifugal Microfluidic Platform and Cartridgementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Automated sample-to-answer centrifugal microfluidic system for rapid molecular diagnostics of SARS-CoV-2

et al. 2022

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“…Similarly, Geissler et al . established a microfluidic device for performing the whole process of bacteria identification for E. coli O157:H7 from cell lysis, multiplex PCR amplification, to on-chip hybridization with fluorescent gene markers [ 198 ]. More recently, Sullivan et al .…”

Section: Microfluidic Devices and Biosensors For Sars-cov-2 Diagnosticsmentioning

confidence: 99%

Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review

et al. 2021

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COVID-19 is one of the most severe global health crises that humanity has ever faced. Researchers have restlessly focused on developing solutions for monitoring and tracing the viral culprit, SARS-CoV-2, as vital steps to break the chain of infection. Even though biomedical engineering (BME) is considered a rising field of medical sciences, it has demonstrated its pivotal role in nurturing the maturation of COVID-19 diagnostic technologies. Within a very short period of time, BME research applied to COVID-19 diagnosis has advanced with ever-increasing knowledge and

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“… 23 , 25 These characteristics render Zeonor and other COP formulations attractive for fabricating polymer-based microfluidic systems for analytical, diagnostic, and life science applications. 23 , 26 − 29 We previously have shown that periodic arrays of micropillars produced in Zeonor can be used as templates for colorimetric DNA detection. 30 The approach takes advantage of higher surface-to-volume ratios that these arrays provide compared to planar substrates, making it possible to attach more probe molecules per surface area and so increase the signal obtained by the assay.…”

Section: Introductionmentioning

confidence: 99%

Use of Polymer Micropillar Arrays as Templates for Solid-Phase Immunoassays

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Ponton

Nassif

et al. 2022

ACS Appl. Polym. Mater.

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We investigate the use of periodic micropillar arrays produced by high-fidelity microfabrication with cyclic olefin polymers for solid-phase immunoassays. These three-dimensional (3D) templates offer higher surface-to-volume ratios than two-dimensional substrates, making it possible to attach more antibodies and so increase the signal obtained by the assay. Micropillar arrays also provide the capacity to induce wicking, which is used to distribute and confine antibodies on the surface with spatial control. Micropillar array substrates are modified by using oxygen plasma treatment, followed by grafting of (3-aminopropyl)triethoxysilane for binding proteins covalently using glutaraldehyde as a cross-linker. The relationship between microstructure and fluorescence signal was investigated through variation of pitch (10–50 μm), pillar diameter (5–40 μm), and pillar height (5–57 μm). Our findings suggest that signal intensity scales proportionally with the 3D surface area available for performing solid-phase immunoassays. A linear relationship between fluorescence intensity and microscale structure can be maintained even when the aspect ratio and pillar density both become very high, opening the possibility of tuning assay response by design such that desired signal intensity is obtained over a wide dynamic range compatible with different assays, analyte concentrations, and readout instruments. We demonstrate the versatility of the approach by performing the most common immunoassay formats—direct, indirect, and sandwich—in a qualitative fashion by using colorimetric and fluorescence-based detection for a number of clinically relevant protein markers, such as tumor necrosis factor alpha, interferon gamma (IFN-γ), and spike protein of severe acute respiratory syndrome coronavirus 2. We also show quantitative detection of IFN-γ in serum using a fluorescence-based sandwich immunoassay and calibrated samples with spike-in concentrations ranging from 50 pg/mL to 5 μg/mL, yielding an estimated limit of detection of ∼1 pg/mL for arrays with high micropillar density (11561 per mm 2 ) and aspect ratio (1:11.35).

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Centrifugal microfluidic lab-on-a-chip system with automated sample lysis, DNA amplification and microarray hybridization for identification of enterohemorrhagic Escherichia coli culture isolates

Abstract: The development of technology for the rapid, automated identification of bacterial culture isolates can help regulatory agencies to shorten response times in food safety surveillance, compliance, and enforcement as well...

Cited by 25 publications

References 69 publications

Automated sample-to-answer centrifugal microfluidic system for rapid molecular diagnostics of SARS-CoV-2

Automated sample-to-answer centrifugal microfluidic system for rapid molecular diagnostics of SARS-CoV-2

Recent findings and applications of biomedical engineering for COVID-19 diagnosis: a critical review

Use of Polymer Micropillar Arrays as Templates for Solid-Phase Immunoassays

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