Central nervous system effects of the essential oil of the leaves of &lt;I&gt;Alpinia zerumbet&lt;/I&gt; in mice

Araújo, Fernanda Yvelize Ramos de; Silva, Maria Izabel Gomes; Moura, Brinell Arcanjo; Oliveira, Gersilene Valente de; Leal, Luzia Kalyne Almeida Moreira; Vasconcelos, Silvânia Maria Mendes; Viana, Glauce Socorro Barros; Moraes, Manoel Odoríco de; Sousa, Francisca Cléa Florenço de; Macêdo, Danielle Silveira

doi:10.1211/jpp/61.11.0012

Cited by 14 publications

(14 citation statements)

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“…Studies have shown that essential oil of A. zerumbet (EOAz) has pharmacological effects; in particular, it lowers blood pressure by means of vascular smooth muscle relaxation (Pinto et al, 2009;Santos et al, 2011a;Lahlou et al, 2003), antioxidant (Elzaawely et al, 2007), anxiolytic (De Araújo et al, 2009) and fungistatic (Lima et al, 1993) activities.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

Cunha

Fechine

Bezerra

et al. 2016

Rev. bras. plantas med.

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ABSTRACT:The aim of this study was to characterize components of the EOAz and its hexane (HFEOAz), chloroform (CFEOAz) and methanol (MFEOAz) fractions, and its antihypertensive effect. EOAz was extracted from leaves by hydrodistillation. Aliquot was subjected to selective desorption with silica gel column and eluted with hexane, chloroform and methanol. The components of the EOAz and fractions were analyzed by gas chromatography coupled with mass spectrometry and nuclear magnetic resonance spectroscopy of hydrogen. Experiments of vascular reactivity were performed with isolated aortic rings of male Wistar rats. Antihypertensive effect was evaluated in hypertensive rats submitted to the inhibition of synthesis of nitric oxide. Blood pressure was measured indirectly by tail plethysmography. MFEOAz showed the lowest EC50 (150.45 µg/mL), 1,8-cineole (27.81%) and terpinen-4-ol (57.35%) as main components. Single administration by nasogastric tube of EOAz, fractions and captopril significantly reduced the blood pressure of hypertensive rats, when compared to animals of the negative control group with distilled water. In conclusion, the potency of the MFEOAz was higher than that of EOAz and other fractions. The antihypertensive effect of EOAz and fractions was similar, higher than the negative control and lower than that of captopril.Keywords: Alpinia, phytotherapy, vasodilation, hypertension.RESUMO: Estudo comparativo do efeito anti-hipertensivo das frações hexânixa, clorofórmica e metanólica do óleo essencial da Alpinia zerumbet. O objetivo deste estudo foi caracterizar os componentes do óleo essencial das folhas de Alpinia zerumbet (OEAz) e suas frações hexânica (FHOEAz), clorofórmica (FCOEAz) e metanólica (FMOEAz), e seu efeito anti-hipertensivo. OEAz foi extraído das folhas por hidrodestilação. Uma alíquota foi submetida à desadsorção seletiva com coluna de gel de sílica e eluída com hexano, clorofórmio e metanol. Os componentes do OEAz e fracções foram analisadas por cromatografia gasosa acoplada à detector de massa e por espectros de ressonância magnética nuclear de hidrogênio. Experimentos de reatividade vascular foram realizados com anéis aórticos isolados de ratos Wistar machos. Efeito anti-hipertensivo foi avaliado em ratos hipertensos submetidos à inibição da síntese de óxido nítrico. A pressão arterial foi medida indiretamente por pletismografia de cauda. FMOEAz mostrou a menor CE50 (150,45 μg/mL), 1,8-cineol (27,81%) e terpinen-4-ol (57,35%) como componentes principais. A administração em dose única por sonda nasogástrica de OEAz, frações e captopril reduziu significativamente a pressão arterial de ratos hipertensos, quando comparados aos animais do grupo controle negativo com água destilada. Em conclusão, a potência da FMOEAz foi maior que a do OEAz e outras frações. O efeito anti-hipertensivo de OEAz e frações foi semelhante, maior do que o controle negativo e menor do que o captopril.Palavras-chave: Alpinia, fitoterapia, vasodilação, hipertensão.

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Section: Introductionmentioning

confidence: 99%

Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

Cunha

Fechine

Bezerra

et al. 2016

Rev. bras. plantas med.

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“…We employed two well-validated models which explore the behavior of mice in novel environments, namely the open-field test (OF) and the light–dark choice test (LD), to acquire various behavioral parameters used in neuropsychopharmacology for drug screening. The OF test (Walsh and Cummins 1976; Simon et al 1994; Prut and Belzung 2003; Lamprea et al 2008) is widely used as a test of locomotion since the mice are free to move in the entire field, and the distance traveled during a fixed time can be measured (Mi et al 2005; de Araujo et al 2009; Vasconcelos et al 2009). However, a detailed ethological analysis of the OF test, including time spent and distance traveled in the center/periphery of the field, makes it possible not only to examine general activity but also to detect specific effects of drugs on behavioral parameters such as field exploration propensity and thigmotaxis (Choleris et al 2001; Ramos et al 2008; Fukushiro et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The two-compartment LD model system (Crawley and Goodwin 1980; Hascoet et al 2001; Belzung and Griebel 2001; Bourin et al 2007) is one of the most commonly used behavioral paradigms: classic anxiolytics (benzodiazepines) as well as the newer anxiolytic-like compounds (e.g. serotonergic drugs or drugs acting on neuropeptide receptors) (Bourin and Hascoet 2003) and natural compounds (Chen et al 2004; de Araujo et al 2009) can be detected using this paradigm, since they specifically increase the time spent in the lit compartment and the number of exploratory transitions.…”

Section: Introductionmentioning

confidence: 99%

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

et al. 2010

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IntroductionThis study was designed to investigate the putative anxiolytic-like activity of ultra-low doses of Gelsemium sempervirens (G. sempervirens), produced according to the homeopathic pharmacopeia.MethodsFive different centesimal (C) dilutions of G. sempervirens (4C, 5C, 7C, 9C and 30C), the drug buspirone (5 mg/kg) and solvent vehicle were delivered intraperitoneally to groups of ICR-CD1 mice over a period of 9 days. The behavioral effects were assessed in the open-field (OF) and light–dark (LD) tests in blind and randomized fashion.ResultsMost G. sempervirens dilutions did not affect the total distance traveled in the OF (only the 5C had an almost significant stimulatory effect on this parameter), indicating that the medicine caused no sedation effects or unspecific changes in locomotor activity. In the same test, buspirone induced a slight but statistically significant decrease in locomotion. G. sempervirens showed little stimulatory activity on the time spent and distance traveled in the central zone of the OF, but this effect was not statistically significant. In the LD test, G. sempervirens increased the % time spent in the light compartment, an indicator of anxiolytic-like activity, with a statistically significant effect using the 5C, 9C and 30C dilutions. These effects were comparable to those of buspirone. The number of transitions between the compartments of the LD test markedly increased with G. sempervirens 5C, 9C and 30C dilutions.ConclusionThe overall pattern of results provides evidence that G. sempervirens acts on the emotional reactivity of mice, and that its anxiolytic-like effects are apparent, with a non-linear relationship, even at high dilutions.

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“…Previous studies indicate that inward currents in motoneurons are mediated by L-type calcium channel [33]. Here, the assumption of a central Ca 2+ -mediated action finds support because the systemic administration of EOAz triggers effects on the central nervous system [34,35]. In addition, the vasorelaxant and antihypertensive effects of a methanolic fraction of EOAz (MFEOAz) were related to an inhibition of the calcium influx via voltage-operated calcium channels, receptor-operated calcium channels and L-type calcium channels [3].…”

Section: Discussionmentioning

confidence: 82%

The Effect of Alpinia zerumbet Essential Oil on Post‐Stroke Muscle Spasticity

Maia

Dantas

Filho

et al. 2015

Basic Clin Pharma Tox

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The essential oil of Alpinia zerumbet (EOAz) presents myorelaxant and antispasmodic actions on cardiac and smooth muscles. The aim of this study was to investigate the effect of EOAz on the skeletal muscle contraction in post-stroke spasticity. Fifteen adults with unilateral hemiparesis and spasticity resulting from stroke were submitted to surface electromyography readings of the gastrocnemius muscle, before and after 10 daily applications (dermal 0.05 mL per muscle belly) of EOAz. The healthy contralateral muscles without applying the oil were used as controls. The analysis showed that, in both lateral and medial gastrocnemius, the values of all studied variables (root mean square, maximum amplitude and median power frequency) were significantly decreased in pathological legs during muscle contraction (Wilcoxon test, p < 0.05). Moreover, spastic muscles presented different results before and after dermal application of EOAz: The mean values of root mean square and median power frequency were significantly increased in lateral and medial gastrocnemius, and also, the maximum amplitude increased in medial gastrocnemius (Mann-Whitney test, p < 0.05). The results suggest that EOAz acts in the skeletal spastic muscle contraction by promoting relaxation and improvement of the muscular performance. Thus, the EOAz can be useful for the clinical management of secondary effects in patients with cerebral vascular disease.The Alpinia speciosa K. Schum., synonymy zerumbet, is a plant of Asian origin and member of the Zingiberaceae family [1,2]. It is widely distributed in Brazil, where it is popularly called 'colony'. The essential oil and extracts obtained from aerial parts of Alpinia zerumbet present antihypertensive [3][4][5][6], antioxidant [7,8] and anxiolytic [9] activities, among others.Some actions of essential oil of Alpinia zerumbet (EOAz) and its main constituents have been reported on the nervous and muscular systems. In experimental studies, the EOAz presented anticholinergic, antispasmodic and myorelaxant effects on small intestine of rodents [10,11]; promoted a dose-dependent blockade of the compound action potential of the rat sciatic nerve [12]; and presented endothelium-dependent vasorelaxant effect [4,13]. Also, the oil induced a cardiodepressive effect in vitro, this action being related to calcium current blockade [14]. These findings raise the possibility that the EOAz can act on altered contractile properties of skeletal fibres.In stroke patients, spasticity occurs as a result of spinal reflex dysfunction and is clinically related to abnormal increased muscle tone [15] and consequent impaired muscular performance [16]. This illness causes disabilities in more than 50% of affected people and impacts family and healthcare systems [17]. The current treatments are not fully effective, and new therapeutic strategies are desirable.From the hypothesis that EOAz could be a complementary medicine to treat spasticity by presenting relaxant action, this work aimed to investigate the effects of the derm...

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Central nervous system effects of the essential oil of the leaves of <I>Alpinia zerumbet</I> in mice

Abstract: The essential oil of A. zerumbet leaves had depressant and possible antipsychotic activity, since it could reverse the stereotypy induced by apomorphine, presenting effects comparable with those obtained with haloperidol treatment.

Cited by 14 publications

References 29 publications

Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

Comparative study of the antihypertensive effects of hexane, chloroform and methanol fractions of essential oil of Alpinia zerumbet in rats Wistar

Dose-effect study of Gelsemium sempervirens in high dilutions on anxiety-related responses in mice

The Effect of Alpinia zerumbet Essential Oil on Post‐Stroke Muscle Spasticity

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