Central connections of <i>Limulus</i> ventral photoreceptors revealed by intracellular staining

Batra, Ranjan; Chamberlain, Steven C.

doi:10.1002/neu.480160604

Cited by 8 publications

(1 citation statement)

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“…Aspects that have been explored in the past include adult ommatidial morphology of the lateral eyes (review Fahrenbach, 1975), organization of the retinal projections into the brain as well as efferent projections Barlow, 1980, 1982;Batra and Chamberlain, 1985;, and distribution of the visual pathway neurotransmitters (reviewed in Battelle et al, 1999) octopamine (Evans et al, 1983), serotonin , FMRFamide and substance P (Chamberlain and Engbretson, 1982;Lewandoswski et al, 1989), histamine , and acetylcholine (Hornstein et al, 1994). Additionally, circadian modulation of the photoresponse (e.g., Battelle et al, 1998Battelle et al, , 2000Battelle et al, , 2001Sacunas et al, 2002;Waren and Chamberlain, 2002;Ankrom and Chamberlain, 2002;Dalal et al, 2003;Pieprzyk et al, 2003;Runyon et al, 2004;reviews, Barlow, 2001;Battelle, 2002), and both computational and behavioral aspects of vision (reviewed in Barlow et al, 2001) are active areas of current research.…”

Section: Introductionmentioning

confidence: 99%

Evolution of arthropod visual systems: Development of the eyes and central visual pathways in the horseshoe crabLimulus polyphemusLinnaeus, 1758 (Chelicerata, Xiphosura)

Harzsch

Vilpoux

Blackburn

et al. 2006

Developmental Dynamics

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Despite ongoing interest into the architecture, biochemistry, and physiology of the visual systems of the xiphosuran Limulus polyphemus, their ontogenetic aspects have received little attention. Thus, we explored the development of the lateral eyes and associated neuropils in late embryos and larvae of these animals. The first external evidence of the lateral eyes was the appearance of white pigment spots-guanophores associated with the rudimentary photoreceptors-on the dorsolateral side of the late embryos, suggesting that these embryos can perceive light. The first brown pigment emerges in the eyes during the last (third) embryonic molt to the trilobite stage. However, ommatidia develop from this field of pigment toward the end of the larval trilobite stage so that the young larvae at hatching do not have object recognition. Double staining with the proliferation marker bromodeoxyuridine (BrdU) and an antibody against L. polyphemus myosin III, which is concentrated in photoreceptors of this species, confirmed previous reports that, in the trilobite larvae, new cellular material is added to the eye field from an anteriorly located proliferation zone. Pulse-chase experiments indicated that these new cells differentiate into new ommatidia. Examining larval eyes labeled for opsin showed that the new ommatidia become organized into irregular rows that give the eye field a triangular appearance. Within the eye field, the ommatidia are arranged in an imperfect hexagonal array. Myosin III immunoreactivity in trilobite larvae also revealed the architecture of the central visual pathways associated with the median eye complex and the lateral eyes. Double labeling with myosin III and BrdU showed that neurogenesis persists in the larval brain and suggested that new neurons of both the lamina and the medulla originate from a single common proliferation zone. These data are compared with eye development in Drosophila melanogaster and are discussed with regard to new ideas on eye evolution in the Euarthropoda. Developmental Dynamics 235:2641-2655, 2006.

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Section: Introductionmentioning

confidence: 99%

Evolution of arthropod visual systems: Development of the eyes and central visual pathways in the horseshoe crabLimulus polyphemusLinnaeus, 1758 (Chelicerata, Xiphosura)

Harzsch

Vilpoux

Blackburn

et al. 2006

Developmental Dynamics

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Central projections of Limulus photoreceptor cells revealed by a photoreceptor‐specific monoclonal antibody

Calman

Lauerman

Andrews

et al. 1991

J of Comparative Neurology

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Studies of lateral, median, and ventral eyes of the chelicerate arthropod Limulus polyphemus (the common American horseshoe crab) are providing important basic information about mechanisms for information processing in the peripheral visual system and for the modulation of visual responses by light and circadian rhythms. The processing of visual information in Limulus brain is less well understood in part because the specific central projections of the various classes of visual neurons are not known. This study describes a mouse monoclonal antibody, 3C6A3, which binds to Limulus photoreceptor cell bodies, their axons, and terminals, but not to any other cell type in the central nervous system. This antibody, and intracellular injection of biocytin, are used to demonstrate the central projections of each type of photoreceptor. Our main conclusions are that: 1) the photoreceptors (retinular cells) of the lateral eye project only to the lamina; 2) the photoreceptors of the lateral rudimentary eye project to both the lamina and medulla; 3) the photoreceptors of the median ocellus project only to the ocellar ganglion; and 4) the photoreceptors of the rudimentary median (endoparietal) eye project to the ocellar ganglion and also into the optic tract. These results, along with previous studies, allow us to infer the projections of the secondary cells. The eccentric cells of the lateral eye project to the lamina, medulla, optic tract, and ocellar ganglion. The arhabdomeral cells of the median ocellus project through the ocellar ganglion and to optic tract to the medulla.

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An atlas of the brain of the horseshoe crab Limulus polyphemus

Chamberlain

Wyse

1986

Journal of Morphology

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An atlas of the brain of the horseshoe crab Limulus polyphemus is developed. All of the neuronal groups are identified and named, and regions of neuropil are segregated and named where possible. The nomenclature incorporates functionally neutral earlier names and assigns geographical names to newly distinguished structures. The atlas provides a basis for correlating the results of neuroanatomical, neurophysiological, and neurochemical studies, which yield information about individual neurons or groups of neurons in this species.

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Central connections of Limulus ventral photoreceptors revealed by intracellular staining

Cited by 8 publications

References 20 publications

Evolution of arthropod visual systems: Development of the eyes and central visual pathways in the horseshoe crabLimulus polyphemusLinnaeus, 1758 (Chelicerata, Xiphosura)

Evolution of arthropod visual systems: Development of the eyes and central visual pathways in the horseshoe crabLimulus polyphemusLinnaeus, 1758 (Chelicerata, Xiphosura)

Central projections of Limulus photoreceptor cells revealed by a photoreceptor‐specific monoclonal antibody

An atlas of the brain of the horseshoe crab Limulus polyphemus

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