Cenp-F gene amplification and overexpression in head and neck squamous cell carcinomas

Guardia, Carola de la; Casiano, Carlos A.; Trinidad‐Pinedo, Juan; Báez, Adriana

doi:10.1002/1097-0347(200102)23:2<104::aid-hed1005>3.0.co;2-0

Cited by 47 publications

(27 citation statements)

References 36 publications

(53 reference statements)

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“…32 CENPF amplification and overexpression were additionally detected in a subset of head and neck squamous carcinomas. 33 However, we did not find any amplification of these genes in our series of malignant gliomas.…”

Section: Figure 2 -Demonstration Of Cntn2 Mdm4 Pepp3 and Sox13contrasting

confidence: 63%

Refined mapping of 1q32 amplicons in malignant gliomas confirms MDM4 as the main amplification target

Riemenschneider

Knobbe

Reifenberger

2003

Intl Journal of Cancer

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To address the question of whether 1q32 carries 2 independent amplification targets or a common target other than MDM4 and CNTN2, we analyzed primary malignant gliomas for amplification and overexpression of 17 different genes from this region. Our results indicate a single region of amplification that comprises the genes MDM4, GAC1, PIK3C2B and PEPP3, with only MDM4 amplification being invariably associated with overexpression. CNTN2 was found to be coamplified with MDM4 in 3 malignant gliomas but overexpressed in only 1 of these tumors. No CNTN2 amplification was detected in any of 102 malignant gliomas without MDM4 amplification. Our data therefore corroborate the notion that MDM4 is the main amplification target on 1q32 in malignant gliomas. However, coamplification and overexpression of adjacent genes may provide an additional growth advantage in some malignant gliomas with MDM4 amplification.

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Section: Figure 2 -Demonstration Of Cntn2 Mdm4 Pepp3 and Sox13contrasting

confidence: 63%

Refined mapping of 1q32 amplicons in malignant gliomas confirms MDM4 as the main amplification target

Riemenschneider

Knobbe

Reifenberger

2003

Intl Journal of Cancer

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“…More and more kinetochore proteins were proved to be associated with carcinogenesis. CENP-F and INCENP are upregulated in human cancer cells (38,39). CENP-A has been shown to be overexpressed in colorectal cancer cells and is mistargeted to noncentromeric regions (22).…”

Section: Discussionmentioning

confidence: 99%

Centromere Protein H Is a Novel Prognostic Marker for Nasopharyngeal Carcinoma Progression and Overall Patient Survival

Liao¹,

Song²,

Zhang

et al. 2007

Clinical Cancer Research

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Purpose: The aim of the present study was to analyze the expression of Centromere protein H (CENP-H), one of the fundamental components of the human active kinetochore, in nasopharyngeal carcinoma (NPC) and to correlate it with clinicopathologic data, including patient survival. Experimental Design: Using reverse transcription-PCR and Western blot, we detected the expression of CENP-H in normal nasopharyngeal epithelial cells, immortalized nasopharyngeal epithelial cell lines, and NPC cell lines. Using immunohistochemistry, we analyzed CENP-H protein expression in160 clinicopathologically characterized NPC cases. Statistical analyses were applied to test for prognostic and diagnostic associations. Results: Reverse transcription-PCR and Western blot showed that the expression level of CENP-H was higher in NPC cell lines and in immortalized nasopharyngeal epithelial cells than in the normal nasopharyngeal epithelial cell line at both transcriptional and translational levels. By immunohistochemical analysis, we found that 76 of 160 (47.5%) paraffin-embedded archival NPC biopsies showed high expression of CENP-H. Statistical analysis showed that there was a significant difference of CENP-H expression in patients categorized according to clinical stage (P = 0.024) and Tclassification (P = 0.027). Patients with higher CENP-H expression had shorter overall survival time, whereas patients with lower CENP-H expression had better survival. A prognostic value of CENP-H was also found of the subgroup of N 0 -N 1 tumor classification. Multivariate analysis showed that CENP-H expression was an independent prognostic indicator for patient's survival. Conclusions: Our results suggest that CENP-H protein is a valuable marker of NPC progression. High CENP-H expression is associated with poor overall survival in NPC patients.

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“…Kinetochore proteins, such as CENP-F, CENP-A, and Nek2 kinase, are overexpressed in tumors (25 -28) with the CENP-F locus being amplified in head and neck tumors (29). The aurora kinases have been implicated in neoplasia and have altered expression in tumor progression (30) as do key elements of the cytokinesis apparatus, such as citron kinase (31,32) and polo-like kinases (33).…”

Section: Discussionmentioning

confidence: 99%

The Septin-Binding Protein Anillin Is Overexpressed in Diverse Human Tumors

Hall

Todd

Hyland

et al. 2005

Clinical Cancer Research

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Anillin is an actin-binding protein that can bind septins and is a component of the cytokinetic ring.We assessed the anillin expression in 7,579 human tissue samples and cell lines by DNA microarray analysis. Anillin is expressed ubiquitously but with variable levels of expression, being highest in the central nervous system. The median level of anillin mRNA expression was higher in tumors than normal tissues (median fold increase 2.58; 95% confidence intervals, 2.19-5.68, P < 0.0001) except in the central nervous system where anillin mRNA levels were lower in tumors.We developed a sensitive reverse transcription-PCR strategy to show that anillin mRNA is expressed in cell lines and in cDNA panels derived from fetal and adult tissues, thus validating the microarray data. We compared anillin with Ki67 mRNA expression and found a significant linear relationship between anillin and Ki67 mRNA expression (Spearmann r f 0.6, P < 0.0001). Anillin mRNA expression was analyzed during tumor progression in breast, ovarian, kidney, colorectal, hepatic, lung, endometrial, and pancreatic tumors and in all tissues there was progressive increase in anillin mRNA expression from normal to benign to malignant to metastatic disease. Finally, we used anti-anillin sera and found nuclear anillin immunoreactivity to be widespread in normal tissues, often not correlating with proliferative compartments. These data provide insight into the existence of nonproliferation-associated activities of anillin and roles in interphase nuclei. Thus, anillin is overexpressed in diverse common human tumors, but not simply as a consequence of being a proliferation marker. Anillin may have potential as a novel biomarker.

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Cenp-F gene amplification and overexpression in head and neck squamous cell carcinomas

Cited by 47 publications

References 36 publications

Refined mapping of 1q32 amplicons in malignant gliomas confirms MDM4 as the main amplification target

Refined mapping of 1q32 amplicons in malignant gliomas confirms MDM4 as the main amplification target

Centromere Protein H Is a Novel Prognostic Marker for Nasopharyngeal Carcinoma Progression and Overall Patient Survival

The Septin-Binding Protein Anillin Is Overexpressed in Diverse Human Tumors

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