Cementum matrix formation in vivo by cultured dental follicle cells

Handa, Keisuke; Saito, Masahiro; Yamauchi, Masato; Kiyono, Tohru; Sato, Sunao; Teranaka, Toshio; Narayanan, A. Sampath

doi:10.1016/s8756-3282(02)00868-2

Cited by 121 publications

(120 citation statements)

References 40 publications

Supporting

Mentioning

115

Contrasting

Unclassified

Order By: Relevance

“…(7,8) Moreover, Zhao et al found that bone morphogenic protein-2 promotes differentiation of the dental follicle cells into the cementoblast/ osteoblast lineage, (9) and we have previously shown that bovine dental follicle cells (BDFCs) isolated from developing tooth germ are able to form cementum matrix when transplanted into immunodeficient mice. (10) In a previous study, the cementum matrix formed by BDFCs was heavily stained after addition of anti-cementum-specific monoclonal antibody (mAb 3G9). This antibody was raised against partially purified bovine cementum matrix containing cementum-derived attachment protein and specifically recognizes cementum matrix and cementoblasts but not tissues such as bone matrix.…”

Section: Introductionmentioning

confidence: 92%

“…(10) Briefly, bovine dental follicle tissue was removed from the root dentin of developing tooth germs with a scalpel and digested with 3 mg/ml bacterial collagenase (Boehringer Mannheim, Mannheim, Germany) in Krebs buffer (111.2 mM NaCl, 21.3 mM Tris, 13.0 mM glucose, 5.4 mM KCl, 1.3 mM MgCl 2 and 1.5 mM ZnCl 2 ). The released cells were incubated with ␣-MEM (Life Technologies) containing 10% FBS (BioWhittaker, Walkersville, MD, USA), 50 g/ ml of ascorbic acid, and 100 units/ml of streptomycin and penicillin in a humidified atmosphere of 5% CO 2 at 37°C.…”

Section: Tissue Culturementioning

confidence: 99%

“…(10) Briefly, cell clones were inoculated subcutaneously into 5-week-old female CB-17 scid/scid (SCID; severe combined immunodeficiency) mice (Nihoncrea, Tokyo, Japan) after incubating 1.5 × 10 6 cells in a mixture of 40 mg of hydroxyapatite powder (Apaseram, Pentax, Tokyo, Japan) and fibrin clot (mixture of mouse fibrinogen and thrombin: both from Sigma, St Louis, MO, USA). Mice were killed after 4 weeks, and implants were cut in half for histochemical analysis and analysis of mRNA expression of cementum matrix components.…”

Section: In Vivo Differentiation Assaymentioning

confidence: 99%

“…To avoid nonspecific staining of mouse monoclonal antibodies, sections were blocked using the M.O.M kit (Vector Burlingame) as described previously. (10) The sections were incubated with mAb 3G9 (5 g/ml) for 1 h and treated with biotinylated secondary antibody and avidin-peroxidase conjugate. Dilutions were made with PBS containing 1 mg/ml bovine serum albumin.…”

Section: Histochemical Analysismentioning

confidence: 99%

See 3 more Smart Citations

Immortalization of Cementoblast Progenitor Cells With Bmi-1 and TERT

Saito

Handa

Kiyono³

et al. 2005

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

A cementoblast progenitor cell line designated BCPb8 was successfully isolated from dental follicle cells immortalized with Bmi-1 and hTERT. BCPb8 showed the potential to differentiate into cementoblasts on implantation into immunodeficient mice. BCPb8 was confirmed to be the first established cementoblast progenitor cell line and will provide a useful model for investigating cementogenesis. Introduction:The dental follicle is the mesenchymal tissue surrounding the developing tooth germ. During tooth root development, progenitor cells present in the dental follicle are believed to play a central role in the formation of periodontal components (cementum, periodontal ligament, and alveolar bone). However, little more is known about the biology of these progenitors. Previously, we observed that cultured bovine dental follicle cells (BDFCs) contained putative cementoblast progenitors. To further analyze the biology of these cells, we attempted to isolate cementoblast progenitors from immortalized BDFC through expression of the polycomb group protein, Bmi-1, and human telomerase reverse transcriptase (hTERT). Materials and Methods: BDFCs were transduced with replication-deficient retroviruses carrying human Bmi-1(LXSN-Bmi-1), and hTERT (LXSH-hTERT) for immortalization. Single cell clones were established from immortalized BDFC, and differentiation into cementoblasts was assessed by implantation into immunodeficient mice. Results and Conclusion: BDFCs expressing Bmi-1 and hTERT showed an extended life span-90 population doublings more than normal BDFCs-and still contained cells with the potential to differentiate into cementoblasts on implantation into immunodeficient mice. From these cells, we established a clonal cell line, designated BCPb8, which formed cementum-like tissue that was reactive to the anti-cementum-specific monoclonal antibody 3G9 and expressed mRNA for bone sialoprotein, osteocalcin, osteopontin, and type I collagen on implantation. Thus, by using Bmi-1 and hTERT, we succeeded in immortalizing cementoblast progenitor cells from BDFC without affecting differentiation potential. The BCPb8 cell line is the first immortalized clonal cell line of cementoblast progenitors and could be a useful tool not only to study cementogenesis but also to develop regeneration therapy for patients with periodontitis.

show abstract

Section: Introductionmentioning

confidence: 92%

Section: Tissue Culturementioning

confidence: 99%

Section: In Vivo Differentiation Assaymentioning

confidence: 99%

Section: Histochemical Analysismentioning

confidence: 99%

See 2 more Smart Citations

Immortalization of Cementoblast Progenitor Cells With Bmi-1 and TERT

Saito

Handa

Kiyono³

et al. 2005

Journal of Bone and Mineral Research

View full text Add to dashboard Cite

show abstract

“…Dental pulp tissues were obtained from 12 weeksold male Wister rat (Hokudo, Sapporo, Japan) lower incisor, according to a procedure of Handa et al 22) . Their tissues were digested sequentially 4 times with 3 mg/ mL of bacterial collagenase (Roche Diagnostics, Basel, Switzerland) in phosphate-buffered saline.…”

Section: Tissue Culturementioning

confidence: 99%

The possibility of genistein as a new direct pulp capping agent

et al. 2013

View full text Add to dashboard Cite

Genistein, kind of soy isoflavones, is well-known as natural ingredients and consumed as health foods and supplements. They are expected to improve renal function. They have high-affinity to estrogen receptor β expressed predominantly in bone tissue, they prevent osteoporosis specifically and safely. We examined whether genistein can be a new direct capping agent. In this study, we examined the effect of genistein for the proliferation and differentiation of rat dental pulp cells in vitro and the ability of tertiary dentin formation in vivo. As a result, rat dental pulp cells with genistein were increased activity of ALPase and showed alizarin red positive-staining. Calcification-related genes expression has been confirmed by the addition of genistein. From in vivo study, high quality of tertiary dentin formation and minor pulp reaction were observed. From these findings, it was suggested that genistein may be useful agent for direct pulp capping.

show abstract

The Crowning Achievement: Getting to the Root of the Problem

Fong

Foster

Popowics

et al. 2005

Journal of Dental Education

View full text Add to dashboard Cite

An ideal goal of oral-craniofacial dental reconstructive therapy is to establish treatment modalities that predictably restore functional tissues. One major area of focus has been in the area of dental materials with marked improvements in the design of materials used to restore teeth/periodontium/bone lost as a consequence of disease or disorders. With advances in understanding the cell and molecular controls for development and regeneration of tooth structures, it is now possible to consider therapies that promote regeneration of lost tissues, along with replacement of these tissues. This review presents a background on our current knowledge as to the composition of the tooth/periodontium followed by a discussion on successes to date, both in vitro and in vivo, toward regenerating a whole tooth and next steps required to regenerate a functional tooth.

show abstract

Cementum matrix formation in vivo by cultured dental follicle cells

Cited by 121 publications

References 40 publications

Immortalization of Cementoblast Progenitor Cells With Bmi-1 and TERT

Immortalization of Cementoblast Progenitor Cells With Bmi-1 and TERT

The possibility of genistein as a new direct pulp capping agent

The Crowning Achievement: Getting to the Root of the Problem

Contact Info

Product

Resources

About