Cementum engineering with three‐dimensional polymer scaffolds

Abstract

Cloned cementoblasts (OCCMs), periodontal ligament fibroblasts (SV-PDLs), and dental follicle (SV-F) cells obtained from mice were used as a tool to study periodontal tissue engineering. OCCM, SV-PDL, and SV-F cells were seeded onto three-dimensional poly lactic-co-glycolic acid (PLGA) scaffolds and cultured with the use of bioreactors or implanted subcutaneously in severe combined immune deficiency (SCID) mice for up to 6 weeks. We explored the behavior of these cells in porous PLGA sponges by cell growth, expression of mineral-associated genes using reverse transcriptase polymerase chain reaction, and mineralization by histologic analysis in vitro and in vivo. Results indicated that cells attached to PLGA scaffolds under either static or dynamic conditions in vitro. Only OCCM implants, retrieved from both in vitro bioreactors and SCID mice at 3-and 6-weeks post-cell implantation exhibited mineral formation. Types I and XII collagens, osteocalcin, and bone sialoprotein genes were detected in all implants retrieved from SCID mice. These results suggest that delivery of selected cells via PLGA scaffolds may serve as a viable approach for promoting periodontal tissue regeneration.

Effect of Sustained Gene Delivery of Platelet‐Derived Growth Factor or Its Antagonist (PDGF‐1308) on Tissue‐Engineered Cementum

“…1B). The surgical procedure has been described previously in detail by Jin et al 29 The management of the animals was performed in accordance with a protocol approved by the Committee on the Care and Use of Animals at the University of Michigan. Four blocks of cell-implants were placed in the dorsum of each animal.…”

“…Percentage of mineral to total tissue (density) of the implants was determined by dividing area of mineralization by total area of the specimen as previously described. 29 In addition, the number of multinucleated giant cells (MNGCs) was counted in each specimen at 3 and 6 weeks. Cell density was determined by dividing cell number by the total tissue area for each specimen.…”

Noggin Gene Delivery Inhibits Cementoblast-Induced Mineralization

“…The procedures for seeding and implantation have been previously reported in detail [20]. In brief, 1 million OCCM cells were transduced in vitro at an MOI of 100 with one of three adenoviruses as above (Ad-GFP, Ad-NOGΔB2, or Ad-BMP-7) or without adenovirus transduction (NT).…”

Adhesion of human periodontal ligament cells by three-dimensional culture to the sterilized root surface of extracted human teeth

“…Abs = absorbance cells derived from adipose tissues (27). Several studies have investigated the development of scaffolds using biomaterials for periodontal tissue regeneration (28)(29)(30)(31). However, none of these scaffolds were able to reproduce the complex structure and function of the PDL.…”