Effect of Sustained Gene Delivery of Platelet‐Derived Growth Factor or Its Antagonist (PDGF‐1308) on Tissue‐Engineered Cementum

Anusaksathien, Orasa; Jin, Qiming; Zhao, Ming; Somerman, Martha J.; Giannobile, William V.

doi:10.1902/jop.2004.75.3.429

Cited by 59 publications

(38 citation statements)

References 54 publications

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“…Surgically created alveolar bone wounds treated with AdPDGF-B revealed significant regeneration of cementum and PDL, along with extended growth factor expression, as demonstrated by in vivo biodistribution (Jin et al, 2004a). Application of a dominant-negative mutant of the PDGF-A gene (PDGF-1308) directly to periodontal osseous defects or to cementoblasts transplanted ex vivo in polymer scaffolds led to impaired periodontal regeneration (Anusaksathien et al, 2004;Jin et al, 2004b). BMP viral gene delivery has showed remarkable potential in the regeneration of both long bones and craniofacial bones (Lieberman et al, 1998(Lieberman et al, , 1999Alden et al, 2000;Baltzer et al, 2000;Krebsbach et al, 2000;Shen et al, 2004a, b;Dunn et al, 2005).…”

Section: (43) Gene Delivery In Periodontal Tissue Engineeringmentioning

confidence: 99%

Craniofacial Tissue Engineering by Stem Cells

et al. 2006

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Craniofacial tissue engineering promises the regeneration or de novo formation of dental, oral, and craniofacial structures lost to congenital anomalies, trauma, and diseases. Virtually all craniofacial structures are derivatives of mesenchymal cells. Mesenchymal stem cells are the offspring of mesenchymal cells following asymmetrical division, and reside in various craniofacial structures in the adult. Cells with characteristics of adult stem cells have been isolated from the dental pulp, the deciduous tooth, and the periodontium. Several craniofacial structures-such as the mandibular condyle, calvarial bone, cranial suture, and subcutaneous adipose tissue-have been engineered from mesenchymal stem cells, growth factor, and/or gene therapy approaches. As a departure from the reliance of current clinical practice on durable materials such as amalgam, composites, and metallic alloys, biological therapies utilize mesenchymal stem cells, delivered or internally recruited, to generate craniofacial structures in temporary scaffolding biomaterials. Craniofacial tissue engineering is likely to be realized in the foreseeable future, and represents an opportunity that dentistry cannot afford to miss.

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Section: (43) Gene Delivery In Periodontal Tissue Engineeringmentioning

confidence: 99%

Craniofacial Tissue Engineering by Stem Cells

et al. 2006

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“…In the case of periodontal regeneration studies, dental follicle cells, HERS, cementoblasts, and periodontal ligament cells are the focus of research Bosshardt, 2005]. Because of the prominence of signaling molecules in advancing dental tissue differentiation, molecular factors have been applied in vitro and in vivo to manipulate dental tissue differentiation and promote regeneration Thesleff and Tummers, 2001;Anusaksathien et al, 2004;Jin et al, 2004a]. Delivery of cells and molecular factors has been successful with the identifi cation of appropriate scaffolds [MacNeil and Thomas, 1993b;Handa et al, 2002;Young et al, 2002;Jin et al, 2003;Duailibi et al, 2004].…”

Section: Cells Molecular Factors and Scaffoldsmentioning

confidence: 99%

Defining the Roots of Cementum Formation

Popowics

Foster

Swanson

et al. 2005

Cells Tissues Organs

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Significant progress has been seen in research aimed at regeneration of the disease-damaged periodontium. Our own strategy has been to approach periodontal tissue development (i.e. root, cementum, periodontal ligament, and bone) as a source for the identification of key regulators of cellular processes that may be applicable to periodontal tissue repair. Specifically, enamel-like molecules, bone morphogenetic proteins (BMPs), and phosphates have been investigated for their role in altering gene expression and cell functions in follicle cells, periodontal ligament cells, and cementoblasts. Amelogenin, leucine-rich amelogenin peptide, and tyrosine-rich amelogenin peptide have been found to similarly affect cementoblast gene expression and cementoblast-mediated mineralization in vitro; however, these enamel-like factors do not increase cell proliferation as has been observed in cells treated with Emdogain^® (Biora AB, Malmö, Sweden), an enamel matrix derivative. BMP-2 has been found to promote differentiation of follicle cells into a cementoblast/osteoblast phenotype, and BMP-3 is being investigated as a negative regulator of mineralization. The increased ratio of phosphate to pyrophosphate in the local region during root development has been found to significantly enhance the extent of cementum formation in animal models. Furthermore, phosphate has been identified as a regulator of cementoblast SIBLING (small integrin-binding ligand N-linked glycoprotein) gene expression in vitro. These investigations of candidate factors for periodontal regeneration have uncovered mechanisms regulating gene expression and cell function in cells controlling the behavior of periodontal tissues (i.e. follicle cells, periodontal cells, and cementoblasts) and offer new directions to consider for clinical repair of periodontal defects.

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“…proteins and cellular factors by over-expressing desired genes or by blocking their expression, this technique is currently employed in a number of clinical trials where therapeutic genes are introduced in patient tissues. In the field of periodontology, pioneering studies have been conducted using adenovirus to deliver platelet-derived growth factor (PDGF) genes to osteoblasts, cementoblasts, PDL cells and gingival fibroblasts [3][4][5][6][7] demonstrating robust expression of the transgene in targeted tissues and stimulation of tissue regeneration in large periodontal defects. 8 In other studies, adenovirus vectors were used to deliver bone morphogenetic protein (BMP) genes, resulting in enhanced alveolar bone repair, stimulated cementogenesis and PDL fiber formation in rats.…”

Section: Introductionmentioning

confidence: 99%