Cellulose thin-layer chromatographi separation of Rubus fruit anthocyanins

Barritt, B. H.; Torre, L. C.

doi:10.1016/s0021-9673(01)83429-4

Cited by 35 publications

(22 citation statements)

References 6 publications

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“…As can be observed in raspberry, cyanidin and pelargonidin bonded with sugars were detected. By comparing our data with those previously published, eight anthocyanins were identified 15, 33, 42–44. Some differences in anthocyanin fractions of raspberry are evident in the literature.…”

Section: Resultssupporting

confidence: 65%

Analysis of anthocyanins in commercial fruit juices by using nano‐liquid chromatography‐electrospray‐mass spectrometry and high‐performance liquid chromatography with UV‐vis detector

Fanali

Dugo

D’Orazio

et al. 2011

J of Separation Science

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Nano-LC and conventional HPLC techniques were applied for the analysis of anthocyanins present in commercial fruit juices using a capillary column of 100 μm id and a 2.1 mm id narrow-bore C(18) column. Analytes were detected by UV-Vis at 518 nm and ESI-ion trap MS with HPLC and nano-LC, respectively. Commercial blueberry juice (14 anthocyanins detected) was used to optimize chromatographic separation of analytes and other analysis parameters. Qualitative identification of anthocyanins was performed by comparing the recorded mass spectral data with those of published papers. The use of the same mobile phase composition in both techniques revealed that the miniaturized method exhibited shorter analysis time and higher sensitivity than narrow-bore chromatography. Good intra-day and day-to-day precision of retention time was obtained in both methods with values of RSD less than 3.4 and 0.8% for nano-LC and HPLC, respectively. Quantitative analysis was performed by external standard curve calibration of cyanidin-3-O-glucoside standard. Calibration curves were linear in the concentration ranges studied, 0.1-50 and 6-50 μg/mL for HPLC-UV/Vis and nano-LC-MS, respectively. LOD and LOQ values were good for both methods. In addition to commercial blueberry juice, qualitative and quantitative analysis of other juices (e.g. raspberry, sweet cherry and pomegranate) was performed. The optimized nano-LC-MS method allowed an easy and selective identification and quantification of anthocyanins in commercial fruit juices; it offered good results, shorter analysis time and reduced mobile phase volume with respect to narrow-bore HPLC.

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Section: Resultssupporting

confidence: 65%

Analysis of anthocyanins in commercial fruit juices by using nano‐liquid chromatography‐electrospray‐mass spectrometry and high‐performance liquid chromatography with UV‐vis detector

Fanali

Dugo

D’Orazio

et al. 2011

J of Separation Science

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“…Although the four major anthocyanins found in black raspberry have been known for many years (Barritt and Torre, 1973;Harborne and Hall, 1964;Nybom, 1968;Torre and Barritt, 1977), recent studies using high-performance liquid chromatography (HPLC) and mass spectrometry (MS) have identified a wider range of pigments in the fruit than was previously known. Although these studies did not identify the genotypes that their samples came from, small amounts of three anthocyanins, pelargonidin-3-rutinoside, pelargonidin-3-glucoside, and peonidin-3-rutinoside, which had not been previously identified in black raspberry, were detected (Tian et al, 2006;Wu and Prior, 2005).…”

mentioning

confidence: 99%

Inheritance of Phenological, Vegetative, and Fruit Chemistry Traits in Black Raspberry

Dossett¹,

Lee²,

Finn³

2008

J. Amer. Soc. Hort. Sci.

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In recent years, there has been renewed interest in black raspberry (Rubus occidentalis L.) breeding. This has been spurred by an increase in black raspberry consumption resulting from studies that have shown them to be particularly high in anthocyanin content indicating high levels of antioxidants. Present cultivars are ill-adapted to the biotic and abiotic stresses of the Pacific northwestern United States, where the commercial black raspberry industry is centered, and fields must be replanted after three to five seasons. An incomplete partial diallel, consisting of 10 parents and 26 sibling families, was constructed for the study of variation and inheritance of phenological, vegetative, and fruit chemistry traits in black raspberry. Sibling families were established at the Oregon State University Lewis Brown Farm in Corvallis and were arranged as a randomized complete block design with four blocks of one to eight plants. Phenological development and vegetative measurements were recorded for each plant in 2005 and 2006. In addition, 25-berry samples of ripe fruit were collected from each plant. To study variation in fruit chemistry properties, including pH, titratable acidity, percent soluble solids, anthocyanin profiles, and total anthocyanins, additional samples of 25 ripe berries were collected from each plant and pooled by family within blocks. Although there were many striking similarities, strong trends in phenotype based on pedigree were observed for most traits indicating a strong genetic component. General combining ability (GCA) effects were significant and larger than specific combining ability effects for all traits, except for fruit size (mass). With the exception of fruit size, narrow-sense heritability estimates were generally moderate to high (0.30 to 0.91), indicating the potential for breeding progress within the population of plants studied. Despite these results, statistically significant and large GCA values were limited to just a few of the parents, indicating a lack of heritable genetic variation in much of the germplasm base and a need for greater diversity.

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“…These same four anthocyanins have previously been identified in boysenberry fruit by Torre and Barritt using thin‐layer chromatography 15, 16. Previous work with a related Rubus species, red raspberry, has also identified the same four anthocyanins using a combination of paper chromatography and chemical analysis 17.…”

Section: Resultsmentioning

confidence: 62%

LC‐MS identification of anthocyanins in boysenberry extract and anthocyanin metabolites in human urine following dosing

2004

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The anthocyanin composition of boysenberry (Rubus loganbaccus × baileyanus Britt) extract was determined by LC-ESI-MS. Four anthocyanins were identified, all comprising a cyanidinanthocyanidin-type skeleton. The two major components were identified as the disaccharide cyanidin-3-O-sophoroside and the monosaccharide cyanidin-3-O-glucoside. The two less abundant components were identified as the rutinosides, cyanidin-3-O-2 G -glucosylrutinoside and cyanidin-3-O-rutinoside, respectively. These same four anthocyanins were also detected in human urine following a dosing study with boysenberry extract indicating that glycosylated anthocyanins can be absorbed from the gut and excreted intact in the urine. Several anthocyanin metabolites were also detected in the urine and were identified by LC-ESI-MS as monoglucuronides of peonidin, cyanidin and pelargonidin. The results suggest that anthocyanins consumed as part of a diet are bioavailable and are present as intact or metabolized forms in the body.

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Cellulose thin-layer chromatographi separation of Rubus fruit anthocyanins

Cited by 35 publications

References 6 publications

Analysis of anthocyanins in commercial fruit juices by using nano‐liquid chromatography‐electrospray‐mass spectrometry and high‐performance liquid chromatography with UV‐vis detector

Analysis of anthocyanins in commercial fruit juices by using nano‐liquid chromatography‐electrospray‐mass spectrometry and high‐performance liquid chromatography with UV‐vis detector

Inheritance of Phenological, Vegetative, and Fruit Chemistry Traits in Black Raspberry

LC‐MS identification of anthocyanins in boysenberry extract and anthocyanin metabolites in human urine following dosing

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