Cellulose Microfiber Encapsulated Probiotic: Viability, Acid and Bile Tolerance during Storage at Different Temperature

Abstract

This work aimed to analyze the physicochemical properties of cellulose from OPT used in the fabrication of CMF and evaluate the efficacy of the hydrogel CMF as an encapsulant for L. fermentum InaCC B1295 stored at room temperature and in the refrigerator. The Kjeldahl method was used to evaluate the protein content; the gravimetric method was used to determine OPT's ash, moisture, and fiber contents; the Soxhlet method was used to determine the fat content carbohydrates were computed using the difference method. The levels of holocellulose, lignin, and cellulose were also determined. Viability, acid and bile resistance of strain B1295 were evaluated at various temperatures for 35 days. The most abundant component of OPT fiber was cellulose, followed by hemicellulose and lignin. XRD examination revealed that OPT cellulose has a crystal index of 83.40%. FTIR analysis was used to detect the stretching vibrations of the –OH group on cellulose at 3419.03 cm-1. CMF hydrogel from OPT sustained L. fermentum InaCC B1295 survival for up to 28 days at room and refrigerated temperatures. At acidic conditions and in the presence of bile, the viability of L. fermentum InaCC B1295 was excellent, with a drop in cell population of less than 0.2 log CFU/g over 35 days at room and refrigerated temperatures. CMF obtained from OPT can be used as an encapsulant to maintain viability, acid resistance and bile of probiotics. There is still a need for research into the usage of CMF from OPT in combination with other encapsulants to extend the storage life of L. fermentum InaCC B1295. Doi: 10.28991/ESJ-2022-06-01-08 Full Text: PDF

Physicochemical characteristics of oil palm frond and application of CMF Hydrogel as a natural encapsulant for probiotic

“…Viability, acid, and bile tolerance of probiotics. Probiotic encapsulated by CMFH from OPF was evaluated for viability, acid, and bile [18][19][20][21].…”

Potensi Probiotik Bakteri Asam Laktat Asal Madu dari Tiga Jenis Lebah yang Berbeda

“…BAL asal kefir memiliki ketahanan yang lebih lemah dari BAL isolat asal madu, dengan penurunan sebesar 0,69-1,59 log CFU/mL (Yusuf et al, 2020). Mikroenkapsulasi dengan Cellulose microfiber dapat meningkatkan ketahanan terhadap garam empedu (Pato et al, 2022).…”

“…Dibandingkan dengan isolat BAL asal kefir yang mengalami penurunan sebesar 0,57 and 2,04 log CFU/mL , ketahanan BAL asal madu lebih tahan pH rendah. Ketahanan BAL terhadap pH dapat diperbaiki dengan enkapsulasi dalam cellulose microfiber (Pato et al, 2022), namun BAL asal madu tanpa enkapsulasi telah memiliki ketahanan terhadap asam yang sangat baik.…”

“…Dengan penurunan sebesar 0,5 log CFU/mL, jumlah BAL yang mencapai kolon di atas 8 log CFU. Jumlah di atas 7,0 log CFU yang dapat mencapai kolon diperlukan untuk memberikan manfaat terapi (Pato et al, 2022). Kedua belas isolat ini berpotensi sebagai probiotik.…”

Estudo da formação de micropartículas fitoterápicas contendo extrato de folhas de >i<Cynara cardunculus>/i< L. por tecnologia supercrítica e por gelificação iônica para emprego no desenvolvimento de >i<sorbet>/i< funcional

“…Dessa forma, alguns estudos envolvendo microencapsulação/impregnação estão sendo desenvolvidos para garantir que as células microbianas cheguem viáveis no intestino para que possam colonizá-lo e assim exercer os possíveis efeitos benéficos à saúde após seu consumo. A microencapsulação também apresenta a vantagem de permitir a liberação controlada do probiótico no intestino grosso que é o local-alvo de sua ação (Varela-Pérez et al, 2022;Kowalska et al, 2022;Pato et al, 2022). Além dos benefícios apresentados microencapsulação é uma solução promissora para os problemas relacionados à alterações organolépticas durante o armazenamento dos alimentos funcionais devido à bioatividade das células probióticas vivas (Liao et al, 2017).…”