Cellulose-based polymeric liquid crystals as a biomimetic modifier for suppressing protein adsorption

Situ, Fangmin; Tan, Ruizhe; Gong, Lei; Zha, Zhengang; Tu, Mei; Zeng, Rong; Wu, Hao; Zhang, Jiaqing; Zheng, Li

doi:10.1007/s11595-015-1163-0

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Recently, thin layer materials with wicking power, such as paper, sponge rubber and glass fiber paper, have been employed to achieve bioseparation as a function of the analytes' physical and chemical properties. [20][21][22][23][24][25][26][27] The primary advances in the development and use of LFAs has focused on strategies to affect greater selectivity and sensitivity, 14,28 e.g. focusing on the detection event.…”

Section: Introductionmentioning

confidence: 99%

Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films

Zhang

Jiang

Willett

et al. 2016

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Presented here is a novel implementation of polypropylene capillary-channeled polymer (C-CP) films, functionalized for bioaffinity separations and implemented as a platform for lateral flow (immuno) assays. The parallel ∼80 μm × 80 μm channels pass test solutions down the 30 mm film length via spontaneous wicking action, setting up the possibility for immobilizing different capture agents in the respective channels. The base-film modification process is divided into two steps: ultraviolet light treatment to improve hydrophillicity of the polypropylene substrate and the physical adsorption of a functionalized lipid tethered ligand (LTL) as a selective capture agent. The entire modification procedure is performed under ambient conditions in an aqueous solution without extreme pH conditions. In this demonstration, physical adsorption of a biotinylated-LTL onto the UV-treated PP surface selectively captures Texas Red-labeled streptavidin (SAv-TR) in the presence of enhanced green fluorescence protein (EGFP), which passes without retention in less than 5 s. In addition to the fluorescence imaging of the protein solutes, matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) was used to confirm the formation of the LTL-SAv conjugates on the channel surface as well as to demonstrate an alternative means of probing the capture step. The present effort sets the groundwork for further development of C-CP films as a parallel, multi-analyte LFA platform; a format that to-date has not been described.

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Section: Introductionmentioning

confidence: 99%

Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films

Zhang

Jiang

Willett

et al. 2016

Analyst

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Determination of Bovine Serum Albumin Adsorption Capacity of Newly Obtained Cellulose extracted from Glycyrrhiza glabra (Licorice)

2016

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Exploration of biodegradable and renewable composites ingredients is of great importance. Up to now, roots extract of Glycyrrhiza glabra has been used for different medicinal purposes but remaining cellulose-rich residues have not been evaluated for isolation of any green materials like cellulose. Here in this study, cellulose was successfully isolated from the roots of G. glabra by using conventional cellulose extraction method. The obtained product was characterized using FT-IR, XRD, and SEM. FT-IR and XRD analyses confirmed the identity of the product. The crystallinity index of cellulose isolates was calculated from XRD data as 70%. The cellulose content of the roots on dry basis was recorded as 48.5%. Three types of surface morphologies were observed: smooth non-porous surface, surface with fibril bundles, and porous surface having thin cellulose fibrils. In addition, bovine serum albumin (BSA) adsorption capacity of G. glabra cellulose was determined and compared with that of commercial cellulose. Newly obtained cellulose from the roots of G. glabra exhibited a higher affinity for BSA protein at neutral pH.

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Functional surface coatings from tailor-made long-chain hydroxypropyl cellulose ester nanoparticles

2018

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Cellulose-based polymeric liquid crystals as a biomimetic modifier for suppressing protein adsorption

Cited by 3 publications

References 21 publications

Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films

Parallel, open-channel lateral flow (immuno) assay substrate based on capillary-channeled polymer films

Determination of Bovine Serum Albumin Adsorption Capacity of Newly Obtained Cellulose extracted from Glycyrrhiza glabra (Licorice)

Functional surface coatings from tailor-made long-chain hydroxypropyl cellulose ester nanoparticles

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