Cellulose as an efficient matrix for lipase and transaminase immobilization

Souza, Stefânia P. de; Itabaiana, Ivaldo; Silva, Guilherme M. A.; Miranda, Leandro S. M.; Santiago, Marcelo F.; Lam, Frank Leung Yuk; Dawood, Ayad W. H.; Bornscheuer, Uwe T.; Souza, Rodrigo O. M. A. de

doi:10.1039/c5ra24976g

Cited by 40 publications

(34 citation statements)

References 31 publications

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“…For such processes, efficient immobilization of the enzyme becomes significant. Several reports dealt with the successful immobilization of ATAs using different supports (e.g., chitosan, functionalized cellulose, polystyrene or methacrylate beads as well as concepts for the co‐immobilization of the cofactor PLP . For our example here, the immobilization via metal affinity resins was choosen because this is a convenient method for the immobilization of recombinant proteins without pre‐treatment or activating of supports with for example, cross‐linking agents.…”

Section: Methodsmentioning

confidence: 99%

Combination of the Suzuki–Miyaura Cross‐Coupling Reaction with Engineered Transaminases

Dawood

Bassut

Souza

et al. 2018

Chemistry A European J

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The combination of enzymatic and chemical reaction steps is one important area of research in organic synthesis, preferentially as cascade reactions in one-pot to improve total conversion and achieve high operational stability. Here, the combination of the Suzuki-Miyaura reaction is described to synthesize biaryl compounds followed by a transamination reaction. Careful optimization of the reaction conditions required for the chemo- and biocatalysis reaction enabled an efficient two-step-one-pot reaction yielding the final chiral amines with excellent optical purity (>99 % ee) in up to 84 % total conversion. Key to the success was the protein engineering of the amine transaminases from Asperguillus fumigatus (4CHI-TA) where single alanine mutations increased the conversion up to 2.3-fold. Finally, the transfer to a continuous flow system after immobilization of the best 4CHI-TA variant is demonstrated.

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Section: Methodsmentioning

confidence: 99%

Combination of the Suzuki–Miyaura Cross‐Coupling Reaction with Engineered Transaminases

Dawood

Bassut

Souza

et al. 2018

Chemistry A European J

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“…The immobilization by covalent attachment using various support materials such as chitosan is also widely reported [96][97][98][99]. De Souza et al [100] recently utilized cellulose for immobilization of ω-TAVf. The ω-TAVf immobilized in the epoxy-functionalized cellulose conferred excellent temperature stability and reusability, wherein enzyme was consistently active over the broad range of temperature from 30 to 60 • C and for four repetitive cycles in the asymmetric synthesis of (S)-phenylethylamine.…”

Section: Enzyme Immobilizationmentioning

confidence: 99%

Recent Advances in ω-Transaminase-Mediated Biocatalysis for the Enantioselective Synthesis of Chiral Amines

et al. 2018

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Chiral amines are important components of 40-45% of small molecule pharmaceuticals and many other industrially important fine chemicals and agrochemicals. Recent advances in synthetic applications of ω-transaminases for the production of chiral amines are reviewed herein. Although a new pool of potential ω-transaminases is being continuously screened and characterized from various microbial strains, their industrial application is limited by factors such as disfavored reaction equilibrium, poor substrate scope, and product inhibition. We present a closer look at recent developments in overcoming these challenges by various reaction engineering approaches. Furthermore, protein engineering techniques, which play a crucial role in improving the substrate scope of these biocatalysts and their operational stability, are also presented. Last, the incorporation of ω-transaminases in multi-enzymatic cascades, which significantly improves their synthetic applicability in the synthesis of complex chemical compounds, is detailed. This analysis of recent advances shows that ω-transaminases will continue to provide an efficient alternative to conventional catalysis for the synthesis of enantiomerically pure amines.

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“…The well-packed crystalline regions of the cellulose fibrils are tough and fibrous, and this polymer is also characteristically insoluble in water and impervious to most organic solvents. Most importantly, this biopolymer is naturally abundant in hydroxyl groups [24,25] which permit further chemical modifications. Four kinds of cellulose allomorphs exist, viz.…”

Section: Cellulosementioning

confidence: 99%

On the Renewable Polymers from Agro-industrial Biomass: A Mini Review

Wahab

Gowon²,

Elias

2019

J. Idn. Chem. Soc.

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Plant biomass is the most abundant natural resources on earth. However, current strategies for the utilization of agricultural biomass is far from efficient, thus environmental issues related to incompetent management of biomass prevail. Innovative handling of surplus biomass can yield several rewards, which includes renewability and sustainability of the commodity as feedstock for industrial and energy purposes. In fact, an array of different parts of a plant or agro-industrial biomass, for instance, shell, husks, wood, and leaves were successfully converted into advanced carbon materials, for use as absorbent, catalyst enzyme support, electrode, etc. In this review, an extensive literature survey related to areas of renewable sources of biopolymer in both the agricultural and industrial sectors were performed. Information on their industrial value and uses, the fundamentals of their extraction alongside the benefits and major drawbacks of their utilization, are also highlighted. We aim to show that the smart utilization of unwanted agro-industrial biomass encompasses a portion of a bigger scheme that intelligently uses biomass to complement current agricultural advancements that create smart crops and growing them using cleverly designed technology. The best part of this “Waste to Wealth” concept is that every part of the crop is fully utilized. However, a set of clear criteria must be in place to ensure a sustained momentum, so that the green approach of responsible biomass utilization will be fully embraced by nations worldwide.

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Cellulose as an efficient matrix for lipase and transaminase immobilization

Abstract: Continuous-flow biocatalysis using immobilized lipases and transaminases in order to synthesize chiral alcohols and amines respectively.

Cited by 40 publications

References 31 publications

Combination of the Suzuki–Miyaura Cross‐Coupling Reaction with Engineered Transaminases

Combination of the Suzuki–Miyaura Cross‐Coupling Reaction with Engineered Transaminases

Recent Advances in ω-Transaminase-Mediated Biocatalysis for the Enantioselective Synthesis of Chiral Amines

On the Renewable Polymers from Agro-industrial Biomass: A Mini Review

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