Cellules dendritiques des muqueuses et de la peau

Borgne, Marie Le; Dubois, Bertrand; Kaiserlian, Dominique

doi:10.1051/medsci/20072310819

Cited by 10 publications

(7 citation statements)

References 36 publications

(42 reference statements)

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“…pDUV5 was administered intradermally, the skin being an attractive site for vaccination given the abundance of leukocytes compared for example to muscle [28]. EGT leads to the transfection of APCs that migrate to the lymph nodes allowing the priming and differentiating of CD8 T-cells into cytolytic effectors [29]. In healthy dogs, electroporated pNTC-DUV5 induced a cTERT specific cellular immune response after three immunizations.…”

Section: Discussionmentioning

confidence: 99%

A DNA telomerase vaccine for canine cancer immunotherapy

Thalmensi¹,

Pliquet²,

Liard³

et al. 2019

Oncotarget

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Telomerase reverse transcriptase (TERT) is highly expressed in more than 90% of canine cancer cells and low to absent in normal cells. Given that immune tolerance to telomerase is easily broken both naturally and experimentally, telomerase is an attractive tumor associated antigen for cancer immunotherapy. Indeed, therapeutic trials using human telomerase peptides have been performed. We have developed an immunogenic yet catalytically inactive human telomerase DNA construct that is in clinical trials with patients presenting solid tumors. Paralleling this human construct, we have developed a canine telomerase DNA vaccine, called pDUV5. When administered intradermally to mice combined with electrogene transfer, pDUV5 induced canine TERT specific cytotoxic T-cells as measured by IFN-γ ELISpot assay. Intradermal vaccination of healthy dogs with 400 μg of pDUV5 generated strong, broad and long lasting TERT specific cellular immune responses. In vitro immunization with cTERT peptides revealed the maintenance of cTERT specific T-cells in PBMCs from tumor bearing dogs showing that this repertoire was not depleted. This study highlights the potential of pDUV5 as a cancer vaccine and supports its evaluation for the treatment of spontaneous canine tumors.

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Section: Discussionmentioning

confidence: 99%

A DNA telomerase vaccine for canine cancer immunotherapy

Thalmensi¹,

Pliquet²,

Liard³

et al. 2019

Oncotarget

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show abstract

“…Ainsi, dans le sang, la rate, les amygdales et les ganglions lymphatiques, on observe des cellules dendritiques résidentes BDCA1 + (blood dendritic cell antigen 1) et Clec9A + (C-type lectin domain family 9 member A). Dans la peau, on trouve trois populations de cellules dendritiques migratoires : les cellules dendritiques dermiques CD1a + et CD14 + , et les cellules de Langerhans épidermiques [33]. Des cellules dendritiques épidermiques inflammatoires ont été décrites en 1996 dans la peau de patients présentant une dermatite atopique [23].…”

Section: Les Cellules Dendritiques Inflammatoires Humainesunclassified

Les cellules dendritiques inflammatoires

Ségura

Amigorena

2014

Med Sci (Paris)

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“…Il sera donc intéressant d'analyser si une population de DDC langérine + existe chez l'homme et pourrait par exemple constituer, dans le cas de lésions de l'épiderme, une barrière empêchant l'entrée du VIH-1. Ainsi, une meilleure connaissance de cette population devrait permettre de progresser dans la compréhension de certains modèles infectieux et de faire évoluer les approches vaccinales ciblant les antigènes sur la peau [8]. …”

Section: En Conclusionunclassified