Cellulase Supplementation Does Not Improve the Digestibility of a High-Forage Diet in Horses

O′Connor-Robison, C.I.; Nielsen, B.D.; Morris, RJ

doi:10.1016/j.jevs.2007.10.014

Cited by 14 publications

(10 citation statements)

References 6 publications

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“…This may be partly due to the greater nutrients digestibility with enzyme supplementation, which is consistent with previous results [9]. However, O'Connor-Robison et al [6] did not find a feed intake effect with cellulase in horse diets. To our knowledge, there are no in vivo studies available on cecal fermentation in horses.…”

Section: Feed Intake and Nutrient Digestibilitysupporting

confidence: 87%

“…In contrast, Murray et al [9] reported a significant reduction in in vivo digestibility of the fibrous fractions of enzyme-treated high-temperature dried lucerne and ensiled lucerne. Similarly, O'Connor-Robison et al [6] fed Arabian geldings a hay-based diet supplemented with cellulase and noted that cellulase addition decreased digestion of the fiber components.…”

Section: Introductionmentioning

confidence: 99%

“…The large intestine of the horse is a fermentation system similar to the rumen [5]. Microorganisms living in the rumen of ruminant animals and in the cecum of horses give them the ability to breakdown fibers by microbial fermentation to meet energy demands [6].…”

Section: Introductionmentioning

confidence: 99%

“…Supplementing the diet of horses with exogenous fibrolytic enzymes has gained substantial interest in recent years [6,8]. However, the potential of exogenous enzymes to enhance the digestion of plant structural carbohydrates in the hindgut of the equine working in synergism with endogenous microorganisms is inconclusive [8].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Salem

Elghandour

Kholif

et al. 2015

Journal of Equine Veterinary Science

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a b s t r a c tSixteen Quarter Horse mares (450 to 500-kg body weight) were used in a complete randomized design to determine the effects of feeding a high fiber diet with or without exogenous fibrolytic enzymes on nutrient digestion, blood chemistry, fecal coliform count, and in vitro fecal fermentation. The treatments comprised feeding the horses (1) a basal diet without enzyme addition (control); (2) control diet plus cellulase at 10 mL/mare/ d (CELL); (3) control diet plus xylanase at 10 mL/mare/d (XYL); or (4) control diet plus a mixture of 5 mL cellulase and 5 mL xylanase/mare/d (CX). The basal concentrate diet consisted of a mixture of 50% commercial concentrate and 50% wheat bran fed at 4 kg/ horse, offered twice daily at 04:00 and 16:00 hours, and oat straw offered ad libitum at 05:00 and 17:00 hours. The enzyme allocation for each day was mixed with 1 kg of concentrate diet at 04:00 hours, and the experiment lasted for 15 days comprising 10 days of adaptation and 5 days for sample collection. The in vitro cecal fermentation with addition of 2 mL/g dry matter (DM) of each enzyme (CELL, XYL, and CX) to a basal diet of oat straw and concentrates mixture (1:1 DM) as a substrate was carried out. The mares fed enzyme-supplemented diets had greater (P < .01) oat straw and total nutrients intakes compared with the control diet. Feeding enzyme-supplemented diets increased total nutrients digestibility (P < .05) and blood total protein (P ¼ .0277) compared with the control. Feeding XYL-supplemented diet increased blood alanine transaminase and aspartate aminotransferase concentrations (P < .05) compared with control treatment. Lower fecal coliform count was obtained (P ¼ .0114) with mares fed CX diet compared with control mares. The XYL and CX treatments had decreased asymptotic gas production (GP) (P ¼ .0173) with lower rate of GP (P ¼ .0412) compared with CELL treatment. CELL and XYL treatments had decreased (P ¼ .0394) lag times compared with control and CX treatments. At 24 hours of incubation, CELL and XYL treatments decreased methane production (P ¼ .0131), whereas CX treatment increased its production at 48 hours (P ¼ .0202) compared with control treatment. No effect was observed (P > .05) with enzymes addition on carbon dioxide production at different hours of incubation compared with control treatment. Higher in vitro DM degradability values (P ¼ .0092) were obtained with the enzyme

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Section: Feed Intake and Nutrient Digestibilitysupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Salem

Elghandour

Kholif

et al. 2015

Journal of Equine Veterinary Science

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“…Cellulases belong to a large family of glycoside hydrolases and are common to a variety of bacteria, fungi, plants, and animals. Cellulases and related enzymes are widely used today in agriculture and research [Graminha et al, 2008;Lee et al, 2008;O'Connor-Robison et al, 2007;Zhao et al, 2010].…”

Section: Introductionmentioning

confidence: 99%

Electrotransformation and Expression of Cellulase Genes in Wild-Type <b><i>Lactobacillus reuteri</i></b>

Yang

Zhang

et al. 2012

Microb Physiol

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Two cellulase genes, Cel15 and Cel73, were amplified from Bacillus subtilis genome DNA in a previous study. Two integrative vectors, pLEM4153 and pLEM4154, containing the genes Cel15 and Cel73, respectively, were constructed and successfully electroporated into the wild-type Lactobacillus reuteri which was isolated from chick guts through an optimized procedure. Two recombinant L. reuteri were selected from a Man, Rogosa, and Sharp (MRS) plate with 10 µg/ml erythromycin, and named L. reuteri XNY-Cel15 and L. reuteri XNY-Cel73, respectively. To verify the transcription and expression of the two cellulase genes in the recombinant L. reuteri strains, the mRNA relative quantity (RQ) and the cellulase activity were determined. The mRNA RQ of Cel15 in L. reuteri XNY-Cel15 is 1,8849.5, and that of Cel73 in L. reuteri XNY-Cel73 is 1,388, and the cellulase activity of the modified MRS broth cultured with L. reuteri XNY-Cel15 was 0.158 U/ml, whereas that with L. reuteri XNY-Cel73 was 0.15 U/ml.

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What the hay: Predicting equine voluntary forage intake using a meta-analysis approach

Leishman,

Sahar,

Cieslar

et al. 2024

animal

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Cellulase Supplementation Does Not Improve the Digestibility of a High-Forage Diet in Horses

Cited by 14 publications

References 6 publications

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

The Effect of Feeding Horses a High Fiber Diet With or Without Exogenous Fibrolytic Enzymes Supplementation on Nutrient Digestion, Blood Chemistry, Fecal Coliform Count, and In Vitro Fecal Fermentation

Electrotransformation and Expression of Cellulase Genes in Wild-Type <b><i>Lactobacillus reuteri</i></b>

What the hay: Predicting equine voluntary forage intake using a meta-analysis approach

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