Cellulase Enzyme Production From Rice Straw Using Solid State Fermentation and Fungi Aspergillus niger ITBCC L74

Maftukhah, Siti; Abdullah, Abdullah

doi:10.1051/matecconf/201815601010

Cited by 14 publications

(8 citation statements)

References 30 publications

(22 reference statements)

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“…The peak enzyme activity (121.036 U/mL) was recorded (Figure 2A) specifically on day 4 of fermentation. This situation suggested a feasible harvest (enzyme) at about 96 h (on day 4) of fermentation, similar to the findings published elsewhere (Abubakar and Oloyede, 2013;Costa et al, 2021;Maftukhah and Abdullah, 2018;Okoye et al, 2013). The incubation period, however, would depend on a number of factors, such as the availability/level of nutrients, fermentation environment, nature of fermenting organisms and the physiological conditions influencing the (fermentation) process (Patil and Dayanand, 2006;Shivasharanappa et al, 2014).…”

Section: Enzyme Production Ammonium Sulfate Precipitation Profile And...supporting

confidence: 89%

An enhanced fat extraction from Pycnanthus angolensis (African nutmeg) seeds using cellulase from Aspergillus niger strain BC23

Chiwetalu

Ossai

Enechi

et al. 2022

Qual. Assur. Saf. Crops Foods

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A promising extraction method for obtaining fat from Pycnanthus angolensis seeds through Aspergillus niger strain BC23 enzyme pretreatment and particle size reduction is presented in this work. The employed A. niger enzyme was produced from corn bagasse waste material, which served as the only source of carbon. P. angolensis seeds were dried, pulverized and subsequently sieved into two different particle sizes of 1.0 mm and 1.4 mm. This was followed by enzyme pretreatment prior to fat extraction. Results demonstrated that while the peak enzyme activity (121.036 U/mL) occurred on day 4 of fermentation, the maximum cellulase activity could be achieved at 60% saturation of ammonium sulfate purification. Compared with the results of water-treated and untreated seed samples, the fat yield, free fatty acid and iodine values of the enzyme-treated seed samples were considerably higher (P < 0.05). As peroxide value was less in the enzyme-assisted extracted fat, which pointed to a good-quality oil, the decreased refractive index suggested peak unsaturation. Despite considerable differences (P < 0.05) in refractive indices, fat’s specific gravity matched (P > 0.05) when enzyme-treated, water-treated and untreated samples were compared. On the whole, size reduction of P. angolensis seeds pretreated with A. niger cellulase enzyme to improve both fat quality and yield implied it as a useful inventory for confectionary as well as cosmetic industries.

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Section: Enzyme Production Ammonium Sulfate Precipitation Profile And...supporting

confidence: 89%

An enhanced fat extraction from Pycnanthus angolensis (African nutmeg) seeds using cellulase from Aspergillus niger strain BC23

Chiwetalu

Ossai

Enechi

et al. 2022

Qual. Assur. Saf. Crops Foods

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“…The enzyme assay was carried out on each T. reesei and A. awamori sample for the three incubation times at 3, 5, and 7 days post-incorporation (DPI) using the method of Maftukhah and Abdullah [ 16 ]. In brief, a rolled Whatman no.1 filter paper strip, of the dimensions 1.0 × 6 cm (50 mg), was placed into each assay tube.…”

Section: Methodsmentioning

confidence: 99%

Cellulase Enzyme Production from Filamentous Fungi Trichoderma reesei and Aspergillus awamori in Submerged Fermentation with Rice Straw

Naher

Fatin

Sheikh

et al. 2021

JoF

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Fungi are a diverse group of microorganisms that play many roles in human livelihoods. However, the isolation of potential fungal species is the key factor to their utilization in different sectors, including the enzyme industry. Hence, in this study, we used two different fungal repositories—soil and weed leaves—to isolate filamentous fungi and evaluate their potential to produce the cellulase enzyme. The fungal strains were isolated using dichloran rose bengal agar (DRBA) and potato dextrose agar (PDA). For cellulase enzyme production, a rice straw submerged fermentation process was used. The enzyme production was carried out at the different incubation times of 3, 5, and 7 days of culture in submerged conditions with rice straw. Fungal identification studies by morphological and molecular methods showed that the soil colonies matched with Trichoderma reesei, and the weed leaf colonies matched with Aspergillus awamori. These species were coded as T. reesei UMK04 and A. awamori UMK02, respectively. This is the first report of A. awamori UMK02 isolation in Malaysian agriculture. The results of cellulase production using the two fungi incorporated with rice straw submerged fermentation showed that T. reesei produced a higher amount of cellulase at Day 5 (27.04 U/mg of dry weight) as compared with A. awamori (15.19 U/mg of dry weight), and the concentration was significantly different (p < 0.05). Our results imply that T. reesei can be utilized for cellulase production using rice straw.

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“…Evelyn [21] reported that Eupenicillium javanicum can produce cellulase using pineapple crown leaves waste with the highest activity after 4 days of incubation at 35 °C through solid-state fermentation. Maftukhah and Abdullah [22] also reported that A. niger can produce cellulase from rice straw under the SSF method with the optimum incubation time being 6 days at 30 °C. Trichoderma viride can produce cellulase using maize husk silage with the highest activity after 7 days of incubation at 30 °C through the SSF method [23].…”

Section: Cellulase Productionmentioning

confidence: 99%

Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate

Trianawati¹,

Winarsa²,

Siswoyo³

et al. 2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Cellulase can be produced by a microorganism, such as fungi. The cellulolytic microorganism used in this study was Paecilomyces lilacinus ICP1. P. lilacinus ICP1 is one of the indigenous fungi from coffee pulp that has been known to produce cellulose. Coffee pulp is expected to be able to replace the use of pure cellulose substrate because of its high cellulose content of 63% and other organic materials that can support the growth of cellulolytic microorganisms. One method that is often used for enzyme production by utilizing agricultural or plantation waste is Solid State Fermentation (SSF). Cellulase production by Paecilomyces lilacinus ICP1 using coffee pulp waste as a substrate has been investigated. Through Solid State Fermentation of coffee pulp substrate based the P. lilacinus ICP1 can produce cellulase up to 0.17 U/ml with 3 days of incubation at 30 °C. Cellulase activity was measured base on reducing sugar produced using the Somogyi-Nelson method against 0.5% carboxymethyl cellulose in 20 mM acetate buffer pH 5. The crude cellulase was then dialyzed through a cellulose membrane with 12-14 kDa pores in the same buffer. In this step, the activity was 0.47 U/ml, an increase about three times from the initial. These results proved that P. lilacinus ICP1 could secrete extracellular cellulase through Solid State Fermentation using coffee pulp waste without any nutrients added so that it may become a strategy industrial scale to produce cellulase cheaply.

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Cellulase Enzyme Production From Rice Straw Using Solid State Fermentation and Fungi Aspergillus niger ITBCC L74

Cited by 14 publications

References 30 publications

An enhanced fat extraction from Pycnanthus angolensis (African nutmeg) seeds using cellulase from Aspergillus niger strain BC23

An enhanced fat extraction from Pycnanthus angolensis (African nutmeg) seeds using cellulase from Aspergillus niger strain BC23

Cellulase Enzyme Production from Filamentous Fungi Trichoderma reesei and Aspergillus awamori in Submerged Fermentation with Rice Straw

Cellulase Production from Paecilomyces Lilacinus ICP1 Using Coffee Pulp as Substrate

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