Cellular volume regulation in salinity stressed molluscs: The response ofNoetia ponderosa (Arcidae) red blood cells to osmotic variation

Amende, Lynn M.; Pierce, Sidney K.

doi:10.1007/bf00691562

Cited by 43 publications

(10 citation statements)

References 35 publications

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“…46 This is probably because of large change in organic compound content occurs over a period of days in mollusc tissues. [47][48][49] On the contrary, fish and other vertebrates have the ability to use organic osmolytes also in response to an acute hypotonic shock. 43,44 In conclusion, it seems evident that the digestive cells of M. galloprovincialis possess regulatory mechanisms, involving inorganic ions such as K + and Cl À , allowing them to cope with an acute hypotonic stress performing a partial RVD and thus avoid a large increase of cell volume in order to maintain a proper function.…”

Section: Discussionmentioning

confidence: 99%

“…The use of organic osmolytes to cope with the stress of chronic exposure to dilute seawater has been already observed in other molluscs . This is probably because of large change in organic compound content occurs over a period of days in mollusc tissues . On the contrary, fish and other vertebrates have the ability to use organic osmolytes also in response to an acute hypotonic shock …”

Section: Discussionmentioning

confidence: 99%

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Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Torre

Trischitta

Corsaro

et al. 2012

Cell Biochemistry & Function

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The response of isolated digestive cells of the digestive gland of Mytilus galloprovincialis to hypotonic shock was studied using videometric methods. The isolated cells exposed to a rapid change (from 1100 to 800 mosmol kg(-1) ) of the bathing solution osmolality swelled but thereafter underwent a regulatory volume decrease (RVD), tending to recover the original size. When the hypotonic stress was applied in the presence of quinine and glibenclamide, known inhibitors of swelling activated ion channels, the cells did not exhibit an RVD response; in addition, they showed a larger increase in size in respect to control cells. These observations suggest that the digestive cells of the digestive gland have the machinery to cope with the hyposmotic shock allowing them to exhibit a small but significant RVD preventing an excessive increase in cell size. The pharmacological treatment of digestive cells during the RVD experiments suggests that cell volume is regulated by K(+) and Cl(-) efflux followed by an obliged water efflux from the cell. The involvement of organic osmolytes such as taurine and betaine seems to be excluded by NMR measurement on digestive cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Torre

Trischitta

Corsaro

et al. 2012

Cell Biochemistry & Function

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show abstract

“…A cell’s osmolarity is the sum of the concentrations of the various ion species and many proteins and other organic compounds inside the cell. The main ions include Na + , K + , Ca 2+ , and Cl - , and bioorganic molecules include free amino acids ( Siebers et al, 1972 ; Amende and Pierce, 1980 ). Na + and Cl - are the principal osmotically active solutes in the hemolymph.…”

Section: Introductionmentioning

confidence: 99%

Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai

Jia

Liu

2018

Front. Physiol.

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Na+/K+-ATPase (NKA) belongs to the P-type ATPase family, whose members are located in the cell membrane and are distributed in diverse tissues and cells. The main function of the NKA is to regulate osmotic pressure. To better understand the role of NKA in osmoregulation, we first cloned and characterized the full-length cDNAs of NKA α subunit and β subunit from Pacific abalone Haliotis discus hannai in the current study. The predicted protein sequence of the NKA α subunit, as the catalytic subunit, was well conserved. In contrast, the protein sequence of the β subunit had low similarity with those of other species. Phylogenetic analysis revealed that both the α and β subunits of the NKA protein of Pacific abalone were clustered with those of the Gastropoda. Then, the relationship between salinity changes and the NKA was investigated. Sudden salinity changes (with low-salinity seawater (LSW) or high-salinity seawater (HSW)) led to clear changes in ion concentration (Na+ and K+) in hemolymph; however, the relative stability of ion concentrations in tissue revealed that Pacific abalone has a strong osmotic pressure regulation ability when faced with these salinity changes. Meanwhile, the expression and activity of the NKA was significantly decreased (in LSW group) or increased (in HSW group) during the ion concentration re-establishing stages, which was consistent with the coordinated regulation of ion concentration in hemolymph. Moreover, a positive correlation between cyclic adenosine monophosphate (cAMP) concentrations and NKA mRNA expression (NKA activity) was observed in mantle and gill. Therefore, the sudden salinity changes may affect NKA transcription activation, translation and enzyme activity via a cAMP-mediated pathway.

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“…Further, suboptimal low salinity causes added stress to the abalone Haliotis varia Linnaeus when exposed to toxic substances (Lasut 1999). As in other marine mollusks, cell volume is regulated during variation in external salinity using intracellular free amino acids as osmotic solutes (Baginski and Pierce 1975;Amende and Pierce 1980) causing changes in water content and weight of abalone (Morash and Alter 2016). In our study, the lowest salinity (20 ppt) is the least favorable to the growth of H. diversicolor.…”

Section: Discussionmentioning

confidence: 77%

Effects of salinity on the growth and mucous cells of the abalone Haliotis diversicolor Reeve, 1846

Creencia

Noro

2018

Int Aquat Res

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This study was conducted to determine the influence of salinity on the growth of abalone Haliotis diversicolor Reeve, including the density and size of mucous cells. Abalone individuals were reared in the laboratory at salinities of 20, 25, 31, 35 and 40 ppt. The mucous cells of the lips, gills and digestive gut of H. diversicolor, which react to some forms of stress such as suboptimal salinity, were characterized following staining with Alcian Blue-Periodic Acid-Schiff's Reagent (AB-PAS). The specific growth rate in wet weight and shell length of H. diversicolor were highest at 31 ppt and lowest at 20 ppt (0.52 vs 0.15% d -1 , and 0.058 vs 0.021 mm d -1 , respectively). The abalone H. diversicolor tolerated salinity fluctuations within the range of 20-40 ppt, but growth was optimum at 25-35 ppt. Mucous cells of the lips and gills showed significant differences (ANOVA, df = 4, P = \0.001) in cell density and cell size, being less dense and larger at 31 ppt than at 40 ppt, which could be an effect of osmotic and ionic regulation. Consistent with reports in literature, salinity ranges of 25-35 ppt are suitable for growth of H. diversicolor. Results of this study indicated that areas with such salinity are favorable for stock enhancement and mariculture of the abalone H. diversicolor.

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Cellular volume regulation in salinity stressed molluscs: The response ofNoetia ponderosa (Arcidae) red blood cells to osmotic variation

Cited by 43 publications

References 35 publications

Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Digestive cells from Mytilus galloprovincialis show a partial regulatory volume decrease following acute hypotonic stress through mechanisms involving inorganic ions

Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai

Effects of salinity on the growth and mucous cells of the abalone Haliotis diversicolor Reeve, 1846

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