Cellular uptake and phototoxicity of surface-modified fluorescent nanodiamonds

Weng, Mao Feng; Chang, Bo-Jui; Chiang, Su‐Yu; Wang, Niann‐Shiah; Niu, Huan

doi:10.1016/j.diamond.2011.12.035

Cited by 29 publications

(38 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, Weng et al (2012) observed an approximately two-fold reduction in the proliferation rate of HeLa cells treated with 10 lg/ml HPHT NDs modified with amino groups or transferrin. Marcon et al (2010) obtained analogous results with DNDs.…”

Section: In Vitro Biocompatibility Of Hpht Ndsmentioning

confidence: 90%

“…Notably, the ND always bears a mixture of various moieties because the reactions never reach 100 % completion grafting, on the other hand, creates a stronger, much more specific connection between a molecule and the ND surface (Huang and Chang 2004). These connections are usually realized via amide bonds; either an amino group of a biomolecule reacts with a carboxyl group on the ND surface (Dahoumane et al 2009;Sreenivasan et al 2011;Fu et al 2007Fu et al , 2012Cheng et al 2007;Wei et al 2010;Zhang et al 2009a;Mkandawire et al 2009;Mohan et al 2010;Li and Zhou 2010;Weng et al 2009Weng et al , 2012 or vice versa (Hens et al 2008). Silane linkers can be also used to connect biomolecules to the ND surface by forming a covalent amide bond (Krüger et al 2006;Krueger et al 2008) or by the reaction of isocyanate with an amino group (Martín et al 2010a).…”

Section: Biomolecules On the Nd Surfacementioning

confidence: 98%

“…The rate of cellular uptake in the presence or absence of serum is, however, determined by many factors. Weng et al (2012) showed that the interaction of HPHT ND-COOH with cells is negligible. In serumsupplemented medium, the negatively charged ND surface is repelled by the negatively charged cells and internalization remains low.…”

Section: Cellular Internalization and Exocytosis Of Nanodiamondsmentioning

confidence: 98%

“…While large aggregates cannot be easily internalized, smaller DNDs enter cells efficiently. Surface charge and modifications of NDs also influence the uptake (Weng et al 2012;Marcon et al 2010). Positively charged nanoparticles can easily penetrate the negatively charged cell membrane and are internalized rapidly.…”

Section: Cellular Internalization and Exocytosis Of Nanodiamondsmentioning

confidence: 99%

“…Their uptake is affected by many factors; the following paragraphs emphasize the most important ones. Particle size is a limiting factor for cellular internalization (Weng et al 2012;Lee et al 2013;Alhaddad et al 2012). While large aggregates cannot be easily internalized, smaller DNDs enter cells efficiently.…”

Section: Cellular Internalization and Exocytosis Of Nanodiamondsmentioning

confidence: 99%

See 4 more Smart Citations

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Šlegerová

Řehoř

Havlík

et al. 2014

Intracellular Delivery II

View full text Add to dashboard Cite

In recent years, diamond nanoparticles have received a great deal of attention due to their unique photophysical and biological properties. Nanodiamonds (NDs) show low toxicity and are considered to be a highly biocompatible carbon nanomaterial useful in a wide range of applications. Thanks to their ability to accommodate nitrogen-vacancy (N-V) color centers, NDs are a prime example of non-photobleachable fluorescent labels and nanosensors. Here, we present a survey of ND applications in biology and medicine with an emphasis on bioimaging. We focus on distinguishing the properties of detonation NDs and highpressure high-temperature (HPHT) NDs and describing their physicochemical properties, structure and possible modifications by small molecules and biomolecules. We summarize and critically evaluate in vitro and in vivo data on ND toxicity and biocompatibility, cellular internalization, localization and targeting by surface-attached ligands. We discuss current achievements in bioimaging using fluorescent NDs and the potential of NDs in diagnostics and drug delivery.

show abstract

Section: In Vitro Biocompatibility Of Hpht Ndsmentioning

confidence: 90%

Section: Biomolecules On the Nd Surfacementioning

confidence: 98%

Section: Cellular Internalization and Exocytosis Of Nanodiamondsmentioning

confidence: 98%

Section: Cellular Internalization and Exocytosis Of Nanodiamondsmentioning

confidence: 99%

Section: Cellular Internalization and Exocytosis Of Nanodiamondsmentioning

confidence: 99%

See 3 more Smart Citations

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Šlegerová

Řehoř

Havlík

et al. 2014

Intracellular Delivery II

View full text Add to dashboard Cite

show abstract

Plasmonic Nanodiamonds: Targeted Core–Shell Type Nanoparticles for Cancer Cell Thermoablation

Řehoř

Lee

Chen

et al. 2014

Adv Healthcare Materials

View full text Add to dashboard Cite

Targeted biocompatible nanostructures with controlled plasmonic and morphological parameters are promising materials for cancer treatment based on selective thermal ablation of cells. Here, core-shell plasmonic nanodiamonds consisting of a silica-encapsulated diamond nanocrystal coated in a gold shell is designed and synthesized. The architecture of particles is analyzed and confirmed in detail using 3-dimensional transmission electron microscope tomography. The particles are biocompatibilized using a PEG polymer terminated with bioorthogonally reactive alkyne groups. Azide-modified transferrin is attached to these particles, and their high colloidal stability and successful targeting to cancer cells overexpressing the transferrin receptor is demonstrated. The particles are nontoxic to the cells and they are readily internalized upon binding to the transferrin receptor. The high plasmonic cross section of the particles in the near-infrared region is utilized to quantitatively ablate the cancer cells with a short, one-minute irradiation by a pulse 750-nm laser.

show abstract

Cell Uptake of Lipid‐Coated Diamond

Morita

Martínez

Chipaux

et al. 2019

Part & Part Syst Charact

View full text Add to dashboard Cite

Fluorescent nanodiamonds (FNDs) can be used as nanoscale magnetic resonance sensors and stable optical labels. As a first step for using FNDs as nanosensors inside cells, they have to be ingested. Several techniques that improve particle uptake have been used. A simple approach based on commercially available liposomes is used to improve uptake. Uptake into colon cancer cells (HT‐29 cells) is demonstrated. Additionally, it is shown for the first time that one can facilitate diamond uptake into yeast cells by removing the cell wall and creating a so‐called spheroplast. Finally, the characteristics of FNDs coated with lipids and their behavior inside the cells are evaluated.

show abstract

Cellular uptake and phototoxicity of surface-modified fluorescent nanodiamonds

Cited by 29 publications

References 60 publications

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Nanodiamonds as Intracellular Probes for Imaging in Biology and Medicine

Plasmonic Nanodiamonds: Targeted Core–Shell Type Nanoparticles for Cancer Cell Thermoablation

Cell Uptake of Lipid‐Coated Diamond

Contact Info

Product

Resources

About