Cell Uptake of Lipid‐Coated Diamond

Morita, Aryan; Martínez, Felipe Perona; Chipaux, Mayeul; Jamot, Nicolas; Hemelaar, Simon R.; Laan, Kiran J. van der; Schirhagl, Romana

doi:10.1002/ppsc.201900116

Cited by 12 publications

(28 citation statements)

References 40 publications

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“…This kit contains 63 µmol L-α-phosphatidylcholine and 18 µmol stearylamine. After the coating process, the zeta potential value of FNDs becomes electropositive (36 ± 3 mV) [18]. To prepare FNDs coated with lipids (FND-lip), 2 µg mL −1 of FND solution was added into liposomes and was mixed by vortexing for 30 s.…”

Section: Coated Particlesmentioning

confidence: 99%

“…Characterization of diamond and lipid-coated diamond particles (FND-lip) has been performed in a previous study [18]. There we characterized several properties for these particles.…”

Section: Particle Characterizationmentioning

confidence: 99%

“…The zeta potential measurement showed that the 70 nm FNDs were electronegative (−15.73 ± 0.89 mV). After adding liposome, the particles became electropositive (35.67 ± 2.64 mV) [18]. Cryo TEM (recorded with Tecnai, Oregon, USA) revealed that the thickness of the lipid layer on diamond particles was 4.8 ± 1.2 nm [18].…”

Section: Particle Characterizationmentioning

confidence: 99%

“…Another option, which we used here, is to remove the cell wall entirely. This method allows the diamonds to enter and regrow the cell wall [18]. Here, we investigate for the first time what happens during and after cell division in yeast.…”

Section: Introductionmentioning

confidence: 99%

“…Cell division: The goal of this article is to determine which one of these possibilities is the case for nanodiamonds. To answer this question, we used FNDs in yeast cells using the spheroplasting process [18,24] (i.e., removing the cell wall) and we followed them during cell division. We investigated here which of the four possible outcomes occur and in what frequency.…”

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confidence: 99%

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The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast

et al. 2020

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Fluorescent nanodiamonds are frequently used as biolabels. They have also recently been established for magnetic resonance and temperature sensing at the nanoscale level. To properly use them in cell biology, we first have to understand their intracellular fate. Here, we investigated, for the first time, what happens to diamond particles during and after cell division in yeast (Saccharomyces cerevisiae) cells. More concretely, our goal was to answer the question of whether nanodiamonds remain in the mother cells or end up in the daughter cells. Yeast cells are widely used as a model organism in aging and biotechnology research, and they are particularly interesting because their asymmetric cell division leads to morphologically different mother and daughter cells. Although yeast cells have a mechanism to prevent potentially harmful substances from entering the daughter cells, we found an increased number of diamond particles in daughter cells. Additionally, we found substantial excretion of particles, which has not been reported for mammalian cells. We also investigated what types of movement diamond particles undergo in the cells. Finally, we also compared bare nanodiamonds with lipid-coated diamonds, and there were no significant differences in respect to either movement or intracellular fate.

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Section: Coated Particlesmentioning

confidence: 99%

“…Characterization of diamond and lipid-coated diamond particles (FND-lip) has been performed in a previous study [18]. There we characterized several properties for these particles.…”

Section: Particle Characterizationmentioning

confidence: 99%

Section: Particle Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast

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Diamond‐Based Nanoscale Quantum Relaxometry for Sensing Free Radical Production in Cells

Sigaeva

Shirzad

Martínez

et al. 2022

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≈10 12 -10 18 nuclei (or a factor of 1000 fewer electron spins) to generate an observable signal. [6,7] To date, diamond magnetometry has been used to detect magnetic structures, [8][9][10][11] microwave signals, [12] and single proteins [13] and to record signals from magnetotactic bacteria, [14] cells labeled with magnetic particles, [15] or fixed and stained cell slices. [16] Relaxometry (or T 1 ), analogous to T 1 in conventional MRI, is a quantum sensing method that allows the efficient detection of surrounding magnetic noise. [17] First with gadolinium contrast, [18][19][20] it has been used in solution to detect free radicals [21] and monitor chemical reactions, [22,23] and in biological environments to sense the metabolic activity of mitochondria in macrophages [24] or detect nitric oxide production by endothelial cells. [25] Conventional methods for detecting radicals rely on chemical reactions with spin labels or dye molecules and on detection via electron spin resonance. The first generally requires large sample volumes, [26] and the second is poorly selective for free radicals, irreversibly consumes them, and is prone to bleaching, which complicates real-time monitoring. [27] Here, we detected nitric oxide signaling at the nanoscale with an optical resolution. In the human body, nitric oxide is particularly relevant for vascular and brain cell communication and therefore essential for understanding heart and brain diseases. [28,29] We were able to quantify and localize nitric oxide, formed either chemically or by live mammalian cells. Additionally, we determined the formation dynamics, which are inaccessible by state-of-the-art methods. Results and DiscussionAs evidenced previously, the relaxometry measurement sequence allows the detection of free radicals via the magnetic noise they generate. [18] In this work, we used this technique to localize the generation of biologically relevant nitric oxide and superoxide. Toward this goal, we conducted relaxometry measurements using nanodiamonds containing ensembles of nitrogen-vacancy (NV) centers. Such nanodiamonds can be internalized by different types of cells [30][31][32][33] and exhibit excellent biocompatibility. [34,35] In our experiments, the Diamond magnetometry makes use of fluorescent defects in diamonds to convert magnetic resonance signals into fluorescence. Because optical photons can be detected much more sensitively, this technique currently holds several sensitivity world records for room temperature magnetic measurements. It is orders of magnitude more sensitive than conventional magnetic resonance imaging (MRI) for detecting magnetic resonances. Here, the use of diamond magnetometry to detect free radical production in single living cells with nanometer resolution is experimentally demonstrated. This measuring system is first optimized and calibrated with chemicals at known concentrations. These measurements serve as benchmarks for future experiments. While conventional MRI typically has millimeter resolution, measurements are performed on...

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Detecting the metabolism of individual yeast mutant strain cells when aged, stressed or treated with antioxidants with diamond magnetometry

et al. 2023

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Cell Uptake of Lipid‐Coated Diamond

Cited by 12 publications

References 40 publications

The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast

The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast

Diamond‐Based Nanoscale Quantum Relaxometry for Sensing Free Radical Production in Cells

Detecting the metabolism of individual yeast mutant strain cells when aged, stressed or treated with antioxidants with diamond magnetometry

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