Cellular Transplantation and Spinal Cord Injury

Barami, Kaveh; Díaz, Fernando G.

doi:10.1227/00006123-200009000-00033

Cited by 15 publications

(24 citation statements)

References 60 publications

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“…1,2 Traumatic SCI can have several devastating consequences, such as chronic pain and paralysis. 1,2 Traumatic SCI can have several devastating consequences, such as chronic pain and paralysis.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Downregulation of long noncoding RNA Sox2ot protects PC‐12 cells from hydrogen peroxide–induced injury in spinal cord injury via regulating the miR‐211‐myeloid cell leukemia‐1 isoform2 axis

Yin

Zheng

Zhuang

et al. 2018

J of Cellular Biochemistry

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This study aimed to investigate the effects and possible mechanisms of long noncoding RNA (lncRNA) Sox2 overlapping transcript (Sox2ot) on hydrogen peroxide (H O )-induced injury in pheochromocytoma (PC-12) cells. PC-12 cells were treated with H O to cell injury. The cells were transfected with short-hairpin RNA directed against Sox2ot (sh-Sox2ot), small interfering RNA directed against myeloid cell leukemia-1 (MCL-1) isoform2 (si-MCL-1), a miR-211 mimic, a miR-211 inhibitor, and their negative controls. Under different transfected treatments, cell viability, migration, invasion, and apoptosis as well as the expressions of apoptosis- and autophagy-related proteins were investigated. Besides, the regulatory relationships between Sox2ot and miR-211, miR-211 and MCL-1, as well as between MCL-1 and the protein kinase B (Akt)/mammalian target of the rapamycin (mTOR)/p70 ribosomal S6 protein kinase (p70S6K) signaling pathway were explored. Suppression of Sox2ot inhibited H O -induced PC-12 cell injury by increasing cell viability, migration, invasion, and decreasing apoptosis and autophagy. Moreover, suppression of Sox2ot increased miR-211 expression and alleviated H O -induced injury in PC-12 cells possibly via upregulation of miR-211. Furthermore, MCL-1 isoform2 was identified as a direct target of miR-211 and could be negatively regulated by miR-211. Suppression of miR-211 aggravated H O -induced cell injury by regulation of MCL-1 isoform2. Besides, inhibition of miR-211 suppressed the activation of the Akt/mTOR/p70S6K signaling pathway in H O -treated PC-12 cells, which was reversed after knockdown of MCL-1 isoform2 at the same time. Our findings indicate that downregulation of Sox2ot may protect PC-12 cells from H O -induced injury in SCI via targeting the miR-211/MCL-1 isoform2 axis. MCL-1 isoform2 may further regulate the activation of the Akt/mTOR/p70S6K pathway to mediate H O -induced injury. The Sox2ot-miR-211-MCL-1 isoform2 axis may be a promising therapeutic strategy for SCI.

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Section: Introductionmentioning

confidence: 99%

Retracted: Downregulation of long noncoding RNA Sox2ot protects PC‐12 cells from hydrogen peroxide–induced injury in spinal cord injury via regulating the miR‐211‐myeloid cell leukemia‐1 isoform2 axis

Yin

Zheng

Zhuang

et al. 2018

J of Cellular Biochemistry

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“…[64][65][66][67] Macrophages Recruitment of macrophages is limited in CNS and the resident microglia cells are the main immune cells that are activated after spinal cord injury. [68] It has been shown that by controlled boosting of local immune response by delivering autologous macrophages, one can promote recovery in spinal cord injury. [69] The possible mechanisms are activation of infiltrating T cells and increased production of trophic factors and brainderived neurotrophic factor, [70,71] thus leading to removal of inhibitory myelin debris.…”

Section: The Use Of Human Umbilical Cord Blood a Rich Source Of Nonementioning

confidence: 99%

Pluripotent stem cells - A review of the current status in neural regeneration

et al. 2011

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Pharmacological or neurosurgical therapies currently in practice to treat the damage in various neurodegenerative disorders are not efficient in preventing progression or cure of these progressive neurodegenerative processes. Recently, a new approach, cell therapy using stem cell, is being evaluated. However, the use of this therapy in the treatment of these neurological diseases is highly restricted, mainly owing to several technical difficulties and limitations. The strategy of isolation and characterization of neural stem cells from various sources will probably provide a major impetus and open up an interesting, novel therapeutic modality for several neurodegenerative disorders. The high regenerative potential of damaged neural tissues suggests that various embryonic/adult sources serve as a proxy for neural stem cells for cell-based therapy.

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“…49 Lee et al 34 radiologically studied the migration of implanted olfactory ensheathing cells into the rat spinal cord. Intralesional gliotic tissue, in patients with chronic cord injury, is another mechanical obstacle, which hinders the formation of cellular bridges within the damaged area of the cord by the transplanted stem cells.…”

Section: Intralesional Gliosismentioning

confidence: 99%

Feasibility, Safety, and Efficacy of Directly Transplanting Autologous Adult Bone Marrow Stem Cells in Patients With Chronic Traumatic Dorsal Cord Injury

Abdelaziz

Marie

Abbas

et al. 2010

Neurosurgery Quarterly

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Background: Bone marrow is a readily accessible source for autologous adult bone marrow stem cells which can be applied therapeutically without possessing the risk of immune rejection and without raising ethical concerns. The purpose of this study is to determine the feasibility, safety, and effectiveness of direct transplantation of autologous adult bone marrow stem cells in patients with chronic cord injuries.Methods: Thirty consecutive patients (5 females and 25 males, aged 6 to 64 y) having chronic traumatic dorsal spinal cord injury with durations of at least 6 months were included in the study. Twenty patients were treated with autologous adult bone marrow stem cells transplantation through open surgical intraparenchymal and intralesional injection into the site of cord injury. The treatment was continued with monthly intrathecal injection of stem cells through lumbar or cisternal punctures. Ten other patients were not treated with stem cells and served as control cases.Results: Clinical improvement was observed in 6 (30%) of 20 patients treated with stem cells transplantation. Short duration of injury and small cord lesions correlated with good outcome. Follow-up electrophysiologic studies did not show statistically significant changes. Follow-up magnetic resonance imaging did not show significant changes. Minor and temporary treatmentrelated morbidity were recorded.Conclusions: The application of autologous adult bone marrow mesenchymal stem cells directly into the spinal cord is relatively safe and has clinical benefits in patients with chronic spinal cord injury. However, multicenter studies should be conducted to further elucidate the safety and efficacy of stem cells therapy in patients with spinal cord injury.

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Cellular Transplantation and Spinal Cord Injury

Cited by 15 publications

References 60 publications

Retracted: Downregulation of long noncoding RNA Sox2ot protects PC‐12 cells from hydrogen peroxide–induced injury in spinal cord injury via regulating the miR‐211‐myeloid cell leukemia‐1 isoform2 axis

Retracted: Downregulation of long noncoding RNA Sox2ot protects PC‐12 cells from hydrogen peroxide–induced injury in spinal cord injury via regulating the miR‐211‐myeloid cell leukemia‐1 isoform2 axis

Pluripotent stem cells - A review of the current status in neural regeneration

Feasibility, Safety, and Efficacy of Directly Transplanting Autologous Adult Bone Marrow Stem Cells in Patients With Chronic Traumatic Dorsal Cord Injury

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