Cellular transcriptional response to zirconia-based implant materials

Altmann, Brigitte; Rabel, Kerstin; Kohal, Ralf‐Joachim; Proksch, Susanne; Tomakidi, Pascal; Adolfsson, Erik; Bernsmann, Falk; Palmero, Paola; Fürderer, Tobias; Steinberg, Thorsten

doi:10.1016/j.dental.2016.12.005

Cited by 18 publications

(15 citation statements)

References 46 publications

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“…Investigations have suggested that zirconia surface modification influences on osteoblast and fibroblasts adhesion, proliferation, morphology, and differentiation leading to the osseointegration of implant surfaces and fit to the soft peri-implant tissue [29,30]. The understanding of surface physicochemical modifications on biocompatibility and osseointegration measured by histomorphometric analyses have been copiously evaluated to predict the clinical outcome and peri-implant tissue stability over time [31,32].…”

Section: Resultsmentioning

confidence: 99%

Zirconia surface modifications for implant dentistry

Schünemann

Galárraga-Vinueza

Magini

et al. 2019

Materials Science and Engineering: C

224

144

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Background: Zirconia has emerged as a versatile dental material due to its excellent aesthetic outcomes such as color and opacity, unique mechanical properties that can mimic the appearance of natural teeth and decrease peri-implant inflammatory reactions. Objective: The aim of this review was to critically explore the state of art of zirconia surface treatment to enhance its biological and osseointegration behavior in implant dentistry. Materials and Methods: An electronic search in PubMed database was carried out until May 2018 using the following combination of key words and MeSH terms without time periods: "zirconia surface treatment" or "zirconia surface modification" or "zirconia coating" and "osseointegration" or "biological properties" or "bioactivity" or "functionally graded properties". Results: Previous studies have reported the influence of zirconia-based implant surface on the adhesion, proliferation, and differentiation of osteoblast and fibroblasts at the implant to bone interface during the osseointegration process. A large number of physicochemical methods have been used to change the implant surfaces to improve the early and late bone-to-implant integration, namely: acid etching, gritblasting, laser treatment, UV light, CVD, and PVD. The development of coatings composed of silica, magnesium, graphene, dopamine, and bioactive molecules has been assessed although the development of a functionally graded material for implants has shown encouraging mechanical and biological behavior.

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Section: Resultsmentioning

confidence: 99%

Zirconia surface modifications for implant dentistry

Schünemann

Galárraga-Vinueza

Magini

et al. 2019

Materials Science and Engineering: C

224

144

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“…The Alizarin red stains intracellular calcium deposits and calcium bound to proteins and proteoglycans 55 , 56 . This dye was used to analyse the deposition of mineralized matrix by osteoblasts, which is a marker of cell maturation.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing

Souza

Lopes

Encarnação

et al. 2018

Sci Rep

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The present work presents and discusses the results of a comprehensive study on the bioactive properties of Nb-substituted silicate glass derived from 45S5 bioglass. In vitro and in vivo experiments were performed. We undertook three different types of in vitro analyses: (i) investigation of the kinetics of chemical reactivity and the bioactivity of Nb-substituted glass in simulated body fluid (SBF) by 31P MASNMR spectroscopy, (ii) determination of ionic leaching profiles in buffered solution by inductively coupled plasma optical emission spectrometry (ICP-OES), and (iii) assessment of the compatibility and osteogenic differentiation of human embryonic stem cells (hESCs) treated with dissolution products of different compositions of Nb-substituted glass. The results revealed that Nb-substituted glass is not toxic to hESCs. Moreover, adding up to 1.3 mol% of Nb2O5 to 45S5 bioglass significantly enhanced its osteogenic capacity. For the in vivo experiments, trial glass rods were implanted into circular defects in rat tibia in order to evaluate their biocompatibility and bioactivity. Results showed all Nb-containing glass was biocompatible and that the addition of 1.3 mol% of Nb2O5, replacing phosphorous, increases the osteostimulation of bioglass. Therefore, these results support the assertion that Nb-substituted glass is suitable for biomedical applications.

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“…39,52 There has been a considerable interest and effort in improving zirconia surfaces, particularly the development of surface roughening techniques. Physical, [53][54][55] chemical, [54][55][56] and thermochemical 57 modifications of zirconia surfaces successfully improved cellular response and function. However, roughness parameters reported for zirconia implants are generally lower than those for titanium implants.…”

Section: Introductionmentioning

confidence: 99%

Biological and osseointegration capabilities of hierarchically (meso-/micro-/nano-scale) roughened zirconia

Rezaei¹,

Hasegawa²,

Ishijima³

et al. 2018

IJN

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PurposeZirconia is a potential alternative to titanium for dental and orthopedic implants. Here we report the biological and bone integration capabilities of a new zirconia surface with distinct morphology at the meso-, micro-, and nano-scales.MethodsMachine-smooth and roughened zirconia disks were prepared from yttria-stabilized tetragonal zirconia polycrystal (Y-TZP), with rough zirconia created by solid-state laser sculpting. Morphology of the surfaces was analyzed by three-dimensional imaging and profiling. Rat femur-derived bone marrow cells were cultured on zirconia disks. Zirconia implants were placed in rat femurs and the strength of osseointegration was evaluated by biomechanical push-in test.ResultsThe rough zirconia surface was characterized by meso-scale (50 µm wide, 6–8 µm deep) grooves, micro-scale (1–10 µm wide, 0.1–3 µm deep) valleys, and nano-scale (10–400 nm wide, 10–300 nm high) nodules, whereas the machined surface was flat and uniform. The average roughness (Ra) of rough zirconia was five times greater than that of machined zirconia. The expression of bone-related genes such as collagen I, osteopontin, osteocalcin, and BMP-2 was 7–25 times upregulated in osteoblasts on rough zirconia at the early stage of culture. The number of attached cells and rate of proliferation were similar between machined and rough zirconia. The strength of osseointegration for rough zirconia was twice that of machined zirconia at weeks two and four of healing, with evidence of mineralized tissue persisting around rough zirconia implants as visualized by electron microscopy and elemental analysis.ConclusionThis unique meso-/micro-/nano-scale rough zirconia showed a remarkable increase in osseointegration compared to machine-smooth zirconia associated with accelerated differentiation of osteoblasts. Cell attachment and proliferation were not compromised on rough zirconia unlike on rough titanium. This is the first report introducing a rough zirconia surface with distinct hierarchical morphology and providing an effective strategy to improve and develop zirconia implants.

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Cellular transcriptional response to zirconia-based implant materials

Cited by 18 publications

References 46 publications

Zirconia surface modifications for implant dentistry

Zirconia surface modifications for implant dentistry

Comprehensive in vitro and in vivo studies of novel melt-derived Nb-substituted 45S5 bioglass reveal its enhanced bioactive properties for bone healing

Biological and osseointegration capabilities of hierarchically (meso-/micro-/nano-scale) roughened zirconia

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