Cellular self-organization on micro-structured surfaces

Röttgermann, Peter J. F.; Alberola, Alicia Piera; Rädler, Joachim O.

doi:10.1039/c3sm52419a

Cited by 22 publications

(24 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of cells captured on microfluidic- [24] or micro-patterned cell arrays offers a route towards high-throughput analysis. We recently introduced micro-patterned substrates for time-resolved measurements on regularly arrayed cells, and showed that cells self-organize onto fibronectin-coated sites surrounded by boundaries passivated by treatment with poly- l -lysine- polyethylene glycol [25,26]. …”

Section: Introductionmentioning

confidence: 99%

Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

2016

View full text Add to dashboard Cite

Cell fate decisions like apoptosis are heterogeneously implemented within a cell population and, consequently, the population response is recognized as sum of many individual dynamic events. Here, we report on the use of micro-patterned single-cell arrays for real-time tracking of nanoparticle-induced (NP) cell death in sets of thousands of cells in parallel. Annexin (pSIVA) and propidium iodide (PI), two fluorescent indicators of apoptosis, are simultaneously monitored after exposure to functionalized polystyrene (PS−NH2) nanobeads as a model system. We find that the distribution of Annexin onset times shifts to later times and broadens as a function of decreasing NP dose. We discuss the mean time-to-death as a function of dose, and show how the EC50 value depends both on dose and time of measurement. In addition, the correlations between the early and late apoptotic markers indicate a systematic shift from apoptotic towards necrotic cell death during the course of the experiment. Thus, our work demonstrates the potential of array-based single cell cytometry for kinetic analysis of signaling cascades in a high-throughput format.

show abstract

Section: Introductionmentioning

confidence: 99%

Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

2016

View full text Add to dashboard Cite

show abstract

“…Wound healing is a typical biological assay to study collective migration of cells under controlled conditions in vitro and is a prototypical experimental method to study active matter (7)(8)(9)(10). Experiments performed on soluble collagen (11) or other gels (12), micropatterned (13,14) and deformable substrates (1) show that cell migration is guided by the substrate structure and stiffness (5,15,16).…”

mentioning

confidence: 99%

Bursts of activity in collective cell migration

Chepizhko

Giampietro

Mastrapasqua

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Dense monolayers of living cells display intriguing relaxation dynamics, reminiscent of soft and glassy materials close to the jamming transition, and migrate collectively when space is available, as in wound healing or in cancer invasion. Here we show that collective cell migration occurs in bursts that are similar to those recorded in the propagation of cracks, fluid fronts in porous media, and ferromagnetic domain walls. In analogy with these systems, the distribution of activity bursts displays scaling laws that are universal in different cell types and for cells moving on different substrates. The main features of the invasion dynamics are quantitatively captured by a model of interacting active particles moving in a disordered landscape. Our results illustrate that collective motion of living cells is analogous to the corresponding dynamics in driven, but inanimate, systems.collective cell migration | avalanches | universality | vascular endothelial cadherin | collagen substrate C ollective cell movement depends on intracellular biological mechanisms as well as environmental cues due to the extracellular matrix (1-5), mainly composed of collagen which is organized in hierarchical structures, such as fibrils and fibers. The mechanical properties of collagen fibril networks are essential to offer little resistance and high sensitivity to small deformations, allowing easy local remodeling and strong strain stiffening needed to ensure cell and tissue integrity (6). Wound healing is a typical biological assay to study collective migration of cells under controlled conditions in vitro and is a prototypical experimental method to study active matter (7-10). Experiments performed on soluble collagen (11) or other gels (12), micropatterned (13, 14) and deformable substrates (1) show that cell migration is guided by the substrate structure and stiffness (5,15,16).It has been argued that collective migration properties arise from stresses transmitted between neighboring cells (1) giving rise to longranged stress waves in the monolayer (17,18). Hence the dynamics of an invading cell sheet is ruled by a combination of long-range internal stresses and interactions with the substrate, suggesting an analogy with driven elastic systems moving in a disordered medium such as cracks lines (19,20), imbibition fronts (21), or ferromagnetic domain walls (22). The scaling laws in these systems are usually associated with a depinning critical point that has been widely studied by simple models for interface dynamics. Thanks to a combination of numerical simulations (23, 24) and renormalization group theory (23,(25)(26)(27), we now have a detailed picture of the nonequilibrium phase transitions and universality classes in these systems. Here we substantiate the analogy between collective cell migration and depinning by revealing and characterizing widely distributed bursts of activity in the collective migration of different types of cells (human cancer cells and epithelial cells, mouse endothelial cells) over different substrates ...

show abstract

“…In order to test whether the patterns produced are suitable for cell confinement over long timescales, we carried out time-lapse measurements of cells on patterned surfaces over extended time periods. As shown in Fig.S3 of the Supplementary Material (and seen in earlier studies 7,10,39 ), the patterns are stable and capable of confining cells over periods of up to several days.…”

Section: Resultsmentioning

confidence: 87%

Versatile method to generate multiple types of micropatterns

et al. 2016

View full text Add to dashboard Cite

Micropatterning techniques have become an important tool for the study of cell behavior in controlled microenvironments. As a consequence, several approaches for the creation of micropatterns have been developed in recent years. However, the diversity of substrates, coatings and complex patterns used in cell science is so great that no single existing technique is capable of fabricating designs suitable for all experimental conditions. Hence, there is a need for patterning protocols that are flexible with regard to the materials used and compatible with different patterning strategies to create more elaborate setups. In this work, we present a versatile approach to micropatterning. The protocol is based on plasma treatment, protein coating, and a PLL-PEG backfill step, and produces homogeneous patterns on a variety of substrates. Protein density within the patterns can be controlled, and density gradients of surface-bound protein can be formed. Moreover, by combining the method with microcontact printing, it is possible to generate patterns composed of three different components within one iteration of the protocol. The technique is simple to implement and should enable cell science labs to create a broad range of complex and highly specialized microenvironments.

show abstract

Cellular self-organization on micro-structured surfaces

Cited by 22 publications

References 43 publications

Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

Time-Resolved Study of Nanoparticle Induced Apoptosis Using Microfabricated Single Cell Arrays

Bursts of activity in collective cell migration

Versatile method to generate multiple types of micropatterns

Contact Info

Product

Resources

About