Plasticity of cancer invasion and metastasis depends on the ability of cancer cells to switch between collective and single cell dissemination, controlled by cadherinmediated cell-cell junctions. In clinical samples, E-cadherin expressing and deficient tumors both invade collectively and metastasize equally, implicating additional mechanisms controlling cell-cell cooperation and individualization. Using spatially defined organotypic culture, intravital microscopy of mammary tumors in mice and in silico modeling, we here identify cell density regulation by 3D tissue boundaries to physically control collective movement irrespective of the composition and stability of cell-cell junctions. Deregulation of adherens junctions, including E-cadherin and p120-catenin, resulted in a transition from coordinated to uncoordinated collective movement along extracellular boundaries, whereas singlecell escape depended on locally free tissue space. These data indicate that cadherins and ECM confinement cooperate to determine unjamming transitions towards step-wise epithelial fluidization and, ultimately, cell individualization.

Coherent vortical motion has been reported in a wide variety of populations including living organisms (bacteria, fishes, human crowds) and synthetic active matter (shaken grains, mixtures of biopolymers), yet a unified description of the formation and structure of this pattern remains lacking. Here we report the self-organization of motile colloids into a macroscopic steadily rotating vortex. Combining physical experiments and numerical simulations, we elucidate this collective behaviour. We demonstrate that the emergent-vortex structure lives on the verge of a phase separation, and single out the very constituents responsible for this state of polar active matter. Building on this observation, we establish a continuum theory and lay out a strong foundation for the description of vortical collective motion in a broad class of motile populations constrained by geometrical boundaries.

We study the transport properties of a system of active particles moving at constant speed in a heterogeneous two-dimensional space. The spatial heterogeneity is modeled by a random distribution of obstacles, which the active particles avoid. Obstacle avoidance is characterized by the particle turning speed γ. We show, through simulations and analytical calculations, that the mean square displacement of particles exhibits two regimes as function of the density of obstacles ρ(o) and γ. We find that at low values of γ, particle motion is diffusive and characterized by a diffusion coefficient that displays a minimum at an intermediate obstacle density ρ(o). We observe that in high obstacle density regions and for large γ values, spontaneous trapping of active particles occurs. We show that such trapping leads to genuine subdiffusive motion of the active particles. We indicate how these findings can be used to fabricate a filter of active particles.