Cellular Responses of MC3T3-E1 Mouse Osteoblast-Like Cells to Rough Surfaced Substrates

Abstract

MC3T3-E1 mouse osteoblast-like cells were seeded at high cell density to form confluent monolayer on rough surfaced culture substrates. Osteoblastic gene and protein expressions and matrix mineralization were investigated to clarify the effect of surface roughness on differentiation of MC3T3-E1 cells.