Cellular Responses and Proteomic Analysis of Escherichia coli Exposed to Green Tea Polyphenols

Cho, Y. S.; Schiller, Neal L.; Kahng, Hyung-Yeel; Oh, Keun-Yeob

doi:10.1007/s00284-007-9021-8

Cited by 72 publications

(48 citation statements)

References 19 publications

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“…On the other hand, Ikigai et al reported that catechins primarily act on and damage bacterial membranes [13]. In a recent study, we reported that TPP-treated E. coli had unique changes in saturated and unsaturated fatty acids in their cell membranes, further supporting the impact of TPP on membranes [6]. These studies illustrate that TPP may have multiple mechanisms of antibacterial activity, which can synergize the bactericidal activity of various antibiotics.…”

Section: Resultsmentioning

confidence: 72%

“…Recently, we investigated the antibacterial effects of TPP on E. coli as well as proteomic changes induced by exposure to TPP [6]. In the present study, we examined the antibacterial and synergistic activities of TPP on 13 clinical isolates of MRSA, and comparative proteomic analysis was performed to elucidate the proteins expressed in MRSA exposed to TPP.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Effects of Green Tea Polyphenols on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Cho

Schiller

2008

Curr Microbiol

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The antibacterial effects of tea polyphenols (TPP) extracted from Korean green tea (Camellia sinensis) against clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were evaluated. Characterization of the minimal inhibitory concentration (MIC) of oxacillin for 30 S. aureus strains isolated from patients treated with oxacillin identified 13 strains with an oxacillin MIC C 4 lg/mL as methicillinresistant Staphylococcus aureus (MRSA) (range: 8 to 512 lg/ mL), while 17 strains were methicillin-susceptible Staphylococcus aureus (MSSA) (range: 0.25-0.5 lg/mL). The MICs of TPP ranged from 50 to 180 lg/mL for both the MSSA and the MRSA strains. The MICs of oxacillin for each of the 13 MRSA strains were reduced between 8-and 128-fold when these strains were coincubated with sub-MIC (B0.59 MIC) levels of TPP, demonstrating that the combination of TPP plus oxacillin was synergistic for all of the clinical MRSA isolates. Two-dimensional polyacrylamide gel electrophoresis identified 14 extracellular proteins of MRSA-13 down-regulated and 3 proteins up-regulated by exposure to TPP. These studies demonstrate that TPP can differentially stimulate the expression of various proteins in these bacteria and synergize the bactericidal activity of oxacillin for MRSA.

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Section: Resultsmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Antibacterial Effects of Green Tea Polyphenols on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Cho

Schiller

2008

Curr Microbiol

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“…It has also been shown that certain extracts of antioxidant tea inhibitors inhibit some food-borne pathogenic microorganisms such as Bacillus cereus (Almajano et al, 2008;Si et al, 2006), Staphylococcus aureus Si et al, 2006;Taguri et al, 2004), Vibrio cholerae (Taguri et al, 2004), Listeria monocytogenes Si et al, 2006), Escherichia coli (Cho et al, 2007;Si et al, 2006), Salmonella typhimurium (Si et al, 2006), Salmonella enteritidis , Micrococcus luteus y Pseudomona aeruginosa (Almajano et al, 2008); and that in particular green tea catechins may decrease the resistance of S. aureus to antibiotics such as oxacillin (Stapleton et al, 2004).…”

Section: Antifungal Activitymentioning

confidence: 99%

Biological and antioxidant activity of Gunnera tinctoria (Nalca)

Zamorano¹,

Rojano²,

Morales³

et al. 2017

J. Med. Plants Res.

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The endemic Chilean edible plant Gunnera tinctoria (Nalca) is highly appreciated in the south of Chile by the small farmers. Nevertheless, no background exists about his secondary metabolites. In the present study, in the leaf from G. tinctoria was investigated the content of bioactive compounds like coumaric acid, ascorbic acid and total phenols; the antioxidant capacity was evaluated by 3-ethylbenzothiazoline-6-suslfonic acid (ABTS), oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods reducing sugars were measured. Finally, the biological activity was evaluated against Cladophialophora and Cryptococcus laurentii. The results suggest that the most abundant constituent in the extract were catechin (1344.97 mg/100 g dry weight) and epicatechin (1429.28 mg/100g dry weight), and was confirmed and quantified by high performance liquid chromatografy (HPLC-PDA); while the ORAC methodology showed a high antioxidant capacity (192000.0±5.91 umol Trolox Eq/100 g dry weight). On the other hand, the extract had a fungicide effect against both microorganism assayed, inhibiting the growth of Cladophialophora´s mold-and the yeast Cryptococcus laurentii. This is the first report of antioxidant capacity, bioactive compounds and biological activity of G. tinctoria, and these findings suggest that an extract prepared from the Nalca leaf may be a promising source of antioxidant and bioactive compounds and as a research object by being an antifungal and therapeutic alternative in development.

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“…Proteomic analyses have increased our understanding of chemical-induced stress proteins in bacteria such as Acinetobater, Burkholderia, Pseudomonas, and Escherichia [5][6][7][8]. Recently, we investigated the antibacterial effects of green tea polyphenols (TPP) on methicillin-resistant Staphylococcus aureus (MRSA) and E. coli and the proteomic changes induced by exposure to TPP in these bacteria [9,10]. Although there have been several reports that evaluated the antibacterial effects of the rose flower extract against several bacteria [11,12], we are unaware of any published studies on the cellular and proteomic responses of clinical isolates exposed to the rose flower extract.…”

Section: Introductionmentioning

confidence: 99%

Cellular and proteomic responses of Escherichia coli JK-17 exposed to the Rosa hybrida flower extract

Cho

2011

Biotechnol Bioproc E

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The purpose of this work was to characterize the cellular and proteomic responses of Escherichia coli JK-17 exposed to the rose flower extract (Rosa hybrida). The bacterial isolate was enriched and isolated from contaminated food. 16S rRNA sequence analyses revealed that the strain was 99% similar to the E. coli species cluster; therefore, this strain was designated E. coli JK-17. The rose flower extract showed a dose-dependent antibacterial effect on E. coli JK-17. Treatment of E. coli JK-17 with 50 and 100 mg/mL of the rose flower extract completely inhibited growth within 12 and 6 h of incubation. The stress shock proteins (SSPs) were induced with different concentrations of rose flower extract. The proteins were identified as 70-kDa DnaK and 60-kDa GroEL by SDS-PAGE and Western blot using anti-DnaK and antiGroEL monoclonal antibodies. The levels of SSPs induced by the rose flower extract increased when the exposure time to the rose flower extract was increased. SDS-PAGE with silver staining revealed that the amount of lipopolysaccharide (LPS) in E. coli JK-17 increased or decreased with different concentrations and exposure times of the rose flower extract. To identify proteins induced by the rose flower extract, 2-dimensional electrophoresis (2-DE) was applied to soluble protein fractions of E. coli JK-17 cultures. In the pH range of 4 ~ 7, more than 250 spots were detected on the silver stained gels. Notably, 15 protein spots were increased or decreased after treatment with the rose flower extract. Twelve up-regulated proteins were identified as chaperones (DnaK and GroEL) and porin proteins (PhoE, RfaI, RfaG, MdoH, and WzzE) by MALDI-TOF mass spectrometry, and three down-regulated proteins were identified, including proteins involved in energy and DNA metabolism (SdhA and GyrB), and amino acid biosynthesis (GltK). Using scanning electron microscopic analysis, some cells were shown to adopt irregular rod shapes and wrinkled surfaces after treatment with the rose flower extract. These results provide clues for better understanding the mechanism of rose flower extract-induced stress and cytotoxicity in E. coli JK-17.

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Cellular Responses and Proteomic Analysis of Escherichia coli Exposed to Green Tea Polyphenols

Cited by 72 publications

References 19 publications

Antibacterial Effects of Green Tea Polyphenols on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Antibacterial Effects of Green Tea Polyphenols on Clinical Isolates of Methicillin-Resistant Staphylococcus aureus

Biological and antioxidant activity of Gunnera tinctoria (Nalca)

Cellular and proteomic responses of Escherichia coli JK-17 exposed to the Rosa hybrida flower extract

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