Cellular resolution volumetric in vivo retinal imaging with adaptive optics–optical coherence tomography

Zawadzki, Robert J.; Choi, Stacey S.; Fuller, Alfred R.; Evans, Julia W.; Hamann, Bernd; Werner, John S.

doi:10.1364/oe.17.004084

Cited by 78 publications

(50 citation statements)

References 38 publications

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“…Due to the small field of view, 3D data sets are usually restricted to either the macula or the optical nerve head (ONH) area, and the operator needs to decide on the scanned region prior to data acquisition. Stitching or "mosaicing" of multiple data sets can be applied to increase the effective field of view, but this techniques increase acquisition time considerably [9][10][11]. Moreover, all the volumes need to be acquired before fusing them, which results in significant delay between capturing images and displaying large field of view.…”

Section: Importance Of Acquisition Speed In Retinal Octmentioning

confidence: 99%

“…Moreover, if the OCT imaging speed is increased up into the MHz and multi-MHz range, efficient speckle reduction by frame averaging can be used without loss of wide-field 3D and functional imaging capabilities, as will be shown in this paper. Other particularly promising applications of highspeed 3D OCT are the tomographic analysis of the posterior pole [24], volumetric adaptive optics OCT [9] and functional imaging [25][26][27][28][29]. For instance, the system configuration described in this paper has also been used to generate in-vivo single-shot wide-field microangiography images of the retina for the first time [30].…”

Section: Importance Of Acquisition Speed In Retinal Octmentioning

confidence: 99%

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Multi-MHz retinal OCT

Klein

Wieser

Reznicek

et al. 2013

Biomed. Opt. Express

199

124

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Abstract:We analyze the benefits and problems of in vivo optical coherence tomography (OCT) imaging of the human retina at A-scan rates in excess of 1 MHz, using a 1050 nm Fourier-domain mode-locked (FDML) laser. Different scanning strategies enabled by MHz OCT line rates are investigated, and a simple multi-volume data processing approach is presented. In-vivo OCT of the human ocular fundus is performed at different axial scan rates of up to 6.7 MHz. High quality non-mydriatic retinal imaging over an ultra-wide field is achieved by a combination of several key improvements compared to previous setups. For the FDML laser, long coherence lengths and 72 nm wavelength tuning range are achieved using a chirped fiber Bragg grating in a laser cavity at 419.1 kHz fundamental tuning rate. Very large data sets can be acquired with sustained data transfer from the data acquisition card to host computer memory, enabling high-quality averaging of many frames and of multiple aligned data sets. Three imaging modes are investigated: Alignment and averaging of 24 data sets at 1.68 MHz axial line rate, ultra-dense transverse sampling at 3.35 MHz line rate, and dual-beam imaging with two laser spots on the retina at an effective line rate of 6.7 MHz. 58. R. Huber, D. C. Adler, V. J. Srinivasan, and J. G. Fujimoto, "Fourier domain mode locking at 1050 nm for ultrahigh-speed optical coherence tomography of the human retina at 236,000 axial scans per second," Opt. Lett. ©2013 Optical Society of America

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Section: Importance Of Acquisition Speed In Retinal Octmentioning

confidence: 99%

Section: Importance Of Acquisition Speed In Retinal Octmentioning

confidence: 99%

Multi-MHz retinal OCT

Klein

Wieser

Reznicek

et al. 2013

Biomed. Opt. Express

199

124

View full text Add to dashboard Cite

show abstract

“…Since 2004 our laboratory has been involved in the development of cellular resolution in vivo retinal imaging systems combining adaptive optics (AO) with Fourier-domain optical coherence tomography (OCT) [1][2][3][4]. Recently we added scanning laser ophthalmoscopy (SLO) to our imaging modalities.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional cellular resolution in-vivo retinal imaging

Zawadzki

Pilli

Kim

et al. 2011

Optics in the Life Sciences

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“…However, the sample arm phase-shifting method may not be easily implemented with some existing OCT systems or more complex ones, such as those involving adaptive optics. 20 Thus, the reference arm pivot offsetting method is an interesting alternative to sample-arm-based phase shifting. The maximum phase shift achieved with our PZT was 0.45 for 1000 A-scans due to the limit of the PZT displacement.…”

Section: In Vivo Full-range Imagesmentioning

confidence: 99%

“…17 In this work we focus on evaluation of single-spectrometer based FD-OCT approaches. This is because of our laboratory experience in this type of instrumentation [18][19][20] and its greatest potential for broad implementation in existing systems. Three methods are used for phase-shifting techniques that include pivot offset scanning of the sample arm and the reference arm, as well as phase shifting with the piezoelectric actuator placed at the reference arm.…”

Section: Introductionmentioning

confidence: 99%

Comparison of phase-shifting techniques for in vivo full-range, high-speed Fourier-domain optical coherence tomography

2010

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Abstract. Single spectrometer-based complex conjugate artifact removal methods are evaluated for in vivo imaging with complementary metal-oxide semiconductor line scan camera based high-speed Fourier-domain optical coherence tomography ͑FD-OCT͒ at 100,000 axial scans per second. Performance of three different phase-shifting methods with the same OCT engine is evaluated using modified data acquisition schemes, depending on the requirements of each phaseshifting technique. The suppression ratio of complex conjugate artifact images using a paperboard is assessed for all tested methods. Several other characteristics, including a list of additional hardware requirements ͑beyond standard FD-OCT components͒ and data acquisition schemes for each of the methods is presented. In vivo full-range images of human fingerpad and nail are shown and compared with standard FD-OCT images. Additionally, a complex-conjugate-free human retinal volume acquired at the speed of 100,000 A-scans/s is presented.

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Cellular resolution volumetric in vivo retinal imaging with adaptive optics–optical coherence tomography

Cited by 78 publications

References 38 publications

Multi-MHz retinal OCT

Multi-MHz retinal OCT

Three-dimensional cellular resolution in-vivo retinal imaging

Comparison of phase-shifting techniques for in vivo full-range, high-speed Fourier-domain optical coherence tomography

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