Cellular morphologies, motility, and epithelial–mesenchymal transition of breast cancer cells incubated on viscoelastic gel substrates in hypoxia

Ishikawa, Y; Sasaki, Rie; Domura, Ryota; Okamoto, Masami

doi:10.1016/j.mtchem.2019.04.007

Cited by 9 publications

(22 citation statements)

References 51 publications

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“…Besides, substrate viscoelasticity affected motility of breast cancer cells (e.g., MDA‐MB‐231 cells) through cellular morphology (Figure 12B). [ 198 ] Cellular migration speed ( S ), cellular diffusivity ( D ), and persistent time ( P ) in MDA‐MB‐231 cells were dramatically downregulated with increase in damping coefficient (tan δ) of AAm‐ACA copolymer (AC) gels in hypoxia, independent of substrate stiffness (relative storage modulus, G ′/ρ). The tan δ values were strongly correlated to the morphological parameters including cytoplasm roundness (deformability), cellular spreading factor ( A N / A C ), and nuclear elongation factor.…”

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

“…[ 200–202 ] SNAI2 gene of MDA‐MB‐231 cells, as one of EMT transcriptional regulators, was highly expressed by the decrease in tan δ showing a robust correlation ( R 2 ≈ 0.99), suggesting the induction of EMT of MDA‐MB‐231 cells on viscoelastic substrates with low tan δ values (Figure 13B). [ 198 ] Recent studies have also linked YAP activity to EMT. YAP, a well‐known transducer of mechanical inputs from the microenvironment into biochemical responses, has been confirmed to be regulated by the substrate viscoelasticity.…”

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

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Viscoelastic Cell Microenvironment: Hydrogel‐Based Strategy for Recapitulating Dynamic ECM Mechanics

Han

Yang

et al. 2021

Adv Funct Materials

101

121

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The native extracellular matrix (ECM) generally exhibits dynamic mechanical properties and displays time‐dependent responses to deformation or mechanical loading, in terms of viscoelastic behaviors (e.g., stress relaxation and creep). Viscoelasticity of the ECM plays a critical role in development, homeostasis, and tissue regeneration, and its implication in disease progression has also been recognized recently. Hydrogels with tunable viscoelastic properties hold a great promise to recapitulate such time‐dependent mechanics found in native ECM, which have been recently used to regulate cell behavior and guide cell fate. Here the importance of tissue viscoelasticity is first highlighted, the molecular mechanisms of hydrogel viscoelasticity are summarized, and characterization techniques used at the macroscale and microscale are reviewed. Then, recent advances in developing novel hydrogels with tunable viscoelasticity through varying crosslinking strategies, engineering of viscoelastic cell microenvironment and its substantial effects on cell behavior and fate are described, and the underlying mechanobiology mechanisms are subsequently discussed. Finally, the ongoing challenges and future perspectives on the design and modulation of viscoelastic hydrogels and the mechanobiology mechanisms on cellular responses to viscoelastic cell microenvironment are proposed.

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Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

Section: Viscoelastic Cell Microenvironment Affects Cell Behavior and Fatementioning

confidence: 99%

See 2 more Smart Citations

Viscoelastic Cell Microenvironment: Hydrogel‐Based Strategy for Recapitulating Dynamic ECM Mechanics

Han

Yang

et al. 2021

Adv Funct Materials

101

121

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“…They show the great impact of stiffness on cellular behavior, but the underlying mechanisms are still not known. In turn, some other research report that besides substrate stiffness, several other material properties including the damping coefficient [ 32 ] and stress relaxation [ 33 ] of the substrates are of importance, and should be considered in the mechanotransduction mechanism [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Substrate Stiffness on Physicochemical Properties of Normal and Fibrotic Lung Fibroblasts

et al. 2020

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The presented research aims to verify whether physicochemical properties of lung fibroblasts, modified by substrate stiffness, can be used to discriminate between normal and fibrotic cells from idiopathic pulmonary fibrosis (IPF). The impact of polydimethylsiloxane (PDMS) substrate stiffness on the physicochemical properties of normal (LL24) and IPF-derived lung fibroblasts (LL97A) was examined in detail. The growth and elasticity of cells were assessed using fluorescence microscopy and atomic force microscopy working in force spectroscopy mode, respectively. The number of fibroblasts, as well as their shape and the arrangement, strongly depends on the mechanical properties of the substrate. Moreover, normal fibroblasts remain more rigid as compared to their fibrotic counterparts, which may indicate the impairments of IPF-derived fibroblasts induced by the fibrosis process. The chemical properties of normal and IPF-derived lung fibroblasts inspected using time-of-flight secondary ion mass spectrometry, and analyzed complexly with principal component analysis (PCA), show a significant difference in the distribution of cholesterol and phospholipids. Based on the observed distinctions between healthy and fibrotic cells, the mechanical properties of cells may serve as prospective diagnostic biomarkers enabling fast and reliable identification of idiopathic pulmonary fibrosis (IPF).

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Recent Innovations in Strategies for Breast Cancer Therapy by Electrospun Scaffolds: A Review

Ghaedamini,

Hashemibeni,

Honarvar

et al. 2023

J Polym Environ

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Cellular morphologies, motility, and epithelial–mesenchymal transition of breast cancer cells incubated on viscoelastic gel substrates in hypoxia

Cited by 9 publications

References 51 publications

Viscoelastic Cell Microenvironment: Hydrogel‐Based Strategy for Recapitulating Dynamic ECM Mechanics

Viscoelastic Cell Microenvironment: Hydrogel‐Based Strategy for Recapitulating Dynamic ECM Mechanics

Effect of Substrate Stiffness on Physicochemical Properties of Normal and Fibrotic Lung Fibroblasts

Recent Innovations in Strategies for Breast Cancer Therapy by Electrospun Scaffolds: A Review

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