Recent Innovations in Strategies for Breast Cancer Therapy by Electrospun Scaffolds: A Review

Today, cartilage tissue engineering (CTE) is considered important due to the lack of repair of cartilaginous lesions and the absence of appropriate methods for treatment. In this study, polycaprolactone (PCL) scaffolds were fabricated by three-dimensional (3D) printing and were then coated with fibrin (F) and acellular solubilized extracellular matrix (ECM). After extracting adipose-derived stem cells (ADSCs), 3D-printed scaffolds were characterized and compared to hydrogel groups. After inducing the chondrogenic differentiation in the presence of Piascledine and comparing it with TGF-β3 for 28 days, the expression of genes involved in chondrogenesis (AGG, COLII) and the expression of the hypertrophic gene (COLX) were examined by real-time PCR. The expression of proteins COLII and COLX was also determined by immunohistochemistry. Glycosaminoglycan was measured by toluidine blue staining. 3D-printed scaffolds clearly improved cell proliferation, viability, water absorption, and compressive strength compared to the hydrogel groups. Moreover, the use of compounds such as ECM and Piascledine in the process of ADSCs chondrogenesis induction increased cartilage-specific markers and decreased the hypertrophic marker compared to TGF-β3. In Piascledine groups, the expression of COLL II protein, COLL II and Aggrecan genes, and the amount of glycosaminoglycan showed a statistically significant increase in the PCL/F/ECM compared to the PCL and PCL/F groups (P < 0.05). The results confirmed that the PCL/F/ECM scaffolds presented in this study afford unique opportunities for CTE.

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Chitosan-incorporated Bioceramic-based Nanomaterials for Localized Release of Therapeutics and Bone Regeneration: An Overview of Recent Advances and Progresses

Safarzadeh,

Mozafari,

Naghib

2024

COC

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The usage of nanoparticles in tissue engineering applications has increased significantly in the last several years. Functional tissues are developed by regulating cell proliferation, differentiation, and migration on nanostructured scaffolds containing cells. These scaffolds provide an environment that is more structurally supportive than the microarchitecture of natural bone. Given its exceptional properties, such as its osteogenic potential, biocompatibility, and biodegradability, chitosan is a good and promising biomaterial. Unfortunately, chitosan's low mechanical strength makes it unsuitable for load-bearing applications. By mixing chitosan with other biomaterials, this drawback might be mitigated. Bone tissue engineering uses both bioresorbable materials like tricalcium phosphate and bioactive materials like hydroxyapatite and bioglass. Alumina and titanium are examples of bioinert materials that are part of these bioceramics. When produced at nanoscale scales, these materials have a larger surface area and better cell adhesion. This review paper will go into great detail on the bioinert, bioresorbable, and bioactive nanoceramics-reinforced chitosan scaffolds for bone tissue engineering.

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Recent Innovations in Strategies for Breast Cancer Therapy by Electrospun Scaffolds: A Review

Cited by 4 publications

References 138 publications

Preparation and in vitro evaluation of pH and glutathione dual-responsive drug delivery system based on sodium carboxymethyl cellulose

Preparation and in vitro evaluation of pH and glutathione dual-responsive drug delivery system based on sodium carboxymethyl cellulose

Chondrogenesis of Mesenchymal Stromal Cells on the 3D Printed Polycaprolactone/Fibrin/Decellular Cartilage Matrix Hybrid Scaffolds in the Presence of Piascledine

Chitosan-incorporated Bioceramic-based Nanomaterials for Localized Release of Therapeutics and Bone Regeneration: An Overview of Recent Advances and Progresses

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