Cellular Integration of an Enzyme‐Loaded Polymersome Nanoreactor

Dongen, Stijn F. M. van; Verdurmen, Wouter P. R.; Peters, Ruud J. R. W.; Nolte, Roeland J. M.; Brock, Roland; Hest, Jan C. M. van

doi:10.1002/anie.201002655

Cited by 137 publications

(52 citation statements)

References 34 publications

(24 reference statements)

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“…In this case, however, the self-assembly can be affected by the nature and size of the targeting units. Following this strategy, van Hest and coworkers (58) synthesized a polystyrene- block -PEG copolymer covalently attached to tat, a cell-penetrating peptide that promotes cellular uptake. The peptide-containing block copolymer was assembled together with polystyrene- block -poly[L-isocyanoalanine(2-thiophen-3-yl-ethyl)amide] (PS-PIAT), resulting in a tat-polymersome (Figure 6).…”

Section: Nanocarrier Designmentioning

confidence: 99%

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From Stealthy Polymersomes and Filomicelles to “Self” Peptide-Nanoparticles for Cancer Therapy

Oltra

Nair²,

Discher³

2014

Annu. Rev. Chem. Biomol. Eng.

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Polymersome vesicles and wormlike filomicelles self-assembled with amphiphilic, degradable block copolymers have recently shown promise in application to cancer therapy. In the case of filomicelles, dense, hydrophilic brushes of poly(ethylene glycol) on these nanoparticles combine with flexibility to nonspecifically delay clearance by phagocytes in vivo, which has motivated the development of “self” peptides that inhibit nanoparticle clearance through specific interactions. Delayed clearance, as well as robustness of polymer assemblies, opens the dosage window for delivery of increased drug loads in the polymer assemblies and increased tumor accumulation of drug(s). Antibody-targeting and combination therapies, such as with radiotherapy, are emerging in preclinical animal models of cancer. Such efforts are expected to combine with further advances in polymer composition, structure, and protein/peptide functionalization to further enhance transport through the circulation and permeation into disease sites.

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Section: Nanocarrier Designmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

From Stealthy Polymersomes and Filomicelles to “Self” Peptide-Nanoparticles for Cancer Therapy

Oltra

Nair²,

Discher³

2014

Annu. Rev. Chem. Biomol. Eng.

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“…Hence, cellular uptake of the carrier before releasing its contents may be desirable. Regarding uptake, this can be facilitated by functionalization of the carrier with cell-penetrating peptides (CPPs), which is a class of peptides capable of inducing cellular uptake of almost any kind of cargo to which they have been attached [53][54][55][56][57][58][59][60][61]. However, modification of the liposome surface with Tat-peptides leads to aspecific interaction and uptake in non-target cells [62].…”

Section: Introductionmentioning

confidence: 99%

Constrained and UV-activatable cell-penetrating peptides for intracellular delivery of liposomes

Hansen

Gaal

Minten

et al. 2012

Journal of Controlled Release

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“…35 PS 300 - b -PEG 44 -OMe and PS 280 - b -PEG 44 -Atto488 (5 wt%) block copolymers were used for the synthesis of 500 nm size polystyrene-block-polyethylene glycol polymersomes following a previously reported procedure (synthetic details can be found in the ESI). 38, 39 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of dextran(ethylene glycol) hydrogel films for giant unilamellar lipid vesicle production and their application for the encapsulation of polymersomes

et al. 2017

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Giant Unilamellar Vesicles (GUVs) prepared from phospholipids are becoming popular membrane model systems for use in biophysical studies. The quality, size and yield of GUVs depend on the preparation method used to obtain them. In this study, hydrogels consisting of dextran polymers crosslinked by poly(ethylene glycol) (DexPEG) were used as hydrophilic frameworks for the preparation of vesicle suspensions under physiological ionic strength conditions. A comparative study was conducted using hydrogels with varied physicochemical properties to evaluate their performance for GUV production. The prepared GUVs were quantified by flow cytometry using the Coulter Principle to determine yield and size distribution. We find that hydrogels of lower mechanical strength, increased swellability and decreased lipid interaction favour GUV production, while their resulting size is determined by the surface roughness of the hydrogel film. Moreover, we embedded polymersomes into the crosslinked hydrogel network, creating a DexPEG – polymersomes hybrid film. The re-hydration of lipids on those hybrid substrates led to production of GUVs and the efficient encapsulation of polymersomes in the lumen of GUVs.

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Cellular Integration of an Enzyme‐Loaded Polymersome Nanoreactor

Cited by 137 publications

References 34 publications

From Stealthy Polymersomes and Filomicelles to “Self” Peptide-Nanoparticles for Cancer Therapy

From Stealthy Polymersomes and Filomicelles to “Self” Peptide-Nanoparticles for Cancer Therapy

Constrained and UV-activatable cell-penetrating peptides for intracellular delivery of liposomes

Evaluation of dextran(ethylene glycol) hydrogel films for giant unilamellar lipid vesicle production and their application for the encapsulation of polymersomes

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