Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov

Moss, C W; Bibb, W F; Karr, Dane E.; Guerrant, G O; Lambert, M A

doi:10.1128/jcm.18.4.917-919.1983

Cited by 24 publications

(16 citation statements)

References 14 publications

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“…and Q-12 as major components in L. anisa is consistent with previous data which show that all Legionella species contain ubiquinones with more than 10-isoprene units in the side chain (15,19). DISCUSSION L. anisa strains were isolated from widely separated geographic areas of the United States and from both industrial and drinking water.…”

Section: Resultssupporting

confidence: 91%

“…Menaquinones were not detected in any strain (Table 4). This ubiquinone pattern is similar to that of L. bozemanii, L. dumoffii, and L. Iongbeachae but is distinct from that of L. pneumophila, L. micdadei, L. jordanis, L. wadsworthii, L. oakridgensis, and L. feeleii (15,19). The patterns were reproducible, as essentially identical results were obtained with each of the isolates grown and processed three separate times.…”

Section: Resultssupporting

confidence: 65%

See 1 more Smart Citation

Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower

Gorman¹,

Feeley²,

Steigerwalt³

et al. 1985

Appl Environ Microbiol

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strains of Legionella-like organisms were isolated from water. Four were recovered from potable water collected from hospitals in Chicago, Ill., and Los Angeles, Calif., during outbreaks of nosocomial legionellosis. The fifth strain was isolated from water collected from an industrial cooling tower in Jamestown, N.Y. The strains exhibited biochemical reactions typical of Legionella species and were gram-negative motile rods which grew on buffered charcoal-yeast extract agar but not on blood agar, required cysteine, and were catalase positive, urease negative, nitrate negative, hippurate negative, and nonfermentative. All strains were positive for oxidase and beta-lactamase and produced a brown, diffusible pigment. Of the five strains, four exhibited blue-white autofluorescence under long-wavelength UV light. The fatty-acid composition and ubiquinone content of these strains were consistent with those of other Legionella species. Direct fluorescent-antibody examination of the five strains with conjugates to previously described Legionella species demonstrated no cross-reactions except with the conjugates to L. longbeachae serogroup 2 and L. bozemanii serogroup 2. Four strains gave a 4+ reaction to the L. Iongbeachae serogroup 2 conjugate and the fifth strain gave a 1 + reaction. Each of the five strains gave a 4+ reaction with the conjugate to L. bozemanii serogroup 2. DNAs from the five strains were highly related (84 to 99%) and showed 5 to 57% relatedness to other Legionella species. These strains constitute a new species in the genus Legionella, and the name Legionella anisa sp. nov. is proposed. The type strain of L. anisa is WA-316-C3 (ATCC 35292).

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 65%

Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower

Gorman¹,

Feeley²,

Steigerwalt³

et al. 1985

Appl Environ Microbiol

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“…Chemical composition. The nonhydroxylated and monohydroxylated fatty acid profiles of all strains were consistent with those published previously for L. feeleii (8,10,11). No dihydroxylated fatty acids were found in any of these strains.…”

Section: Methodssupporting

confidence: 90%

Second serogroup of Legionella feeleii strains isolated from humans

Thacker¹,

Wilkinson²,

Plikaytis³

et al. 1985

J Clin Microbiol

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Three strains of Legionellafeeleii from patients with pneumonia (425-MI-H, 691-WI-H, and 693-WI-H) and one environmental strain (713-MI-E) received at the Centers for Disease Control for reference diagnostic testing were compared with the type strain WO-44C-C3 (ATCC 35072) by DNA hybridization, chemical analysis of cellular fatty acids and ubiquinones, biochemical tests, and serological characteristics. All four isolates were assigned to the L. feeleii species on the basis of DNA hybridization results. However, strains 691-WI-H and 693-WI-H were serologically distinct from strain WO-44C-C3, as shown by their minimal reactivity (1 to 2+) with a direct immunofluorescence conjugate prepared against L. feeleii serogroup 1 (strain WO-44C-C3). Therefore, strains 691-Wl-H and 693-WI-H were placed in a new L. feeleii serogroup (serogroup 2). The reference strain of L. feeleii serogroup 2 is 691-WI-H (ATCC 35849).

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“…Species J (isolate IMVS-933) has grouped with L. feeleii. While the isolate produces a ubiquinone profile similar to that of L. feeleii (27), the fatty acid profile shows some differences. The significance of this result is unclear, and the identity of this isolate also needs to be further elucidated.…”

Section: Discussionmentioning

confidence: 88%

Sequence-Based Classification Scheme for the Genus Legionella Targeting the mip Gene

et al. 1998

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The identification and speciation of strains ofLegionella is often difficult, and even the more successful chromatographic classification techniques have struggled to discriminate newly described species. A sequence-based genotypic classification scheme is reported, targeting approximately 700 nucleotide bases of the mip gene and utilizing gene amplification and direct amplicon sequencing. With the exception ofLegionella geestiana, for which an amplicon was not produced, the scheme clearly and unambiguously discriminated among the remaining 39 Legionella species and correctly grouped 26 additional serogroup and reference strains within those species. Additionally, the genotypic classification of approximately 150 wild strains from several continents was consistent with their phenotypic classification, with the exception of a few strains where serological cross-reactivity was complex, potentially confusing the latter classification. Strains thought to represent currently uncharacterized species were also found to be genotypically unique. The scheme is technically simple for a laboratory with even basic molecular capabilities and equipment, if access to a sequencing laboratory is available.

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Cellular fatty acid composition and ubiquinone content of Legionella feeleii sp. nov

Cited by 24 publications

References 14 publications

Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower

Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower

Second serogroup of Legionella feeleii strains isolated from humans

Sequence-Based Classification Scheme for the Genus Legionella Targeting the mip Gene

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