Cellular Fatty Acid Analysis of Isolates of Bacillus thuringiensis Serovar kurstaki, Strain HD-1

Siegel, Joel P.; Smith, Alistair; Novak, Robert J.

doi:10.1006/bcon.1999.0770

Cited by 5 publications

(8 citation statements)

References 17 publications

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“…These results are similar to those in literature (Sneath, 1986;Siegel et al, 2000). In addition, the serotyping analysis of this isolate showed that it is an acrystalliferous form of Bacillus thuringiensis subsp.…”

Section: Resultssupporting

confidence: 92%

The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

et al. 2006

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Abstract:The European spruce bark beetle, Dendroctonus micans Kugelann (Coleoptera, Scolytidae), is one of the most serious pests of oriental spruce (Picea orientalis L.) in Turkey. In this study, we investigated bacterial flora of D. micans collected from different populations of the forests of Eastern Black Sea Region of Turkey from 2002 to 2004. Seven different bacteria were isolated from healthy, diseased and dead specimens based on the color of colony and morphology. According to morphological, physiological and biochemical properties, metobolic enyzme profile by BIOLOG microtiter plate system, and total cellular fatty acid profile by Microbial Identification System (MIS), isolates were identified as Micrococcus luteus, Bacillus thuringiensis subsp. morrisoni, Serratia grimesii, Enterobacter cloaceae, Enterobacter intermedius, Streptococcus sp. and Pseudomonas putida. This is the first study on the bacterial flora of D. micans.

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Section: Resultssupporting

confidence: 92%

The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

et al. 2006

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“…al (1997) the presence of the entFM gene is highly likely when a complete set of products from the TY-primer sets listed in Table 2 is present b Score according to manufacturers' manual (+++ highest activity, -no activity) c The strains have been grouped according to distinct RAPD patterns This may be related to the fact that plasmids can be lost or recombined, generating new and different plasmid patterns from strains of the same origin. Additionally, based on cellular fatty acid analysis, Siegel et al (2000) have found that this commercial product, Dipel, is probably not based on a single B. thuringiensis type.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of Bacillus cereus-like bacteria from faecal samples from greenhouse workers who are using Bacillus thuringiensis-based insecticides

Jensen

Larsen

Jacobsen

et al. 2002

IAOEH

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The methods applied have verified that the faecal isolate was identical to the B. thuringiensis isolate found in the biopesticide used. This is the first reported case of isolation of a bacterial biopesticide from human faeces. The biopesticide was shown to harbour and express enterotoxin genes. However, there is no evidence that this caused any adverse effects to the person from whom these bacteria were isolated.

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“…However, the applicability of these methods for differentiation between Btk strains/variants has not been demonstrated. The best available methods include ribosomal/plasmid/nucleic acid profiling ( − ), crystal serology (), and cellular fatty acid (CFA) fingerprinting ( − ). Of these methods, CFA fingerprinting and automated ribosomal/nucleic acid profiling represent state-of-the-art technology for bacterial identification and differentiation at the species level.…”

Section: Introductionmentioning

confidence: 99%

“…This technique is used routinely for medical identification of isolates and has been used by others in the comparative characterization of various bacteria at the subspecies level ( − ). For example, this method was used by Siegel et al to characterize commercial preparations of Bti and Btk ( , ) and Bacillus sphaericus isolates () and by Esnard et al () for the differentiation of strains of B. thuringiensis and B. thuringiensis var. kurstaki .…”

Section: Introductionmentioning

confidence: 99%

Cellular Fatty-Acid Analysis of Bacillus thuringiensis Var. kurstaki Commercial Preparations

Adams

Gurr

Hogge

2005

J. Agric. Food Chem.

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Cellular fatty acid (CFA) composition of Bacillus thuringiensis var. kurstaki (Btk) preparations was determined by use of the MIDI Sherlock microbial identification system on a Hewlett-Packard 5890 gas chromatograph. Four commercial preparations--one Btk sample obtained from the U.S. Forest Service, one Btk sample obtained from Dugway Proving Ground, and Btk and Bacillus thuringiensis var. israelensis (Bti) preparations obtained from American Type Culture Collection (ATCC)--were analyzed and evaluated. This study demonstrated the capability to detect the strain variation in the bacterial species Btk and to clearly differentiate strain variants on the basis of qualitative and quantitative differences in hydrolyzable whole CFA compositions in the preparations examined. We conclude that CFA analysis may be used to identify commercial products but that a more intensive study would be required to evaluate the potential of CFA to provide an inexpensive screening tool applicable to several levels of isolate or product evaluation, including how applied preparations might interact with natural populations over time.

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Cellular Fatty Acid Analysis of Isolates of Bacillus thuringiensis Serovar kurstaki, Strain HD-1

Cited by 5 publications

References 17 publications

The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

Isolation and characterization of Bacillus cereus-like bacteria from faecal samples from greenhouse workers who are using Bacillus thuringiensis-based insecticides

Cellular Fatty-Acid Analysis of Bacillus thuringiensis Var. kurstaki Commercial Preparations

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