The first study on the bacterial flora of the european spruce bark beetle, Dendroctonus micans (Coleoptera: Scolytidae)

Abstract

Abstract:The European spruce bark beetle, Dendroctonus micans Kugelann (Coleoptera, Scolytidae), is one of the most serious pests of oriental spruce (Picea orientalis L.) in Turkey. In this study, we investigated bacterial flora of D. micans collected from different populations of the forests of Eastern Black Sea Region of Turkey from 2002 to 2004. Seven different bacteria were isolated from healthy, diseased and dead specimens based on the color of colony and morphology. According to morphological, physiological and biochemical properties, metobolic enyzme profile by BIOLOG microtiter plate system, and total cellular fatty acid profile by Microbial Identification System (MIS), isolates were identified as Micrococcus luteus, Bacillus thuringiensis subsp. morrisoni, Serratia grimesii, Enterobacter cloaceae, Enterobacter intermedius, Streptococcus sp. and Pseudomonas putida. This is the first study on the bacterial flora of D. micans.

Gut-Associated Bacteria Throughout the Life Cycle of the Bark Beetle Dendroctonus rhizophagus Thomas and Bright (Curculionidae: Scolytinae) and Their Cellulolytic Activities

“…The culturable fraction of gut microbiota of Dendroctonus rhizophagus was integrated by seven bacterial species. Similar results have been reported in the Dendroctonus frontalis, Dendroctonus valens, and Dendroctonus micans gut, where 13, 14, and 7 culturable bacteria species were recognized, respectively [35,55,59]. The scarce gut bacterial community is a common feature in all bark beetles studied, probably due mainly to the antibacterial activity of some monoterpenes present in pine resin and/or limitation of nitrogen sources [2,53].…”

“…This bacterial diversity is slightly lower than that observed in other bark beetles [35,54,59]. The gut-associated bacterial community in pupae was lower in number and diversity than in larvae and adults (Table 2).…”

Cellulolytic Bacteria Associated with the Gut of Dendroctonus armandi Larvae (Coleoptera: Curculionidae: Scolytinae)

“…have been previously isolated or detected in Dendroctonus spp. [10,24,25]. Known for the ability to produce a myriad of extracellular enzymes such as cellulases [26], Serratia spp.…”

The first investigation of the diversity of bacteria associated with Leptinotarsa decemlineata (Coleoptera: Chrysomelidae)

“…In the literature, P. aeruginosa and P. fluorescens were frequently isolated from insects (Sezen & Demirbag 1999;Sezen et al 2004Sezen et al , 2005. In previous studies, it was reported that this bacterium could infect Euproctis chrysorrhoea (Steinhaus 1963), and Dendroctonus micans (Coleoptera: Scolytidae) (Yilmaz et al 2006). A number of Pseudomonas species are important insect pathogens, i.e., P. chlororaphis (Bucher 1981), P. fluorescens (Sezen & Demirbag 1999) and P. aeruginosa (Osborn et al 2002).…”