Cellular delivery of shRNA using aptamer-conjugated PLL-alkyl-PEI nanoparticles

Askarian, Saeedeh; Abnous, Khalil; Taghavi, Sahar; Oskuee, Reza Kazemi; Ramezani, Mohammad

doi:10.1016/j.colsurfb.2015.09.023

Cited by 44 publications

(18 citation statements)

References 57 publications

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“…For example, surface coating by polymers has been shown to confer targeting ability, colloidal stability, and/or multifunctionality. 7 Various chemicals have been used to enhance cellular uptake of NPs, including poly-l-lysine (PLL), which is also applied to gene delivery [8][9][10] and cell labeling. [11][12][13][14] The addition of PLL to cultures enhances NP internalization by stem cells 15,16 and tumor cells.…”

Section: Introductionmentioning

confidence: 99%

Interaction of poly-L-lysine coating and heparan sulfate proteoglycan on magnetic nanoparticle uptake by tumor cells

Siow¹,

Chang²,

Babič³

et al. 2018

IJN

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BackgroundPoly-l-lysine (PLL) enhances nanoparticle (NP) uptake, but the molecular mechanism remains unresolved. We asked whether PLL may interact with negatively charged glycoconjugates on the cell surface and facilitate uptake of magnetic NPs (MNPs) by tumor cells.MethodsPLL-coated MNPs (PLL-MNPs) with positive and negative ζ-potential were prepared and characterized. Confocal and transmission electron microscopy was used to analyze cellular internalization of MNPs. A colorimetric iron assay was used to quantitate cell-associated MNPs (MNPcell).ResultsCoadministration of PLL and dextran-coated MNPs in culture enhanced cellular internalization of MNPs, with increased vesicle size and numbers/cell. MNPcell was increased by eight- to 12-fold in response to PLL in a concentration-dependent manner in human glioma and HeLa cells. However, the application of a magnetic field attenuated PLL-induced increase in MNPcell. PLL-coating increased MNPcell regardless of ζ-potential of PLL-MNPs, whereas magnetic force did not enhance MNPcell. In contrast, epigallocatechin gallate and magnetic force synergistically enhanced PLL-MNP uptake. In addition, heparin, but not sialic acid, greatly reduced the enhancement effects of PLL; however, removal of heparan sulfate from heparan sulfate proteoglycans of the cell surface by heparinase III significantly reduced MNPcell.ConclusionOur results suggest that PLL-heparan sulfate proteoglycan interaction may be the first step mediating PLL-MNP internalization by tumor cells. Given these results, PLL may facilitate NP interaction with tumor cells via a molecular mechanism shared by infection machinery of certain viruses.

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Section: Introductionmentioning

confidence: 99%

Interaction of poly-L-lysine coating and heparan sulfate proteoglycan on magnetic nanoparticle uptake by tumor cells

Siow¹,

Chang²,

Babič³

et al. 2018

IJN

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“…Two of them were developed aiming at the delivery of shRNA plasmid for specific knockdown of Bcl-xL protein. The first one consisted of a PLL–alkyl–PEI copolymer (as in Figure 5 a) [ 81 ] while the second used modified PAMAM with 10-bromodecanoic acid (10C) and 10C-PEG (as in Figure 5 b) [ 82 ]. Both systems were decorated with AS1411 aptamer for targeting nucleolin on target cancer cells.…”

Section: Aptamer-based Conjugates For Targeted Delivery In Nsclcmentioning

confidence: 99%

Advances in Oligonucleotide Aptamers for NSCLC Targeting

Rotoli

Santana-Viera

Ibba

et al. 2020

IJMS

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Non-small-cell lung cancer (NSCLC) is the most common type of lung cancer worldwide, with the highest incidence in developed countries. NSCLC patients often face resistance to currently available therapies, accounting for frequent relapses and poor prognosis. Indeed, despite great recent advancements in the field of NSCLC diagnosis and multimodal therapy, most patients are diagnosed at advanced metastatic stage, with a very low overall survival. Thus, the identification of new effective diagnostic and therapeutic options for NSCLC patients is a crucial challenge in oncology. A promising class of targeting molecules is represented by nucleic-acid aptamers, short single-stranded oligonucleotides that upon folding in particular three dimensional (3D) structures, serve as high affinity ligands towards disease-associated proteins. They are produced in vitro by SELEX (systematic evolution of ligands by exponential enrichment), a combinatorial chemistry procedure, representing an important tool for novel targetable biomarker discovery of both diagnostic and therapeutic interest. Aptamer-based approaches are promising options for NSCLC early diagnosis and targeted therapy and may overcome the key obstacles of currently used therapeutic modalities, such as the high toxicity and patients’ resistance. In this review, we highlight the most important applications of SELEX technology and aptamers for NSCLC handling.

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“…Ligand conjugation to nano-particles is a widely used technique that allows to extend application of functionalized nano-structures in diverse fields, such as gene or drug delivery systems (Askarian et al 2017(Askarian et al , 2015, biomedical imaging process, drug design, and vaccine development strategies (Sapsford et al 2013). Casciaro et al (2017) covalently conjugated an Esculentin-1a derivative, Esc (1-21) NH2, to gold nanoparticles (AuNPs) using poly(ethylene glycol) linkers.…”

Section: Brevinin Functionalized Nanostructurementioning

confidence: 99%

Biological Properties, Current Applications and Potential Therapeautic Applications of Brevinin Peptide Superfamily

Zohrab

Askarian

Jalili

et al. 2018

Int J Pept Res Ther

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The Brevinin peptides are antimicrobial agents obtained from frog skin secretions. Brevinin-2R has attracted many attentions due to its very low hemolytic activity, cationic property, and high affinity to cancer cells. Moreover, it has shown little toxicity against normal mammalian cells, while having killed several tumor cell lines by activation of lysosome-mitochondrial death pathway. In this review, we introduced the Brevinin superfamily with a focus on its therapeutic applications. Next, some unique properties of Brevinins were briefly discussed, including their ability to stimulate insulin secretion, dendritic cell maturation, and wound healing. In this context, we also provide information about the decoration of nanoparticles, such as cerium nano-oxide, by Brevinins. Finally, we addressed their potential for anti-tumor and drug design applications.

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Cellular delivery of shRNA using aptamer-conjugated PLL-alkyl-PEI nanoparticles

Cited by 44 publications

References 57 publications

Interaction of poly-L-lysine coating and heparan sulfate proteoglycan on magnetic nanoparticle uptake by tumor cells

Interaction of poly-L-lysine coating and heparan sulfate proteoglycan on magnetic nanoparticle uptake by tumor cells

Advances in Oligonucleotide Aptamers for NSCLC Targeting

Biological Properties, Current Applications and Potential Therapeautic Applications of Brevinin Peptide Superfamily

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