Cellular compatibility of a gamma-irradiated modified siloxane-poly(lactic acid)-calcium carbonate hybrid membrane for guided bone regeneration

Abstract

A bi-layered silicon-releasable membrane consisting of a siloxane-poly(lactic acid) (PLA)-vaterite hybrid material (Si-PVH) microfiber mesh and a PLA microfiber mesh has been developed by an electrospinning method for guided bone regeneration (GBR) application. The bi-layered membrane was modified to a three-laminar structure by sandwiching an additional PLA microfiber mesh between the Si-PVH and PLA microfiber meshes (Si-PVH/PLA membrane). In this study, the influence of gamma irradiation, used for sterilization, on biological properties of the Si-PVH/PLA membrane was evaluated with osteoblasts and fibroblasts. After gamma irradiation, while the average molecular weight of the Si-PVH/PLA membrane decreased, the Si-PVH/PLA membrane promoted cell proliferation and differentiation (alkaline phosphatase activity and calcification) of osteoblasts, compared with the poly(lactide-co-glycolide) membrane. These results suggest that the gamma-irradiated Si-PVH/PLA membrane is biocompatible with both fibroblasts and osteoblasts, and may have an application for GBR.

Safety Evaluation of a Bioglass–Polylactic Acid Composite Scaffold Seeded with Progenitor Cells in a Rat Skull Critical-Size Bone Defect

“…They observed no test material-related effects in mortality, inflammation, hematology, serum biochemistry parameters and organ weight in the operated animals at 8, 12, and 24 weeks after implantation [33]. Other scientists have analyzed the biocompatibility of PLA to cells and consistently report good adhesion and no acute cytotoxic effects on fibroblasts [49], MSCs [50], osteoblasts [51] or endothelial cells [52].…”

Osteoconductive Potential of Barrier NanoSiO2PLGA Membranes Functionalized by Plasma Enhanced Chemical Vapour Deposition

“…For biological applications, there is an additional need for versatile deposition methods in the case of devices with potential clinical applications, which should also stand, without alterations, the sterilization processes [13, 16, 18]. We have successfully demonstrated that PECVD TiO 2 functionalization methodology employed by our group enhances HOB cells response to nonresorbable PET devices and stands for standard u.v.…”

“…Well-dispersed nanosized inorganic particles have been described to enhance the mechanical properties of organic materials [10]. A recent study on biosilica influence on the OPG-RANKL ratio in SaOS-2 cells in vitro suggests both osteoinductive and osteogenic potential of biosilica since it combines an upregulation of the genes required for the differentiation with the potential to increase proliferation of the osteogenic cells [18, 24]. …”

“…Although the bulk properties of materials are important for the overall properties, especially for mechanical strength, a topographical modification of the cell-substrate interface is of utmost importance. Thus, the possibility of tailoring membrane surfaces, in particular in biodegradable devices with bioactive properties, to create modified biomaterials that mimic natural bone extracellular matrix (ECM) and stimulate osteoblast response should make them more suitable for clinical use, hopefully enhancing bone regeneration in the near future [1, 2, 18]. …”

<i>In vitro</i> evaluation of electrospun PLGA/PLLA/PDLLA blend fibers loaded with naringin for guided bone regeneration

“…The materials selected for drug loading include poly lactic acid (PLA), poly(lactide-co-glycolide) (PLGA), poly(D, L-lactic acid) (PDLLA), polycaprolactone (PCL), and other biocompatible and biodegradable polymers 6,16,19,20) . Among different classes of biodegradable polymers, the thermoplastic aliphatic poly(esters), such as PLA, PLGA and so on, have generated much interest due to their favorable properties 5,[19][20][21][22][23] .…”