Cellular calcium distribution in fetal bones studied with K-pyroantimonate

Burger, E. H.; Matthews, James L.

doi:10.1007/bf02013254

Cited by 21 publications

(5 citation statements)

References 23 publications

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“…The presence of mitochondrial inorganic granules in the cells of normal (Gonzales and Karnovsky 1961; Arsenis 1972;Holtrop 1972;Shapiro and Lee 1975;Burger and Matthews 1978) and pathologically altered Sadun 1972, 1973;Cavallero et al 1974; calcified tissues (see Sects. 4.6.1 and 5.6.1) has prompted the proposal that they may play a direct role in calcification.…”

Section: Cells and Calcification: Mitochondriamentioning

confidence: 99%

“…In the mitochondria of normal tissues, granules appear to be especially plentiful when specimens are processed by freezing methods (Ali and Wisby 1975;Landis et al 1977b;Landis and Glimcher 1982;Manston and Katchburian 1983;Takano et al 1989), are prepared anhydrously (Landis et al 1977a(Landis et al , 1980, or are treated with potassium pyroantimonate (Tandler and Kierszenbaum 1971;Schäfer 1973;Brighton and Hunt 1974Burger and Matthews 1978;Morris and Appleton 1980;Silbermann 1982, 1990;Kogaya and Furahashi 1988). In the mitochondria of normal tissues, granules appear to be especially plentiful when specimens are processed by freezing methods (Ali and Wisby 1975;Landis et al 1977b;Landis and Glimcher 1982;Manston and Katchburian 1983;Takano et al 1989), are prepared anhydrously (Landis et al 1977a(Landis et al , 1980, or are treated with potassium pyroantimonate (Tandler and Kierszenbaum 1971;Schäfer 1973;Brighton and Hunt 1974Burger and Matthews 1978;Morris and Appleton 1980;Silbermann 1982, 1990;Kogaya and Furahashi 1988).…”

Section: Cells and Calcification: Mitochondriamentioning

confidence: 99%

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Biological Calcification

2007

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Section: Cells and Calcification: Mitochondriamentioning

confidence: 99%

Section: Cells and Calcification: Mitochondriamentioning

confidence: 99%

Biological Calcification

2007

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“…In order to assist conventional microscopic imaging of the dynamic events of calcification, many techniques have been used: pyroantimonate staining for Ca (9,10), electron probe x-ray microanalysis for detection of elements (2,11), cationic dyes for staining proteoglycans (12), specific immunofluorescence to localize both monomer core protein and link proteins of proteoglycans (13), and ultracryomicrotomy for the increased preservation of matrix ground substance (2,14). These techniques have helped greatly in establishing the present state of knowledge.…”

mentioning

confidence: 99%

Quantitative spatial distributions of calcium, phosphorus, and sulfur in calcifying epiphysis by high resolution electron spectroscopic imaging.

Arsenault¹,

Ottensmeyer²

1983

Proc. Natl. Acad. Sci. U.S.A.

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Electron spectroscopic imaging, a new technique that permits the quantitative detection of the spatial distributions of atomic elements at high resolution, has been applied to the epiphyseal zone of hypertrophy in the mouse for the visualization of calcium, phosphorus, and sulfur. Longitudinally sectioned epiphyseal growth plates reveal a developmental sequence in the longitudinal septum leading from a noncalcified matrix to a calcified matrix. During the early stages of this transition, matrix granules containing highly localized concentrations of P (200-400 atoms/nm2) are found spatially separate from Ca-containing sites.These Ca localizations displayed a concentration range of 20-350 atoms/nm2 and a complete spatial overlap with sulfur. At these sites, S levels range from 10 to 200 atoms/nm2. At a later stage, and therefore more proximal to the zone of provisional calcification, the usual scattered, irregularly shaped mineral deposits are found. These sites contain a virtual superposition of Ca with both P and S. The Ca/P and Ca/S ratios of these mineral deposits are predominantly 1.0 with only minor, locally varying ratios present.A fundamcntal problem in the study of calcifying tissues is the identification and characterization of the site of initial mineralization-the nucleation site. The organic matrices of cartilage, bone, and dentin possess common structural components although organized in different characteristic fashions. From ultrastructural and biochemical studies, various components of these calcifying matrices and their parenchyma have been implicated in the role of nucleation-e.g., collagen fibers (1), matrix vesicles (2-4), mitochondria (5), y-carboxyglutamic acid (6), lipids, and Ca-phospholipid-PO4 complexes (7,8). Of these components, various ones may interact in concert to initiate nucleation or to control it in some way. However, the exact mechanism of matrix-mediated mineralization remains controversial.In order to assist conventional microscopic imaging of the dynamic events of calcification, many techniques have been used: pyroantimonate staining for Ca (9, 10), electron probe x-ray microanalysis for detection of elements (2, 11), cationic dyes for staining proteoglycans (12), specific immunofluorescence to localize both monomer core protein and link proteins of proteoglycans (13), and ultracryomicrotomy for the increased preservation of matrix ground substance (2,14). These techniques have helped greatly in establishing the present state of knowledge. However, the techniques have limited the definition of a nucleation site to a Ca-containing mineral deposit with thousands of Ca atoms.In the work reported here, a new technique known as electron spectroscopic imaging was used to study the quantitative distribution of Ca, P, and S in the epiphyseal growth plate. This technique enables the direct visualization of elements in entire images with a spatial resolution as fine as 0.3-0.5 nm and with a minimum detection level as low as 2 X 10-21 g (or 50 atoms for P) (15, 16). Compared ...

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“…The presence of mitochondrial inorganic granules in the cells of normal (Gonzales and Karnovsky 1961;Martin and Matthews 1969;Arsenis 1972;Holtrop 1972;Shapiro and Lee 1975;Burger and Matthews 1978) and pathologically altered Sadun 1972, 1973;Brandt and Bässler 1972;Cavallero et al 1974;Boivin 1975) calcified tissues (see Sects. 4.6.1 and 5.6.1) has prompted the proposal that they may play a direct role in calcification.…”

Section: Cells and Calcification: Mitochondriamentioning

confidence: 99%

“…It is universally accepted that mitochondria contribute to the transport of intracellular calcium, but serious doubts exist about their direct participation in the extracellular calcification process. In the mitochondria of normal tissues, granules appear to be especially plentiful when specimens are processed by freezing methods (Ali and Wisby 1975;Landis et al 1977b;Ali et al 1978;Landis and Glimcher 1982;Manston and Katchburian 1983;Takano et al 1989), are prepared anhydrously (Landis et al 1977a(Landis et al , 1980, or are treated with potassium pyroantimonate (Tandler and Kierszenbaum 1971;Schäfer 1973;Brighton and Hunt 1974, 1976, 1978Oberc and Engel 1977;Burger and Matthews 1978;Morris and Appleton 1980;Silbermann 1982, 1990;Kogaya and Furahashi 1988). Regrettably, these methods of study, which apparently offer the best way of preserving calcium ions in cells, may cause changes in mitochondria that facilitate their calcium accumulation.…”

Section: Cells and Calcification: Mitochondriamentioning

confidence: 99%

Main Suggested Calcification Mechanisms: Cells

Biological Calcification

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Cellular calcium distribution in fetal bones studied with K-pyroantimonate

Cited by 21 publications

References 23 publications

Biological Calcification

Biological Calcification

Quantitative spatial distributions of calcium, phosphorus, and sulfur in calcifying epiphysis by high resolution electron spectroscopic imaging.

Main Suggested Calcification Mechanisms: Cells

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