Cellular behaviours of bone marrow‐derived mesenchymal stem cells towards pristine graphene oxide nanosheets

Wei, Changbo; Liu, Zifeng; Jiang, Fangfang; Zeng, Binghui; Huang, Manna; Yu, Dongsheng

doi:10.1111/cpr.12367

Cited by 67 publications

(61 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2,3 However, the majority of the work only focuses on the biological effects of nanomaterials while their intrinsic immunomodulatory effects are often neglected. [4][5][6] Take the bone implant materials: for example, titanium (Ti) metal is extensively used in clinical practice due to its outstanding osseointegration ability. Recently, the influence of Ti implant on macrophage adhesion and the effects of macrophage activation on osseointegration have been noticed.…”

Section: Introductionmentioning

confidence: 99%

Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

Wang¹,

Meng²,

Song³

et al. 2018

IJN

View full text Add to dashboard Cite

IntroductionFabricating nanostructured surface topography represents the mainstream approach to induce osteogenesis for the next-generation bone implant. In the past, the bone implant was designed to minimize host repulsive reactions in order to acquire biocompatibility. However, increasing reports indicate that the absence of an appropriate immune response cannot acquire adequate osseointegration after implantation in vivo.Materials and methodsWe prepared different topographies on the surface of titanium (Ti) specimens by grinding, etching and anodizing, and they were marked as polished specimen (P), specimen with nanotubes (NTs) in small diameters (NT-30) and specimen with NTs in large diameters (NT-100). We evaluated the ability of different topographies of the specimen to induce osteogenic differentiation of mice bone marrow mesenchymal stem cells (BMSCs) in vitro and to induce osseointegration in vivo. Furthermore, we investigated the effect of different topographies on the polarization and secretion of macrophages, and the effect of macrophage polarization on topography-induced osteogenic differentiation of mice BMSCs. Finally, we verified the effect of macrophage polarization on topography-induced osseointegration in vivo by using Cre*RBP-Jfl/fl mice in which classically activated macrophage was restrained.ResultsThe osteogenic differentiation of mice BMSCs induced by specimen with different topographies was NT-100>NT-30>P, while the osseointegration induced by specimen with different topographies in vivo was NT-30>NT-100>P. In addition, specimen of NT-30 could induce more macrophages to M2 polarization, while specimen of P and NT-100 could induce more macrophages to M1 polarization. When co-culture mice BMSCs and macrophages on specimen with different topographies, the osteogenic differentiation of mice BMSCs was NT-30>NT-100≥P. The osseointegration induced by NT-100 in Cre*RBP-Jfl/fl mice was much better than that of wild type mice.ConclusionIt is suggested that the intrinsic immunomodulatory effects of nanomaterials are not only crucial to evaluate the in vivo biocompatibility but also required to determine the final osseointegration. To clarify the immune response and osseointegration may be beneficial for the designation and optimization of the bone implant.

show abstract

Section: Introductionmentioning

confidence: 99%

Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

Wang¹,

Meng²,

Song³

et al. 2018

IJN

View full text Add to dashboard Cite

show abstract

“…The most dominant cell types used in the included articles were mesenchymal stem cells (MSCs), including human adipose-derived stem cells (hADSCs), [17][18][19][20][21][22] Goat ADSCs, 23 human dental pulp stem cells (hDPSCs), 24 human alveolar bone marrow stem cells (hABMSCs), 25 human periodontal ligament stem cells (hPDLSCs), 26,27 and bone MSCs (BMSCs), [28][29][30][31] such as human BMSCs 19, 32-41 (hBMSCs), murine BMSCs (mBMSCs), 4, 42-49 rabbit BMSCs (rBMSCs), 50 murine MSCs cell line C3H10T1/2 51,52 and Caprine BMSCs (cMBSCs). 53 The type of the MSCs in fifteen studies 19,32,36,40,[54][55][56][57][58][59][60][61][62][63][64] was not defined.…”

Section: Cell Sourcesmentioning

confidence: 99%

“…CharacterizationSynthesized graphene, graphene derivatives and their compositions were chemically characterized using Fourier-transform infrared (FTIR)14, 21, 30, 36, 37, 42, 43, 45, 48, 61, 63, 65, 66, 68, 73 74, 75, 84-86, 91, 92 and raman spectroscopy.13, 17-19, 21, 22, 24-28 32-34, 36, 37, 40-42, 45, 47, 48, 54, 55, 57, 59, 61-65, 68, 69, 71, 72, 75-78, 86-89, 93 Also, morphology and topography of the samples were characterized by SEM,4, 13, 20-23, 25, 26, 29, 33-36, 38, 43, 45-56, 58, 60, 61, 63, 65, 66, 70, 71 73-85, 87, 91, 92 field emission scanning electron microscope (FESEM),17,40,42,44,72,79,86,90,91 transmission electron microscopy (TEM),4,18,30,31,43,44,50,53,61,64,66,71,77,80,82,86,87,[89][90][91][92][93][94] and atomic force microscope (AFM).14, 17-19, 21, 24-26, 36-40, 42, 45, 47, 49, 53, 54, 59, 62 69, 70, 77, 79, 80, 82, 89 Surface hydrophobicity of the composites and their chemical environments were evaluated by contact angle (CA) measurements14, 20, 28, 36-38, 41, 42, 46, , 70, 78, 81, 86, 88, 91, 92 were employed in the various studies to evaluate mechanical strength. Inductively coupled plasma atomic emission spectrometry (ICP-AES)…”

mentioning

confidence: 99%

In vitro effect of graphene structures as an osteoinductive factor in bone tissue engineering: A systematic review

Mohammadrezaei

Golzar

Rad

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Graphene and its derivatives have been well-known as influential factors in differentiating stem/progenitor cells toward the osteoblastic lineage. However, there have been many controversies in the literature regarding the parameters effect on bone regeneration, including graphene concentration, size, type, dimension, hydrophilicity, functionalization, and composition. This study attempts to produce a comprehensive review regarding the given parameters and their effects on stimulating cell behaviors such as proliferation, viability, attachment and osteogenic differentiation. In this study, a systematic search of MEDLINE database was conducted for in vitro studies on the use of graphene and its derivatives for bone tissue engineering from January 2000 to February 2018, organized according to the PRISMA statement. According to reviewed articles, different graphene derivative, including graphene, graphene oxide (GO) and reduced graphene oxide (RGO) with mass ratio ≤1.5 wt % for all and concentration up to 50 μg/mL for graphene and GO, and 60 μg/mL for RGO, are considered to be safe for most cell types. However, these concentrations highly depend on the types of cells. It was discovered that graphene with lateral size less than 5 µm, along with GO and RGO with lateral dimension less than 1 µm decrease cell viability. In addition, the three-dimensional structure of graphene can promote cell-cell interaction, migration and proliferation. When graphene and its derivatives are incorporated with metals, polymers, and minerals, they frequently show promoted mechanical properties and bioactivity. Last, graphene and its derivatives have been found to increase the surface roughness and porosity, which can highly enhance cell adhesion and differentiation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2284-2343, 2018.

show abstract

“…Rosa V et al showed that DPSCs were anchored to the rough surface provided by GO, as assessed by scanning electron microscopy (SEM) [26]. Moreover, GO at the concentration of 0.1 lg/ml allowed for BMSC attachment and promoted proliferation, whilst GO at 10 lg/ml was cytotoxic [27]. In a review by Mohammadrezaei D et al, up to 50 lg/ml for GO seems to be safest for most cell lines [25].…”

Section: Introductionmentioning

confidence: 99%

“…In a review by Mohammadrezaei D et al, up to 50 lg/ml for GO seems to be safest for most cell lines [25]. In addition, GO could induce BMSC and DPSC osteogenic differentiation in vitro [26,27]. GO-coated b-tricalcium phosphate scaffold increased the rate of new bone formation in vivo [28].…”

Section: Introductionmentioning

confidence: 99%

Effects of graphene oxide and graphene oxide quantum dots on the osteogenic differentiation of stem cells from human exfoliated deciduous teeth

Yang

Zhao

Chen

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

View full text Add to dashboard Cite

Graphene and its derivatives, graphene oxide (GO) and graphene oxide quantum dots (GOQDs), have recently attracted much attention as bioactive factors in differentiating stem cells towards osteoblastic lineage. The stem cells from human exfoliated deciduous teeth (SHEDs) possess the properties of selfrenewal, extensive proliferation, and multiple differentiation potential, and have gradually become one of the most promising mesenchymal stem cells (MSCs) in bone tissue engineering. The purpose of this study was to explore the effects of GO and GOQDs on the osteogenic differentiation of SHEDs. In this study, GO and GOQDs facilitated SHED proliferation up to 7 days in vitro at the concentration of 1 lg/ml. Because of their excellent fluorescent properties, GOQD uptake by SHEDs was confirmed and distributed in the SHED cytoplasm. Calcium nodules formation, alkaline phosphatase (ALP) activity, and RNA and protein expression increased significantly in SHEDs treated with osteogenic induction medium containing GOQDs but decreased with osteogenic induction medium containing GO. Interestingly, the Wnt/b-catenin signaling pathway appeared to be involved in osteogenic differentiation of SHEDs induced with GOQDs. In summary, GO and GOQDs at the concentration of 1 lg/ml promoted SHED proliferation. GOQDs induced the osteogenic differentiation of SHEDs, whilst GO slightly inhibited it.

show abstract

Cellular behaviours of bone marrow‐derived mesenchymal stem cells towards pristine graphene oxide nanosheets

Abstract: Pristine GO nanosheets at a concentration of 0.1 μg/mL provide benefits to promote BMSCs proliferation and osteogenesis under a sequential-seeding method, contributing to the use of GO for dental implantation.

Cited by 67 publications

References 46 publications

Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

Nanostructured titanium regulates osseointegration via influencing macrophage polarization in the osteogenic environment

In vitro effect of graphene structures as an osteoinductive factor in bone tissue engineering: A systematic review

Effects of graphene oxide and graphene oxide quantum dots on the osteogenic differentiation of stem cells from human exfoliated deciduous teeth

Contact Info

Product

Resources

About