Cellular Behavior Change of Macrophage After Exposure to Nanoparticles

Hsiao, Jen-Hung; Weng, Tsui-Wei; Tai, M. F.; Chen, Ya-Fang; Wang, Y H; Yang, Chuanlei; Wang, J L; Liu, Hon‐Man

doi:10.1166/jnn.2009.c163

Cited by 6 publications

(4 citation statements)

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“…Since TNF-α mediates the production of NO and neutrophil attractant chemokines such as KC/CXCL-1 and IL-8, the decrease in NO and these chemokines levels from CdTe-QD pre-exposures might actually be a consequence of reduced TNF-α levels. The suppression of TNF-α production was also reported in a study by Hsiao and colleagues (2009), who showed that macrophage exposure to magnetic NPs caused a decrease in TNF-α and IL-1β, and also a decrease in NO levels, even in response to LPS stimulation. The suppression of neutrophil chemo-attractants together with the decrease in NO and TNF-α in test cells in our study suggest that CdTe-QDs could impair the ability of these immune cells to perform their anti-microbial functions in fighting bacterial infection.…”

Section: Discussionsupporting

confidence: 74%

Cadmium telluride quantum dot nanoparticle cytotoxicity and effects on model immune responses toPseudomonas aeruginosa

2012

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This study examines dose effects of cadmium telluride quantum dots (CdTe-QDs) from two commercial sources on model macrophages (J774A.1) and colonic epithelial cells (HT29). Effects on cellular immune signalling responses were measured following sequential exposure to QDs and Pseudomonas aeruginosa strain PA01. At CdTe-QD concentrations between 10-2 and 10 µg/ml, cells exhibited changes in metabolism and morphology. Confocal imaging revealed QD internalisation and changes in cell–cell contacts, shapes and internal organisations. QD doses below 10-2 µg/ml caused no observed effects. When QD exposures at 10-7 to 10-3 µg/ml preceded PA01 (107 bacteria/ml) challenges, there were elevated cytotoxicity (5–22%, p < 0.05) and reduced levels (two- to fivefold, p < 0.001) of nitric oxide (NO), TNF-α, KC/CXC−1 and IL-8, compared with PA01 exposures alone. These results demonstrate that exposures to sub-toxic levels of CdTe-QDs can depress cell immune-defence functions, which if occurred in vivo would likely interfere with normal neutrophil recruitment for defence against bacteria.

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Section: Discussionsupporting

confidence: 74%

Cadmium telluride quantum dot nanoparticle cytotoxicity and effects on model immune responses toPseudomonas aeruginosa

2012

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“…Therefore, in the present study, it is reasonable to consider that inflammatory response evoked from the host reaction against foreign bodies, MGT, induce formation of inflammation-related DNA adducts, such as εdC and HεdC, which, being involved in C to T transitions, are more likely to contribute to genotoxicity observed in the lungs of MGT-exposed mice. Recently, several reports show that the mechanisms of (geno)toxicity induced by nanoparticles are suggested to be involved in macrophage stimulation [ 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 ]. Innate immune activation through Nalp3 inflammasomes has been suggested to play an important role in the pulmonary inflammation and fibrotic disorders of silicosis and asbestosis [ 31 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

Comprehensive DNA Adduct Analysis Reveals Pulmonary Inflammatory Response Contributes to Genotoxic Action of Magnetite Nanoparticles

Ishino

Kato

Kato³

et al. 2015

IJMS

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Nanosized-magnetite (MGT) is widely utilized in medicinal and industrial fields; however, its toxicological properties are not well documented. In our previous report, MGT showed genotoxicity in both in vitro and in vivo assay systems, and it was suggested that inflammatory responses exist behind the genotoxicity. To further clarify mechanisms underlying the genotoxicity, a comprehensive DNA adduct (DNA adductome) analysis was conducted using DNA samples derived from the lungs of mice exposed to MGT. In total, 30 and 42 types of DNA adducts were detected in the vehicle control and MGT-treated groups, respectively. Principal component analysis (PCA) against a subset of DNA adducts was applied and several adducts, which are deduced to be formed by inflammation or oxidative stress, as the case of etheno-deoxycytidine (εdC), revealed higher contributions to MGT exposure. By quantitative-LC-MS/MS analysis, εdC levels were significantly higher in MGT-treated mice than those of the vehicle control. Taken together with our previous data, it is suggested that inflammatory responses might be involved in the genotoxicity induced by MGT in the lungs of mice.

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“…This demonstrates that, at relatively low concentrations, C 60 exposure may suppress an inflammatory reaction of the coelomocytes. Other studies have also demonstrated decreases in phagocytic activity in combination with a reduction in cytokine release, after exposure to magnetic and cadmium quantum dot (CdTe-QD) nanoparticles (Hsiao et al 2009;Nguyen et al 2012). Moreover, Nguyen et al (2012) demonstrated that CdTe-QD nanoparticles, by lowering cytokine levels, could make the cells more sensitive to a bacterial infection.…”

Section: Discussionmentioning

confidence: 98%

In vitronanoparticle toxicity to rat alveolar cells and coelomocytes from the earthwormLumbricus rubellus

et al. 2012

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Sensitivity of immune cells (coelomocytes) of Lumbricus rubellus earthworms was investigated for exposure to selected nanoparticles, in order to obtain further insight in mechanisms of effects observed after in vivo C60 exposure. In the in vivo study, tissue damage appeared to occur without accompanying increased immune responses. Coelomocytes exposed in vitro to C60 showed no decrease of their cellular viability, but demonstrated a decrease in gene expression of the cytokine-like protein CCF-1, indicating immunosuppression. Experiments with NR8383 rat macrophage cells and tri-block copolymer nanoparticles were used to compare sensitivity and to demonstrate the usefulness of coelomocytes as a test system for nano-immunotoxicity, respectively. Overall, the results imply that sensitivity towards nanoparticles differs between cell types and nanoparticles. Moreover, this study indicates that injuries in absence of an immune response, observed after in vivo C60 exposure in our earlier work, are caused by immunosuppression rather than coelomocyte mortality.

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Cellular Behavior Change of Macrophage After Exposure to Nanoparticles

Cited by 6 publications

References 0 publications

Cadmium telluride quantum dot nanoparticle cytotoxicity and effects on model immune responses toPseudomonas aeruginosa

Cadmium telluride quantum dot nanoparticle cytotoxicity and effects on model immune responses toPseudomonas aeruginosa

Comprehensive DNA Adduct Analysis Reveals Pulmonary Inflammatory Response Contributes to Genotoxic Action of Magnetite Nanoparticles

In vitronanoparticle toxicity to rat alveolar cells and coelomocytes from the earthwormLumbricus rubellus

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