Cellular and transcriptomic response to treatment with the probiotic candidate Vibrio lentus in gnotobiotic sea bass (Dicentrarchus labrax) larvae

Schaeck, Marlien; Reyes-López, Felipe E.; Vallejos‐Vidal, Eva; Cleemput, Jolien Van; Duchateau, Luc; Broeck, Wim Van Den; Tort, L.; Decostere, Annemie

doi:10.1016/j.fsi.2017.01.028

Cited by 26 publications

(15 citation statements)

References 54 publications

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“…The application of probiotics in this study signi cantly improved blood and serum analytical parameters of Nile tilapia. This result is consistent with many previous studies, such as in O. niloticus(Sutthi et al, 2018, Wang et al, 2008b, Cyprinus carpio(Gupta et al, 2016), Pangasius hypophthalmus(Gobi et al, 2016), and Dicentrarchus labrax(Schaeck et al, 2017). Hemoglobin levels and numbers of red blood cells (RBC) increased in Southern cat sh, Silurus meriaionalis treated with probiotic (Wu et al, 2004) Sutthi et al (2018).…”

supporting

confidence: 90%

The effect of Pediococcus acidilactici probiotics supplementation in the feed of Nile Tilapia (Oreochromis niloticus) on water quality, growth performance, Hematological parameters, antioxidants, immunological responses, enzymatic Activity, and histology.

Eissa

Alaryani

Khattab

et al. 2022

Preprint

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The effect of adding lactic acid bacteria (Pediococcus acidilactici) to the diet on water quality, feed utilization, growth, body composition, Hematological parameters, intestinal and liver histology of Nile tilapia, haemato-biochemical parameters, antioxidants, immunological responses, and Enzymatic Activity was investigated. Fish was distributed into Four groups; the control group (T0), T1 group (2.0 g), T2 group (2.5 g), and T3 group (3.0 g) of Pediococcus acidilactici per kg of diet. 25% of Water was changed daily and feed with 31% protein was added three times daily for 56 days. In probiotics-treated groups, growth performance, feed utilization, and fish biomass increased significantly compared to control. The biochemical and immunity parameters (Amylase, Lipase, and Protease activity) and villi height were significantly improved in probiotics groups. In conclusion, adding probiotics to fish diet improved all evaluated parameters

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supporting

confidence: 90%

The effect of Pediococcus acidilactici probiotics supplementation in the feed of Nile Tilapia (Oreochromis niloticus) on water quality, growth performance, Hematological parameters, antioxidants, immunological responses, enzymatic Activity, and histology.

Eissa

Alaryani

Khattab

et al. 2022

Preprint

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“…This larval rearing system avoids the intrinsic dynamics of the conventional environment in terms of microbial community interactions ( 43 ). Subsequently, the use of gnotobiotic conditions provides information about the specific and direct interaction between an external bacterial stimulus and the host ( 50 ). Thus, the gnotobiotic culture system is an important alternative to solve the problems of a reproducible experimental setup by generating high inter-individual and inter-batch variability in the composition of the standing microbial community ( 51 ).…”

Section: Discussionmentioning

confidence: 99%

“…The expression of genes related to the adaptive immune response has been reported from few days post-hatching onward ( 8 , 50 , 56 , 57 ). At cellular level, it is accepted that T cells are the first lymphoid-cell type to appear during fish ontogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…This mechanism allows the bacteria to establish the infection and even can cause fish death as a consequence of septicemia ( 77 ). In the last years, increasing attention has been directed to exploit the bacterial weaknesses by modifying iron bioavailability to take advantage of the host iron-uptake system as a control method for bacterial infections ( 50 ). In our study, the delayed modulation of transferrin and hepcidin suggests that the iron-uptake regulation system is crucial for a successful infective process resulting in high mortalities in sea bass larvae.…”

Section: Discussionmentioning

confidence: 99%

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Modulation of Innate Immune-Related Genes and Glucocorticoid Synthesis in Gnotobiotic Full-Sibling European Sea Bass (Dicentrarchus labrax) Larvae Challenged With Vibrio anguillarum

et al. 2018

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Although several efforts have been made to describe the immunoendocrine interaction in fish, there are no studies to date focusing on the characterization of the immune response and glucocorticoid synthesis using the host–pathogen interaction on larval stage as an early developmental stage model of study. Therefore, the aim of this study was to evaluate the glucocorticoid synthesis and the modulation of stress- and innate immune-related genes in European sea bass (Dicentrarchus labrax) larvae challenged with Vibrio anguillarum. For this purpose, we challenged by bath full-sibling gnotobiotic sea bass larvae with 107 CFU mL−1 of V. anguillarum strain HI 610 on day 5 post-hatching (dph). The mortality was monitored up to the end of the experiment [120 hours post-challenge (hpc)]. While no variations were registered in non-challenged larvae maintained under gnotobiotic conditions (93.20% survival at 120 hpc), in the challenged group a constant and sustained mortality was observed from 36 hpc onward, dropping to 18.31% survival at 120 hpc. Glucocorticoid quantification and expression analysis of stress- and innate immunity-related genes were carried out in single larvae. The increase of cortisol, cortisone and 20β-dihydrocortisone was observed at 120 hpc, although did not influence upon the modulation of stress-related genes (glucocorticoid receptor 1 [gr1], gr2, and heat shock protein 70 [hsp70]). On the other hand, the expression of lysozyme, transferrin, and il-10 differentially increased at 120 hpc together with a marked upregulation of the pro-inflammatory cytokines (il-1β and il-8) and hepcidin, suggesting a late activation of defense mechanisms against V. anguillarum. Importantly, this response coincided with the lowest survival observed in challenged groups. Therefore, the increase in markers associated with glucocorticoid synthesis together with the upregulation of genes associated with the anti-inflammatory response suggests that in larvae infected with V. anguillarum a pro-inflammatory response at systemic level takes place, which then leads to the participation of other physiological mechanisms at systemic level to counteract the effect and the consequences of such response. However, this late systemic response could be related to the previous high mortality observed in sea bass larvae challenged with V. anguillarum.

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“…Significantly modified gene expression (i.e., immune response, cell proliferation and death, cell adhesion, ROS metabolism and iron transport (p < 0.05). No significant differences in apoptotic and cell proliferative indexes [47] CFU: colony forming units, DWG: daily weight gain, MPO: myloperoxidase, h: hour, defb: beta defensin, RBA: respiratory burst activity, GH: growth hormone, IGF: insulin-like growth factor, dph: days post hatching, ROS: reactive oxygen species, ACH 50 : serum natural complement hemolytic activity, GST: Glutathione-S-transferase, GR: reduced glutathione.…”

Section: Benefits Of Probiotic Administration Through Watermentioning

confidence: 99%

Administration of Probiotics in the Water in Finfish Aquaculture Systems: A Review

Jahangiri

Esteban

2018

Fishes

124

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Over the last few decades, the contribution of aquaculture to animal protein production has increased enormously, and the sector now provides almost half of the fish and shellfish consumed worldwide, making it a major food producer. Nevertheless, many factors, including infections, pollution, and stress, may result in significant economic losses. The aquaculture industry will not be totally successful without the therapeutic and preventive means to control all these factors. Antibiotics (long used in aquaculture practice) have tended to aggravate the problem by increasing antibiotic resistance. Concomitantly, probiotics have widely been suggested as eco-friendly alternatives to antibiotics. However, the way in which probiotics are applied in aquaculture is a key factor in their favorable performance. The aim of this review was to examine the current state of probiotics administration through the water in finfish aquaculture. The review also attempts to cover the research gaps existing in our knowledge of this administration mode, and to suggest the issues that need to be investigated in greater depth.

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Cellular and transcriptomic response to treatment with the probiotic candidate Vibrio lentus in gnotobiotic sea bass (Dicentrarchus labrax) larvae

Cited by 26 publications

References 54 publications

The effect of Pediococcus acidilactici probiotics supplementation in the feed of Nile Tilapia (Oreochromis niloticus) on water quality, growth performance, Hematological parameters, antioxidants, immunological responses, enzymatic Activity, and histology.

The effect of Pediococcus acidilactici probiotics supplementation in the feed of Nile Tilapia (Oreochromis niloticus) on water quality, growth performance, Hematological parameters, antioxidants, immunological responses, enzymatic Activity, and histology.

Modulation of Innate Immune-Related Genes and Glucocorticoid Synthesis in Gnotobiotic Full-Sibling European Sea Bass (Dicentrarchus labrax) Larvae Challenged With Vibrio anguillarum

Administration of Probiotics in the Water in Finfish Aquaculture Systems: A Review

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