Cellular and subcellular distribution of laminin in adult rat anterior pituitary.

Vila-Porcile, Évelyne; Picart, R; Tixier‐Vidal, A.; Tougard, Claude

doi:10.1177/35.3.3029213

Cited by 59 publications

(44 citation statements)

References 29 publications

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“…In normal adenohypophysis parenchyma laminin is abundantly expressed [46]. Laminin was observed to be expressed in basement membranes and in endocrine cells and nonendocrine folliculo-stellate cells of the rat pituitary [38,40,47] in vivo and in primary cultures. The presence of laminin in the adenohypophysis of lactating female rats was also demonstrated [48].…”

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 97%

“…Similar distribution was observed in normal human pituitaries. In the pituitary gland of the adult rat laminin immunoreactivity has been found intracellularly within membranous cisternae of endocrine cells of the anterior lobe [40,47]. Labelled secretory granules were observed in gonadotrophs, thyrotrophs, corticotrophs, and lactotrophs; however, not usually in somatotrophs: each glandular cell type seemed to display a specific pattern of the subcellular distribution of laminin immunoreactivity.…”

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 97%

“…Laminin has been detected intracellularly in lactotrophs [40,47,48]. In the normal pituitary, cells expressing PRL are sited close to laminin-containing cells [46].…”

Section: Lactotrophsmentioning

confidence: 99%

“…They influenced the organisation of the endocrine cells and their secretory activity. Basement membranes of endothelial and perivascular connective tissue cells express many ECMassociated components, such as different isoforms of laminin and fibronectin, collagen-IV, entactin, and heparan sulphate proteoglycans, associated with the corresponding integrin subunits on cell membranes [40][41][42][43][44][45]. The presence of the traditional molecules that are involved in ECM activities were reported in the pituitary almost three decades ago.…”

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 99%

“…Labelled secretory granules were observed in gonadotrophs, thyrotrophs, corticotrophs, and lactotrophs; however, not usually in somatotrophs: each glandular cell type seemed to display a specific pattern of the subcellular distribution of laminin immunoreactivity. It was suggested that the intracellular distribution of laminin reflected either a vesicular pathway for laminin export or an exocytotic route by which membrane-bound laminin can be exposed on the cell surface [40].…”

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 99%

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Extracellular Matrix Proteins in the Anterior Pituitary Gland

Kanasaki¹

2011

TONEUROEJ

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Abstract; The importance of the extracellular matrix (ECM) has become recognised as an important component of the functioning of cells. However the roles of the ECM in the anterior pituitary gland have received little attention. The pituitary has the particular characteristics of possessing a number of endocrine cells as well as less well studied nonendocrine cells. Because each cell has specific and vital roles in the physiology being of an individual the ECM must also possess selective activities. The details of ECM behaviour have not yet been delineated in the pituitary and this review first considers general characteristics of ECM compounds and then examines the properties of peptides with regard to functioning of the cells of the pituitary. It is noted that there are variations between ECM associated with normal cells and with tumours, that there are both morphological and biochemical responses to alterations in the composition of substrate on which cells are grown, and that a development of further models of ECM will be required for continued advances in the understanding of the manner in which the pituitary behaves in normal, clinical and pathological circumstances. Overall, the observations suggest that modulation of the ECM provides opportunities for enhancing well-being of individuals.

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Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 97%

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 97%

“…Laminin has been detected intracellularly in lactotrophs [40,47,48]. In the normal pituitary, cells expressing PRL are sited close to laminin-containing cells [46].…”

Section: Lactotrophsmentioning

confidence: 99%

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 99%

Section: Ecm and The Anterior Pituitary Glandmentioning

confidence: 99%

See 3 more Smart Citations

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Kanasaki¹

2011

TONEUROEJ

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Distribution of laminin in the murine pituitary

et al. 1990

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The distribution and cellular localization of the glycoprotein laminin were investigated by light and electron microscopic immunocytochemistry in the adult murine pituitary gland. Immunoblots confirmed that laminin was the only protein in the pituitary gland of the adult male mouse to react with antilaminin serum. Laminin immunoreactivity was demonstrated at the light microscopic level simultaneously with that of beta-follicle stimulating hormone (beta-FSH) and beta-luteinizing hormone (beta-LH). In addition to its distribution is basal laminae, laminin immunoreactivity was coincidently expressed in gonadotrophs with the immunoreactivities of beta-FSH and beta-LH. Electron microscopic immunocytochemistry was employed on aldehyde-fixed sections embedded in L.R. White. Sites of binding of primary antisera to laminin were identified with affinity-purified secondary antisera directly coupled to 20 nm particles of colloidal gold. Three antisera recognizing laminin were compared and found to result in an identical pattern of immunoreactivity. Laminin was found extracellularly only in formed basal laminae in all three lobes of the pituitary and was not found in extracellular matrices of connective tissue. Laminin immunoreactivity was also found intracellularly in gonadotrophs but in none of the other endocrine or non-endocrine cells of the anterior lobe. Within gonadotrophs, only secretory granules were labeled. The majority, but not all, secretory granules were labeled in each of the gonadotrophs examined, and the proportion of granules labeled with laminin could not be increased by doubling the concentration of anti-laminin serum. Laminin immunoreactivity segregated with the subset of secretory granules containing beta-FSH. In contrast, laminin immunoreactivity was absent in the smaller subset of secretory granules that contain serotonin.(ABSTRACT TRUNCATED AT 250 WORDS)

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Immunolocalization of laminin, heparan‐sulfate proteoglycan, entactin, and type IV collagen in the rat anterior pituitary. I. An in vivo study

et al. 1992

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The distribution of three components of basement membranes (BM): heparan-sulfate proteoglycan (HSPG), entactin (ENT), and type IV collagen (Coll. IV), was studied in the adult rat anterior pituitary and compared to the distribution of laminin (LAM) described in an earlier report (Vila-Porcile et al., 1987, J. Histochem. Cytochem., 35, 287). Several immunocytochemical methods were applied at both light and electron microscope levels. The three components were detected in all the pituitary BM and in endothelial and perivascular connective cells, as previously observed for LAM. In contrast to the prominent labeling previously observed for LAM within epithelial cells, however, only a faint immunoreaction could be detected for the other components, with nonetheless a discrete signal for Coll. IV. These findings indicate that 1) the four studied components are present in all the BM of the rat anterior pituitary; 2) pituitary endocrine cells contain LAM, and to a lesser extent Coll. IV, and thus could participate to the elaboration of the BM; and 3) the presence of the four components in non-epithelial cells also suggests their cooperation in BM building. The questions of the turnover and intracellular pathways of each of these components were addressed but remain unanswered.

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Cellular and subcellular distribution of laminin in adult rat anterior pituitary.

Cited by 59 publications

References 29 publications

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Extracellular Matrix Proteins in the Anterior Pituitary Gland

Distribution of laminin in the murine pituitary

Immunolocalization of laminin, heparan‐sulfate proteoglycan, entactin, and type IV collagen in the rat anterior pituitary. I. An in vivo study

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