Cellular and molecular responses-mediated by DjMEK1/2 are necessary for planarian regeneration

Wang, Chao; Peng, Rui; Yuan, Xieyong; Liu, Shengpeng; Xu, Shutao; Li, Yan; Zhang, Zhenhua; Zeng, Min; Hu, Lanlin; Zou, Fangdong

doi:10.1016/j.ijbiomac.2020.08.209

Cited by 5 publications

(1 citation statement)

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“…The second wave commences at 48 h and tends to be restricted to the wound site [ 47 ]. Therefore, we performed whole-mount immunofluorescence for phosphorylated histone H3 (H3p) and bromodeoxyuridine (BrdU) labeling experiments, and quantified the mitotic density at 6 and 48 h to determine whether the proliferative ability of neoblasts was impaired after DjMeis1 RNAi [ 48 , 49 ]. However, we observed that DjMeis1 RNAi animals showed normal proliferative ability compared to the control groups at both 6 and 48 h after amputation ( Figure 3 B–E).…”

Section: Resultsmentioning

confidence: 99%

Meis1 Controls the Differentiation of Eye Progenitor Cells and the Formation of Posterior Poles during Planarian Regeneration

Wang

Sun

Liu

et al. 2023

IJMS

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As a member of TALE family, Meis1 has been proven to regulate cell proliferation and differentiation during cell fate commitment; however, the mechanism is still not fully understood. The planarian, which has an abundance of stem cells (neoblasts) responsible for regenerating any organ after injury, is an ideal model for studying the mechanisms of tissue identity determination. Here, we characterized a planarian homolog of Meis1 from the planarian Dugesia japonica. Importantly, we found that knockdown of DjMeis1 inhibits the differentiation of neoblasts into eye progenitor cells and results in an eyeless phenotype with normal central nervous system. Furthermore, we observed that DjMeis1 is required for the activation of Wnt signaling pathway by promoting the Djwnt1 expression during posterior regeneration. The silencing of DjMeis1 suppresses the expression of Djwnt1 and results in the inability to reconstruct posterior poles. In general, our findings indicated that DjMeis1 acts as a trigger for the activation of eye and tail regeneration by regulating the differentiation of eye progenitor cells and the formation of posterior poles, respectively.

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Section: Resultsmentioning

confidence: 99%