Cell Wall Polysaccharides from Chalkumra (Benincasa hispida) Fruit. Part I. Isolation and Characterization of Pectins

Abstract

Pectic polysaccharides were obtained from chalkumra (Benincasa hispida) fruit by sequential extraction with ammonium oxalate (fraction BOX), dilute acid (fraction BHCl), and cold dilute alkali (fraction BOH). The highest yield of polysaccharides was obtained with oxalate and HCl. BOX was enriched in partly methyl-esterified galacturonic acid, whereas BHCl and BOH contained mostly galactose. All of the extracts showed similar elution patterns in size exclusion chromatography although the intrinsic viscosities (eta) were different (132 +/- 6, 100 +/- 5, and 285 + 10 mL/g for BOX, BHCl, and BOH, respectively). From fractionation by anion exchange chromatography, homogalacturonan (as seen from sugar analysis and Fourier transform infrared spectrum) accounted for more than half of BOX and 11% of BHCl. Methylation analyses and hydrolysis of BHCl with endo-beta-(1-->4)-d-galactanase showed the presence of beta-(1-->4)-d-galactan. The neutral galactan represented more than 76% of BHCl and approximately 40% of BOH. The other polysaccharides were complex galactans in BOH and an acidic arabinan (<1%) in BOX and BHCl.

Identification of polysaccharides from pericarp tissues of litchi (Litchi chinensis Sonn.) fruit in relation to their antioxidant activities

“…Polysaccharides are generally present in the cellular walls of fruits and vegetables, [1][2][3] and these can give a thickening, stabilizing or gelling effect to maintain fruit texture. 3 In addition, most polysaccharides that are used in the industry are from plant origins.…”

“…3 In addition, most polysaccharides that are used in the industry are from plant origins. 1,4,5 Litchi (Litchi chinensis Sonn.)…”

Cough and Arabinogalactan Polysaccharide from the Bark of Terminalia Arjuna

“…Neutral sugars were quantified after acid hydrolysis (2 M trifluoroacetic acid, 2 h at 100 C) of TA-P as its alditol acetate derivatives (Blakeney et al 1983) by gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS) at 70 eV, with inositol as an internal standard, using a Shimadzu QP 5050A GC-MS apparatus (Shimadzu; Tokyo, Japan). The GC-MS conditions were as previously described (Mazumder et al 2004). Acid-liberated monosaccharides were also analyzed by thinlayered chromatography on a Kieselgel 60 F plate (Merck; Darmstadt, Germany) using nBuOH/(CH 3 ) 2 CO/pyridine/H 2 O (2/2/1/1 v/v) as an eluent.…”

Revelation of β-sitosterol from Benincasa hispida Seeds, Carissa congesta Roots and Polyalthia longifolia Leaves by High Performance Liquid Chromatography

“…Sequential extraction and high-speed counter current chromatography have yielded components of peptic polysaccharides and astilbin, catechin and naringenin respectively. [5][6][7][8][9][10] In ayurveda, Polyalthia longifolia is well known its preparations such as Kula, Sitaphala kula, Kaphapitashama, Anulomak, Krimighna, Pramehahara and many other allied remedies. [11][12][13] Various constituents have been identified from the leaves like as azafluorene and Aporphine N-oxide alkaloids.…”