Cell-wall lectins during winter wheat cold hardening

Garaeva, L. D.; Pozdeeva, S. A.; Timofeeva, O. A.; Khokhlova, L. P.

doi:10.1134/s1021443706060033

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…During the cold hardening of winter wheat ( Triticum aestivum L., cv. Mironovskaya 808), a transient appearance of 37-and 69-kD lectins, which were identified as AGPs, might indicate their involvement in triggering plant cell defense responses to LT stresses (Garaeva et al, 2006 ). The overexpression of flower specific PRP OsPRP3 conferred the cold tolerance in rice (Gothandam et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress

Yan

Takáč

et al. 2015

Front. Plant Sci.

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Information on the spatial distribution of arabinogalactan proteins (AGPs) in plant organs and tissues during plant reactions to low temperature (LT) is limited. In this study, the extracellular distribution of AGPs in banana leaves and roots, and their changes under LT stress were investigated in two genotypes differing in chilling tolerance, by immuno-techniques using 17 monoclonal antibodies against different AGP epitopes. Changes in total classical AGPs in banana leaves were also tested. The results showed that AGP epitopes recognized by JIM4, JIM14, JIM16, and CCRC-M32 antibodies were primarily distributed in leaf veins, while those recognized by JIM8, JIM13, JIM15, and PN16.4B4 antibodies exhibited predominant sclerenchymal localization. Epitopes recognized by LM2, LM14, and MAC207 antibodies were distributed in both epidermal and mesophyll cells. Both genotypes accumulated classical AGPs in leaves under LT treatment, and the chilling tolerant genotype contained higher classical AGPs at each temperature treatment. The abundance of JIM4 and JIM16 epitopes in the chilling-sensitive genotype decreased slightly after LT treatment, and this trend was opposite for the tolerant one. LT induced accumulation of LM2- and LM14-immunoreactive AGPs in the tolerant genotype compared to the sensitive one, especially in phloem and mesophyll cells. These epitopes thus might play important roles in banana LT tolerance. Different AGP components also showed differential distribution patterns in banana roots. In general, banana roots started to accumulate AGPs under LT treatment earlier than leaves. The levels of AGPs recognized by MAC207 and JIM13 antibodies in the control roots of the tolerant genotype were higher than in the chilling sensitive one. Furthermore, the chilling tolerant genotype showed high immuno-reactivity against JIM13 antibody. These results indicate that several AGPs are likely involved in banana tolerance to chilling injury.

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Section: Discussionmentioning

confidence: 99%

Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress

Yan

Takáč

et al. 2015

Front. Plant Sci.

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“…Another interesting protein that was expressed only under stress conditions was mannose lectin (CaE-202). There are reports suggesting that cell wall lectins may be involved in compensatory mechanisms, which stabi- Dehydration-responsive Chickpea ECM Proteome lize the cytoskeleton structure under stress conditions (44). Furthermore aldolases (CaE-35 and -59) are known to act as adhesion molecules between ECM and the cytoskeleton and may be involved in cross-talk between the extracellular space and the cell interior (45).…”

Section: Screening Of Chickpea Varieties For Dehydrationmentioning

confidence: 99%

Comparative Proteomics Analysis of Differentially Expressed Proteins in Chickpea Extracellular Matrix during Dehydration Stress

Bhushan

Pandey

Choudhary

et al. 2007

Molecular & Cellular Proteomics

192

193

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Water deficit or dehydration is the most crucial environmental factor that limits crop productivity and influences geographical distribution of many crop plants. It is suggested that dehydration-responsive changes in expression of proteins may lead to cellular adaptation against water deficit conditions. Most of the earlier understanding of dehydration-responsive cellular adaptation has evolved from transcriptome analyses. By contrast, comparative analysis of dehydration-responsive proteins, particularly proteins in the subcellular fraction, is limiting. In plants, cell wall or extracellular matrix (ECM) serves as the repository for most of the components of the cell signaling process and acts as a frontline defense. Thus, we have initiated a proteomics approach to identify dehydrationresponsive ECM proteins in a food legume, chickpea. Several commercial chickpea varieties were screened for the status of dehydration tolerance using different physiological and biochemical indexes. Dehydration-responsive temporal changes of ECM proteins in JG-62, a relatively tolerant variety, revealed 186 proteins with variance at a 95% significance level statistically. The comparative proteomics analysis led to the identification of 134 differentially expressed proteins that include predicted and novel dehydration-responsive proteins. This study, for the first time, demonstrates that over a hundred ECM proteins, presumably involved in a variety of cellular functions, viz. cell wall modification, signal transduction, metabolism, and cell defense and rescue, impinge on the molecular mechanism of dehydration tolerance in plants.

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“…Agglutinins have the ability to bind N-acetyl-glucosamine and appear to play a role in defense against pathogens that contain chitin (Chrispeels and Raikhel 1991). An increase in the agglutinin content induced by ABA and induced under several environmental conditions, including cold, has been reported in wheat, suggesting the involvement of agglutinin in tolerance to abiotic stresses (Cammue et al 1989;Garaeva et al 2006). The expression of cTHI in MF was about five times that in PR in the EST analysis; this was confirmed by quantitative RT-PCR.…”

Section: Comparison Of Transcripts In Mf and Pr During Cold Acclimationmentioning

confidence: 99%

Next‐generation sequencing for comparative transcriptomics of perennial ryegrass (Lolium perenne L.) and meadow fescue (Festuca pratensis Huds.) during cold acclimation

Tamura

Yonemaru

2010

Grassland Science

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Perennial ryegrass (PR; Lolium perenne L.) and meadow fescue (MF; Festuca pratensis Huds.) are important grass species for forage production around the world. These species are genetically related but show different responses to biotic and abiotic stresses such as freezing: PR is severely damaged by cold stress, but MF is not. Recently, next‐generation sequencing technology (e.g. 454 sequencing) has been used to profile the genome‐wide transcriptomics of various plant species. To clarify the genetic differences behind the different cold responses of MF and PR, we developed an effective method for comparing transcriptome data during cold acclimation. The expressed sequence tags (ESTs) from cold‐acclimated MF and PR were obtained by means of minimum‐scale 454 sequencing runs. After trimming based on quality scores, these redundant ESTs were assembled into a total of 1527 contigs and singletons for MF and 1060 for PR. Subsequently, we clustered the contigs and singletons into 1082 (MF) and 661 (PR) clusters based on sequence similarity, and we used the EST numbers for the corresponding clusters to compare levels of gene expression. We observed four clusters (wheat germ agglutinin isolectin, γ‐thionin, jasmonate‐induced protein homolog and germin) with markedly different numbers of ESTs between MF and PR. The species‐specific expression of genes corresponding to these four clusters was confirmed by means of reverse‐transcriptase polymerase chain reaction analysis.

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Cell-wall lectins during winter wheat cold hardening

Cited by 9 publications

References 13 publications

Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress

Variable content and distribution of arabinogalactan proteins in banana (Musa spp.) under low temperature stress

Comparative Proteomics Analysis of Differentially Expressed Proteins in Chickpea Extracellular Matrix during Dehydration Stress

Next‐generation sequencing for comparative transcriptomics of perennial ryegrass (Lolium perenne L.) and meadow fescue (Festuca pratensis Huds.) during cold acclimation

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