Comparative Proteomics Analysis of Differentially Expressed Proteins in Chickpea Extracellular Matrix during Dehydration Stress

Abstract

Water deficit or dehydration is the most crucial environmental factor that limits crop productivity and influences geographical distribution of many crop plants. It is suggested that dehydration-responsive changes in expression of proteins may lead to cellular adaptation against water deficit conditions. Most of the earlier understanding of dehydration-responsive cellular adaptation has evolved from transcriptome analyses. By contrast, comparative analysis of dehydration-responsive proteins, particularly proteins in the subcellular fraction, is limiting. In plants, cell wall or extracellular matrix (ECM) serves as the repository for most of the components of the cell signaling process and acts as a frontline defense. Thus, we have initiated a proteomics approach to identify dehydrationresponsive ECM proteins in a food legume, chickpea. Several commercial chickpea varieties were screened for the status of dehydration tolerance using different physiological and biochemical indexes. Dehydration-responsive temporal changes of ECM proteins in JG-62, a relatively tolerant variety, revealed 186 proteins with variance at a 95% significance level statistically. The comparative proteomics analysis led to the identification of 134 differentially expressed proteins that include predicted and novel dehydration-responsive proteins. This study, for the first time, demonstrates that over a hundred ECM proteins, presumably involved in a variety of cellular functions, viz. cell wall modification, signal transduction, metabolism, and cell defense and rescue, impinge on the molecular mechanism of dehydration tolerance in plants.

Superoxide dismutase isozyme activity and antioxidant responses of hydroponically cultured Lepidium sativum L. to NaCl stress

“…Previous works showed that salt tolerance is closely related to the efficiency of antioxidant enzymes (Khan et al 2009;Shaheen et al 2012). SOD and POD are among the major antioxidant enzymes involved in scavenging ROS (Askari et al 2006;Bhushan et al 2007). Our data support this hypothesis since clearly displaying significantly increased SOD and POD isoforms activities in shoots and roots of L. sativum plants cultivated under increasing salinity.…”

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

“…The dehydration responsive nuclear proteome of chickpea revealed a coordinated response, which involves both the regulatory as well as functional proteins. Comparative analysis of differentially expressed proteins in chickpea extracellular matrix during dehydration stress demonstrates that over a hundred ECM proteins, impinge on the molecular mechanism of dehydration tolerance in plants (Bhushan et al, 2007). Plomion et al (2006) mapped the proteome of poplar, discovered certain drought stress responsive proteins and compared with transcriptomic data.…”

Comparative Analyses of Extracellular Matrix Proteome: An Under-Explored Area in Plant Research

“…cell wall modification, signal transduction, cellular transport, metabolism, cell defence and rescue, all of which impinge on the complexity of ECM proteins in crop plants (Bhushan et al, 2006;Telewski, 2006). In recent years, reports have also been published focusing on changes in the ECM proteome in varied cellular events (Jones et al, 2004;Irshad et al, 2008, Bhushan et al, 2007, Cheng et al, 2009, Bhushan et al, 2011. The proteins that have been identified reveal the presence of complex regulatory networks that function in this organelle.…”

“…We analysed the cell wall proteomes of Arabidopsis and maize in response to osmotic stress (Kachroo et al, 2001;Amaya et al, 1999), and the dehydration responsive ECM proteomes of chickpea and rice (Bhushan et al, 2007;Pandey et al, 2008;Choudhary et al, 2009;Bhushan et al, 2011). Interestingly, a great deal of divergence in the protein classes amongst these organisms was observed (Fig.…”