Cell wall degrading enzymes and their impact on Fusarium proliferatum pathogenicity

Sharafaddin, Anwar H.; Hamad, Younis K.; El_Komy, Mahmoud H.; Ibrahim, Yasser E.; Widyawan, Arya; Molan, Younes Y.; Saleh, Amgad A.

doi:10.1007/s10658-019-01818-8

Cited by 14 publications

(8 citation statements)

References 45 publications

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“…As the F. proliferatum was the dominant Fusarium sp. recovered from Saudi date palms, four potent pathogenic strains of F. proliferatum and one strain of F. oxysporum were further selected to infect six-month-old Sheeshee, Khalas and Ruziz seedlings under greenhouse conditions [7,21]. PDA disks (5 mm in diameter) were cut from the edges of 5-day old cultures of Fusarium and transferred to 500-mL flasks containing 150 mL of PDB.…”

Section: Plos Onementioning

confidence: 99%

Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm

et al. 2021

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Several species of Fusarium cause serious diseases in date palm worldwide. In the present work, 14 SSR markers were used to assess the genetic variation of Fusarium strains isolated from diseased trees in Saudi Arabia. We also studied the effect of different temperatures on mycelial growth of these strains. The pathogenicity of four strains of F. proliferatum was also evaluated on local date palm cultivars. Eleven SSR markers amplified a total of 57 scorable alleles from Fusarium strains. Phylogenetic analysis showed that F. proliferatum strains grouped in one clade with 95% bootstrap value. Within F. proliferatum clade, 14 SSR genotypes were identified, 9 of them were singleton. Four out of the five multi-individual SSR genotypes contained strains isolated from more than one location. Most F. solani strains grouped in one clade with 95% bootstrap value. Overall, the SSR markers previously developed for F. verticillioides and F. oxysporum were very useful in assessing the genetic diversity and confirming the identity of Saudi Fusarium strains. The results from the temperature study showed significant differences in mycelial growth of Fusarium strains at different temperatures tested. The highest average radial growth for Fusarium strains was observed at 25°C, irrespective of species. The four F. proliferatum strains showed significant differences in their pathogenicity on date palm cultivars. It is anticipated that the assessment of genetic diversity, effect of temperature on hyphal growth and pathogenicity of potent pathogenic Fusarium strains recovered from date palm-growing locations in Saudi Arabia can help in effectively controlling these pathogens.

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Section: Plos Onementioning

confidence: 99%

Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm

et al. 2021

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“…The results of the mating study are an indication of biological species which showed that morphologically identified as F. proliferatum were certainly F. proliferatum. A high number of successful crosses was also reported by Armengol et al (2005) where 34 from 36 isolates of F. proliferatum isolated from ornamental palms were categorized as MP-D. Several studies also showed similar results where a high number of MP-D crosses has been reported on several host plants such as asparagus (Bargen et al, 2009), date palm (Sharafaddin et al, 2019), onion and garlic (Stankovic et al, 2007;Salvalaggio & Ridao, 2013), rice (Matic ét al., 2013) and corn kernels (Li et al, 2019).…”

Section: Discussionmentioning

confidence: 56%

MOLECULAR CHARACTERIZATION AND MATING STUDY OF Fusarium proliferatum FROM VARIOUS HOSTS IN MALAYSIA

Azuddin

Mohd

Nor

et al. 2021

MABJ

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Fusarium proliferatum is a well-known plant pathogenic fungus-infected many crops. The present study was carried out to molecularly identified and characterized morphologically identified F. proliferatum from various hosts and substrates. The species identity of the isolates was verified as F. proliferatum based on TEF-1α sequences and phylogenetic analysis indicated high intraspecific variations. RFLP-IGS analysis also indicated high intraspecific variations of which the isolates were clustered into three RFLP Groups (I, II, and III) comprising 67 IGS haplotypes. Seventy isolates were crossed-fertile and proven to be members of mating population D (MP-D) of Gibberella fujikuroi while four isolates were infertile. A high level of intraspecific variations is vital for F. proliferatum adaptation and survival in the host and environment. Correct species identification of F. proliferatum is important as the fungus is a well-known plant pathogen and mycotoxin producer. Correct species identity is also essential to strategize suitable disease control methods as well as to predict their host range and mycotoxin production.

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“…Most of CWDEs are adaptively secreted by pathogenic fungi in the presence of appropriate substrates. The addition of exogenous inducers could assess the type and activity of CWDEs and analyze the correlation between the enzyme-producing and colonising abilities of pathogenic fungi ( Sharafaddin et al, 2019 ). Plant cell walls are mainly composed of lignin, cellulose, pectin, and hemicellulose ( Dabravolski & Isayenkov, 2023 ).…”

Section: Resultsmentioning

confidence: 99%

Physiological response of chestnuts (Castanea mollissima Blume) infected by pathogenic fungi and their correlation with fruit decay

wen,

Zhu,

Geng

et al. 2024

Food Chemistry: X

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Cell wall degrading enzymes and their impact on Fusarium proliferatum pathogenicity

Cited by 14 publications

References 45 publications

Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm

Molecular and physiological characterization of Fusarium strains associated with different diseases in date palm

MOLECULAR CHARACTERIZATION AND MATING STUDY OF Fusarium proliferatum FROM VARIOUS HOSTS IN MALAYSIA

Physiological response of chestnuts (Castanea mollissima Blume) infected by pathogenic fungi and their correlation with fruit decay

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