Cell Wall Composition and Morphogenic Response in Callus Derived from Protoplasts of two Fibre flax (Linum usitatissimum L.) Genotypes

David, Hélène N.; David, Alain; Bade, Pascale; Millet, Jacques; Morvan, O.; Morvan, Claudine

doi:10.1016/s0176-1617(11)81648-2

Cited by 7 publications

(5 citation statements)

References 29 publications

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“…The presence of high levels of galactose and arabinose indicated that pectins were the main polysaccharides present in these extracts. These findings were consistent with those reported in calli of Linum usitatissimum L. (David et al 1994). In our study, sugar levels were significantly higher in E calli.…”

Section: Resultssupporting

confidence: 93%

Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L.

Endress

Barriuso

Rupérez

et al. 2009

Plant Cell Tiss Organ Cult

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Analysis of cell wall polysaccharide composition of embryogenic and non-embryogenic calli obtained from hypocotyl and petiole explants from Medicago arborea L. revealed significant differences. For calli induced from both hypocotyls and petioles, levels of total sugars, pectins, and hemicelluloses were higher in embryogenic than in non-embryogenic calli. Whereas in the residual cellulose fraction, the highest levels of sugar were detected in non-embryogenic calli. When comparing the two donor sources of callus explants, the highest total sugar levels were detected in embryogenic calli induced from petioles, mainly in the pectin fraction and to a lesser extent in the hemicellulose fraction. Moreover, analysis of uronic acids revealed higher levels in embryogenic calli, primarily in the pectin fraction. Analysis of those sugars associated with cell walls of calli suggested that these polysaccharides consisted of pectic polysaccharides and glucans, and that their levels were higher in embryogenic than non-embryogenic calli.

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Section: Resultssupporting

confidence: 93%

Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L.

Endress

Barriuso

Rupérez

et al. 2009

Plant Cell Tiss Organ Cult

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“…After filtration, BY‐2 cells were washed once with ethanol, once with chloroform‐methanol 1:1 (v/v) and twice with acetone according to David et al (1994). Crude cell walls were dried for 2 h at 45°C and then for 1 h at 80°C.…”

Section: Methodsmentioning

confidence: 99%

Cloning of a pine germin‐like protein (GLP) gene promoter and analysis of its activity in transgenic tobacco Bright Yellow 2 cells

Mathieu¹,

Neutelings²,

Hawkins

et al. 2003

Physiologia Plantarum

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Germins and germin-like proteins (GLPs) constitute a large and highly diverse family of ubiquitous plant cell wall proteins. These proteins seem to be involved in many developmental stages and stress-related processes, but their exact participation in these processes generally remains obscure. In Pinus caribaea Morelet, the PcGER1 gene is expressed uniquely in embryo tissues, and encodes a GLP ionically bound to the walls of pine embryo cells maintained in 2,4-D-containing medium. We have cloned a genomic fragment including the 1520 bp 5'-upstream promoter region of PcGER1. This sequence contains, in its 1200 bp distal part, several cis elements (e.g. SEF4, 60 kDa protein, ABA RE and Dof recognition sites) present in genes responding to hormones and/or expressed in embryo or seed tissues, or during germination. The PcGER1 promoter sequence was cloned upstream of the GUS (beta-glucuronidase) reporter gene and transferred to tobacco Bright Yellow 2 (BY-2) cells via Agrobacterium tumefaciens-mediated transformation. Promoter activity and growth performances of transgenic asynchronous cell suspensions were analysed in the presence or absence of 2,4-D and/or BA. Optimal growth, maximum cell-wall yield and PcGER1 promoter activity were observed in the presence of 2,4-D and BA at day 4, the end of the exponential growth phase where 70-75% cells have a 2C DNA content. Analysis of promoter activity during the cell cycle in an aphidicoline-synchronized culture suggested that the expression is maximum in G1 cells. We also showed that under optimal growth conditions, 5' promoter deletions decreased the activity of the reporter gene. We discuss the function of this gene with regards to cell growth. Accession number: The PcGER1 promoter sequence was submitted to the genbank database under the accession number AY077704.

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“…Intact plant regeneration would appear, by the lack of publications, to be problematic in flax, nevertheless flax protoplasts have been widely used in a number of biochemical and physiological studies, see Table 2. Evidence of the difficulty of obtaining intact plants from protoplasts was made by a study of the cell wall composition and morphogenic response in callus derived from protoplasts of fibre flax, reported by David et al (1994). However, ELS formation from protoplasts of flax was described by Roger et al (1998).…”

Section: Protoplastsmentioning

confidence: 99%

Plant cell and biotechnology studies in Linum usitatissimum – a review

Millam¹,

Obert

Preťová

2005

Plant Cell Tiss Organ Cult

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The species Linum usitatissimum (flax/linseed) has been the focus of a great deal of both basic and applied research effort in plant cell and biotechnology studies in recent years. In this review we consider applications of the techniques of plant biotechnology in this species under several distinct headings. Plant cell and tissue regeneration strategies and applications are discussed, and the applications of the techniques of somatic embryogenesis, protoplast isolation, culture and fusion and cell suspension cultures in this species are described. A major area of study is the use of anther and microspore culture where clear advantages to breeding programmes could be applied. In addition, embryo and ovary culture studies have resulted in significant findings. The more recent technologies of gene transfer and expression by genetic transformation are reviewed, and a section on strategies for improvements in technological quality is also included. Finally we propose conclusions and future prospects for this ancient, but still highly relevant crop.

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Cell Wall Composition and Morphogenic Response in Callus Derived from Protoplasts of two Fibre flax (Linum usitatissimum L.) Genotypes

Cited by 7 publications

References 29 publications

Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L.

Differences in cell wall polysaccharide composition between embryogenic and non-embryogenic calli of Medicago arborea L.

Cloning of a pine germin‐like protein (GLP) gene promoter and analysis of its activity in transgenic tobacco Bright Yellow 2 cells

Plant cell and biotechnology studies in Linum usitatissimum – a review

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