Cell wall component and mycotoxin moieties involved in the binding of fumonisin B₁and B₂by lactic acid bacteria

Abstract

Aims: The ability of lactic acid bacteria (LAB) to bind fumonisins B1 and B2 (FB1, FB2) in fermented foods and feeds and in the gastrointestinal tract could contribute to decrease their bioavailability and toxic effects on farm animals and humans. The aim of this work was to identify the bacterial cell wall component(s) and the functional group(s) of FB involved in the LAB–FB interaction. Methods and Results: The effect of physicochemical, enzymatic and genetic treatments of bacteria and the removal/inactivation of the functional groups of FB on toxin binding were evaluated. Treatments affecting the bacterial wall polysaccharides, lipids and proteins increased binding, while those degrading peptidoglycan (PG) partially decreased it. In addition, purified PG from Gram‐positive bacteria bound FB in a manner analogue to that of intact LAB. For FB, tricarballylic acid (TCA) chains play a significant role in binding as hydrolysed FB had less affinity for LAB. Conclusions: Peptidoglycan and TCA are important components of LAB and FB, respectively, involved in the binding interaction. Significance and Impact of the Study: Lactic acid bacteria binding efficiency seems related to the peptide moiety structure of the PG. This information can be used to select probiotics with increased FB binding efficiency.

Potential of lactic acid bacteria for the reduction of fumonisin exposure in African fermented maize based foods

“…It was demonstrated that binding is the main mechanism involved in fumonisin removal, with adhesion to LAB cell wall components occurring rather than covalent binding or metabolism (Dalié et al, 2010;Niderkorn et al, 2009;Zhao et al, 2016). Our preliminary results were in agreement with binding mechanism since fumonisin removal was observed in presence of both live and dead LAB (unpublished data).…”

“…and FB2, varies with bacterial species (Niderkorn et al, 2009;Zhao et al, 2016). with all the strains exhibiting higher binding for FB2 than FB1.Binding ratio increased with a decline in pH from 6 to 2 and an increase in incubation temperature from 30°C to 37°C.…”

“…LAB grows optimally at 37°C where the cells prepare for growth either by "make before break" or "inside to outside" principle as explained by Delcour et al (1999). In both principles, more peptidoglycans are added to the cell wall (Delcour et al, 1999) providing more favourable binding sites for FB1 and FB2 (Delcour et al, 1999;Niderkorn et al, 2009;Zhao et al, 2016), hence enhanced binding.…”

“…LAB strains are Gram positive and so their cell wall mainly consists of peptidoglycans, teichoic acids, proteins and polysaccharides (Chapot-Chartier and Kulakauskas, 2014;Delcour et al, 1999).It was reported that peptidoglycans of LAB cell wall are the main binding sites for fumonisins, their structural integrity is essential and their amino acid sequence plays an important role in the efficiency of the binding mechanism. TCA chains in fumonisins also are important components in the binding process (Dalié et al, 2010;Niderkorn et al, 2009;Zhao et al, 2016).…”

Myco-toxins Monitoring Device and their Management Strategies through Detoxifying Agents in Feed

“…(Galvano et al,1998), (Nageswara and Chopra, 2001) (Avantaggiato et al,2007) (Bursian, 2004), (Diaz et al,2004), (Chowdhury and Smith, 2005), (Kogan and Kocher, 2007), (Dvorska, 2007), (Smith et al,2008), (Moschini et al,2008), fusaric acid (Meissonnier et al,2009) ( Niderkorn et al,2006), (Niderkorn et al,2007), (Niderkorn et al,2008), (Niderkorn et al,2009) 20g/l OTA REALDYME (Tangni 2003, (Tangni et al,2005, (Aoudia et al,2008), (Aoudia et al,2009) 13. Antitox Vana (Polyvinylpolypyrrodilone, C 6 H 9 NO) 20g/l DON Qualitech Products Inc. (Friend, 1984) 2.…”