Cell-wall-bound metal ions are not taken up inNeurospora crassa

Abstract

To establish the relevance of the cell wall in metal ion transport, cobalt uptake was examined in Neurospora crassa. Cobalt taken up was largely surface bound (>90%), resulting in a release of calcium and magnesium. Surface-bound cobalt could not enter intracellular locations upon further incubation of mycelia in a metal-free medium. Saturation of the surface with one metal augured subsequent dose-dependent entry of a different metal into intracellular locations. In comparison with the cobalt-resistant mutant, the cobalt-sensitive strain of N. crassa bound less cobalt on the surface but with significant intracellular accumulation. Our results demonstrate the importance of the cell wall in metal transport, toxicity, and resistance in fungi.

Specific localization of inorganic polyphosphate (poly P) in fungal cell walls by selective extraction and immunohistochemistry

“…Nevertheless, poly P appeared to be an important component in the cell wall of M. mucedo, since even mycelium grown on phosphate free medium contained poly P as indicated by the speciWc staining. Cell wall localized poly P could also be involved in the sequestration of cations (Kornberg et al, 1999) or the protection from the toxic eVects of heavy metals (Latha et al, 2005) and exerts antimicrobial activity against many bacteria or fungi (Jen and Shelef, 1986;Maier et al, 1999). The detection of poly P in cell walls of an arbuscular mycorrhizal fungus (G. intraradices) as well as two ectomycorrhizal fungi (P. panuoides and P. arhizus) might also indicate a role of cell wall localized poly P in the phosphate transfer in mycorrhizal interactions.…”

Adsorption profile of lead on Aspergillus versicolor: A mechanistic probing

“…Volesky [14] has recently reviewed the state of art in the field of biosorption of heavy metals. However, only a few reports are available on fungal systems [15,16]. Fungal biomass has certain advantages over bacterial biomass in respect of processing and handling of the biomass.…”

Biosorption of hexavalent chromium by Termitomyces clypeatus biomass: Kinetics and transmission electron microscopic study

“…The state of art in the field of biosorption of heavy metals has recently been reviewed by Volesky [23]. However, only a few reports are available on fungal systems [24,25]. Fungal biomass has certain advantage over bacterial biomass in this natural 'ecofriendly green technological process' in respect of processing and handling of the biomass.…”