Cell wall biosynthesis: glycan containing oligomers in developing cotton fibers, cotton fabric, wood and paper

Murray, Allen K.; Nichols, Robert L.; Sassenrath, Gretchen F.

doi:10.1016/s0031-9422(01)00143-1

Cited by 7 publications

(5 citation statements)

References 20 publications

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“…By 30 DPA, the amounts of Ara and Rha in the hot water-soluble fraction cell wall fraction had increased significantly compared with 17 DPA (P # 0.05), and Gal and GalA showed the same trend (Supplemental Table S3). The CFML had physically disappeared several days earlier, but remnant sugars or oligomers could have persisted within the closed boll compartment and been hot water extractable, a possibility that is supported by the increase in transition-stage fiber of soluble carbohydrate oligomers detected by HPLC (Murray et al, 2001). …”

Section: Transition-stage Cfml Breakdown Was Correlated With Changes mentioning

confidence: 92%

“…Because the CFML breaks down before secondary wall deposition begins, a strong rationale now exists for changes in cell wall polymers and cell wall hydrolytic activity at the transition stage (Meinert and Delmer, 1977;Shimizu et al, 1997;Tokumoto et al, 2002Tokumoto et al, , 2003Guo et al, 2007;this study). CFML hydrolysis may also explain the increasing extractability of glycan oligomers near the onset of secondary wall deposition in cotton fiber (Murray et al, 2001).…”

Section: Relationship Between the Cotton Fiber Tissue And Cuticlementioning

confidence: 99%

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A Specialized Outer Layer of the Primary Cell Wall Joins Elongating Cotton Fibers into Tissue-Like Bundles

et al. 2009

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Cotton (Gossypium hirsutum) provides the world's dominant renewable textile fiber, and cotton fiber is valued as a research model because of its extensive elongation and secondary wall thickening. Previously, it was assumed that fibers elongated as individual cells. In contrast, observation by cryo-field emission-scanning electron microscopy of cotton fibers developing in situ within the boll demonstrated that fibers elongate within tissue-like bundles. These bundles were entrained by twisting fiber tips and consolidated by adhesion of a cotton fiber middle lamella (CFML). The fiber bundles consolidated via the CFML ultimately formed a packet of fiber around each seed, which helps explain how thousands of cotton fibers achieve their great length within a confined space. The cell wall nature of the CFML was characterized using transmission electron microscopy, including polymer epitope labeling. Toward the end of elongation, up-regulation occurred in gene expression and enzyme activities related to cell wall hydrolysis, and targeted breakdown of the CFML restored fiber individuality. At the same time, losses occurred in certain cell wall polymer epitopes (as revealed by comprehensive microarray polymer profiling) and sugars within noncellulosic matrix components (as revealed by gas chromatography-mass spectrometry analysis of derivatized neutral and acidic glycosyl residues). Broadly, these data show that adhesion modulated by an outer layer of the primary wall can coordinate the extensive growth of a large group of cells and illustrate dynamic changes in primary wall structure and composition occurring during the differentiation of one cell type that spends only part of its life as a tissue.

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Section: Transition-stage Cfml Breakdown Was Correlated With Changes mentioning

confidence: 92%

Section: Relationship Between the Cotton Fiber Tissue And Cuticlementioning

confidence: 99%

A Specialized Outer Layer of the Primary Cell Wall Joins Elongating Cotton Fibers into Tissue-Like Bundles

et al. 2009

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“…Sugars act as signaling molecules that influence the function of promoters and transcription factors (Ruan, 2012). Sugar, together with cellulose, is crucial for lint length and strength in cotton at the time of secondary cell wall synthesis (Murray et al, 2001). Cellulose synthesis starts at the end of primary wall formation, and its accumulation is pivotal at the time of secondary wall synthesis.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Sucrose Synthase Gene Indirectly Improves Cotton Fiber Quality Through Sucrose Cleavage

et al. 2020

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The study aims to improve fiber traits of local cotton cultivar through genetic transformation of sucrose synthase ( SuS ) gene in cotton. Sucrose synthase (SuS) is an important factor that is involved in the conversion of sucrose to fructose and UDP-glucose, which are essential for the synthesis of cell wall cellulose. In the current study, we expressed a synthetic SuS gene in cotton plants under the control of a CaMV35S promoter. Amplification of an 813-bp fragment using gene-specific primers confirmed the successful introduction of SuS gene into the genome of cotton variety CEMB-00. High SuS mRNA expression was observed in two transgenic cotton plants, MA0023 and MA0034, when compared to the expression in two other transgenic cotton plants, MA0035 and MA0038. Experiments showed that SuS mRNA expression was positively correlated with SuS activity at the vegetative (54%) and reproductive stages (40%). Furthermore, location of transgene was found to be at chromosome no. 9 in the form of single insertion, while no signal was evident in non-transgenic control cotton plant when evaluated through fluorescent in situ hybridization and karyotyping analysis. Fiber analyses of the transgenic cotton plants showed increases of 11.7% fiber length, 18.65% fiber strength, and up to 5% cellulose contents. An improvement in the micronaire value of 4.21 was also observed in the MA0038 transgenic cotton line. Scanning electron microscopy (SEM) revealed that the fibers of the SuS transgenic cotton plants were highly spiral with a greater number of twists per unit length than the fibers of the non-transgenic control plants. These results determined that SuS gene expression influenced cotton fiber structure and quality, suggesting that SuS gene has great potential for cotton fiber quality improvement.

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“…Fibre development consists of four overlapping stages: initiation, elongation, secondary wall deposition and maturation (Qin and Zhu 2011). Sugar and cellulose are considered to be the key modulators of cotton fibre length and strength during the secondary cell wall deposition phase (Murray et al 2001). Cellulose biosynthesis begins during the end of primary cell wall extension and is most important during the secondary cell wall biosynthesis period, which is followed by a dehydration period that produces mature lint fibres (Qin and Zhu 2011).…”

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confidence: 99%

Over‐expression of GhUGP1 in upland cotton improves fibre quality and reduces fibre sugar content

Yang

et al. 2015

Plant Breeding

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GhUGP1 (cotton uridine diphosphate glucose pyrophosphorylase) is one of the most highly expressed genes and proteins in fast elongation cotton fibres. The objective of this study was to determine whether fibre quality and reduced sugar content could be improved in fibres by over-expressing GhUGP1 in cotton plants under conditions of low autumn temperatures and large diurnal temperature differences. A plant GhUGP1 vector was constructed and introduced into the 3-6-7 cotton line using agrobacterium-mediated transformation. Following selection with kanamycin sulphate and polymerase chain reaction (PCR) and Southern blotting analyses, three independent homozygous transgenic lines were selected for molecular and chemical study. Compared with wild type, the transcriptional activity of GhUGP1 was higher and more long lasting in the transgenic lines. Fibres were longer and stronger, soluble and reduced sugar levels were significantly decreased, and cellulose levels were significantly increased. In addition, plant height also increased by 40.1-45.1%. These results demonstrate that GhUGP1 expression was considerably increased in the transgenic plants, accelerating the conversion of oligosaccharides to polysaccharides in the developing cotton fibre.

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Cell wall biosynthesis: glycan containing oligomers in developing cotton fibers, cotton fabric, wood and paper

Cited by 7 publications

References 20 publications

A Specialized Outer Layer of the Primary Cell Wall Joins Elongating Cotton Fibers into Tissue-Like Bundles

A Specialized Outer Layer of the Primary Cell Wall Joins Elongating Cotton Fibers into Tissue-Like Bundles

Overexpression of a Sucrose Synthase Gene Indirectly Improves Cotton Fiber Quality Through Sucrose Cleavage

Over‐expression of GhUGP1 in upland cotton improves fibre quality and reduces fibre sugar content

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